LOCUS       sequence-264937-       11353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of Ecad in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11353)
  AUTHORS   Toda S, Blauch LR, Tang SKY, Morsut L, Lim WA
  TITLE     Programming self-organizing multicellular structures with synthetic 
            cell-cell signaling.
  JOURNAL   Science. 2018 Jul 13;361(6398):156-162. doi: 
            10.1126/science.aat0271. Epub 2018 May 31.
  PUBMED    29853554
REFERENCE   2  (bases 1 to 11353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aat0271"; journalName: "Science"; date: 
            "2018-07-13- 13"; volume: "361"; issue: "6398"; pages: "156-162"
COMMENT     Sequence Label: pHR_UAS-Ecad
FEATURES             Location/Qualifiers
     source          1..11353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1257..1322
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1452..1472
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1897..2031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1934..1953)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1988..2007
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2199..2832
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2879..3004
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3501..3734
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3919..3963
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4248..4365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     4591..4615
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    7415..8003
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7468..7488)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7886..7897)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8006..8022)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8007..8023)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8113..8346
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8371..8389)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8372..8389)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8679..8698)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8912..8931)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9031..9053
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9091..9109)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9177..9281
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9282..9350,9351..10142)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9500..9519)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10313..10901
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10802..10821
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11055..11072
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11142..11162)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(11147..11353,1..123)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(11327..11353,1..109)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
       61 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      121 aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc
      181 gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc
      241 gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga
      301 taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca
      361 gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat
      421 tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt
      481 catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg
      541 ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc
      601 ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc
      661 gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc
      721 tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa
      781 tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata
      841 gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct
      901 gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg
      961 cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat
     1021 gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca
     1081 ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat
     1141 tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt
     1201 acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa
     1261 atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt
     1321 agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa
     1381 acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac
     1441 attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat
     1501 tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt
     1561 acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa
     1621 cctttataag taggcataac agttataatc ataacatact gttttttctt actccacaca
     1681 ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa
     1741 tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc
     1801 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     1861 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     1921 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1981 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
     2041 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
     2101 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
     2161 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
     2221 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     2281 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     2341 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     2401 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     2461 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     2521 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     2581 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
     2641 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     2701 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     2761 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     2821 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     2881 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     2941 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     3001 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     3061 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     3121 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     3181 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     3241 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     3301 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
     3361 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     3421 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     3481 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     3541 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     3601 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     3661 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     3721 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     3781 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     3841 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     3901 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     3961 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     4021 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     4081 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     4141 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     4201 gtgaacggat ctcgacggta tcgccaaatg gcagtattca tccacaattt taaaagaaaa
     4261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     4321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     4381 gacagcagag atccagtttg gatcgataag cttgatatcg aattcggagc actgtcctcc
     4441 gaacgtcgga gcactgtcct ccgaacgtcg gagcactgtc ctccgaacgt cggagcactg
     4501 tcctccgaac ggagcatgtc ctccgaacgt cggagcactg tcctccgaac gactagttag
     4561 gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     4621 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctctc
     4681 gacattcgtt ggatcgcggc cgcggagcta ccatgggccc ttggagccgc agcctctcgg
     4741 cgctgctgct gctgctgcag gtctcctctt ggctctgcca ggagccggag ccctgccacc
     4801 ctggctttga cgccgagagc tacacgttca cggtgccccg gcgccacctg gagagaggcc
     4861 gcgtcctggg cagagtgaat tttgaagatt gcaccggtcg acaaaggaca gcctattttt
     4921 ccctcgacac ccgattcaaa gtgggcacag atggtgtgat tacagtcaaa aggcctctac
     4981 ggtttcataa cccacagatc catttcttgg tctacgcctg ggactccacc tacagaaagt
     5041 tttccaccaa agtcacgctg aatacagtgg ggcaccacca ccgccccccg ccccatcagg
     5101 cctccgtttc tggaatccaa gcagaattgc tcacatttcc caactcctct cctggcctca
     5161 gaagacagaa gagagactgg gttattcctc ccatcagctg cccagaaaat gaaaaaggcc
     5221 catttcctaa aaacctggtt cagatcaaat ccaacaaaga caaagaaggc aaggttttct
     5281 acagcatcac tggccaagga gctgacacac cccctgttgg tgtctttatt attgaaagag
     5341 aaacaggatg gctgaaggtg acagagcctc tggatagaga acgcattgcc acatacactc
     5401 tcttctctca cgctgtgtca tccaacggga atgcagttga ggatccaatg gagattttga
     5461 tcacggtaac cgatcagaat gacaacaagc ccgaattcac ccaggaggtc tttaaggggt
     5521 ctgtcatgga aggtgctctt ccaggaacct ctgtgatgga ggtcacagcc acagacgcgg
     5581 acgatgatgt gaacacctac aatgccgcca tcgcttacac catcctcagc caagatcctg
     5641 agctccctga caaaaatatg ttcaccatta acaggaacac aggagtcatc agtgtggtca
     5701 ccactgggct ggaccgagag agtttcccta cgtataccct ggtggttcaa gctgctgacc
     5761 ttcaaggtga ggggttaagc acaacagcaa cagctgtgat cacagtcact gacaccaacg
     5821 ataatcctcc gatcttcaat cccaccacgt acaagggtca ggtgcctgag aacgaggcta
     5881 acgtcgtaat caccacactg aaagtgactg atgctgatgc ccccaatacc ccagcgtggg
     5941 aggctgtata caccatattg aatgatgatg gtggacaatt tgtcgtcacc acaaatccag
     6001 tgaacaacga tggcattttg aaaacagcaa agggcttgga ttttgaggcc aagcagcagt
     6061 acattctaca cgtagcagtg acgaatgtgg taccttttga ggtctctctc accacctcca
     6121 cagccaccgt caccgtggat gtgctggatg tgaatgaagc ccccatcttt gtgcctcctg
     6181 aaaagagagt ggaagtgtcc gaggactttg gcgtgggcca ggaaatcaca tcctacactg
     6241 cccaggagcc agacacattt atggaacaga aaataacata tcggatttgg agagacactg
     6301 ccaactggct ggagattaat ccggacactg gtgccatttc cactcgggct gagctggaca
     6361 gggaggattt tgagcacgtg aagaacagca cgtacacagc cctaatcata gctacagaca
     6421 atggttctcc agttgctact ggaacaggga cacttctgct gatcctgtct gatgtgaatg
     6481 acaacgcccc cataccagaa cctcgaacta tattcttctg tgagaggaat ccaaagcctc
     6541 aggtcataaa catcattgat gcagaccttc ctcccaatac atctcccttc acagcagaac
     6601 taacacacgg ggcgagtgcc aactggacca ttcagtacaa cgacccaacc caagaatcta
     6661 tcattttgaa gccaaagatg gccttagagg tgggtgacta caaaatcaat ctcaagctca
     6721 tggataacca gaataaagac caagtgacca ccttagaggt cagcgtgtgt gactgtgaag
     6781 gggccgccgg cgtctgtagg aaggcacagc ctgtcgaagc aggattgcaa attcctgcca
     6841 ttctggggat tcttggagga attcttgctt tgctaattct gattctgctg ctcttgctgt
     6901 ttcttcggag gagagcggtg gtcaaagagc ccttactgcc cccagaggat gacacccggg
     6961 acaacgttta ttactatgat gaagaaggag gcggagaaga ggaccaggac tttgacttga
     7021 gccagctgca caggggcctg gacgctcggc ctgaagtgac tcgtaacgac gttgcaccaa
     7081 ccctcatgag tgtcccccgg tatcttcccc gccctgccaa tcccgatgaa attggaaatt
     7141 ttattgatga aaatctgaaa gcggctgata ctgaccccac agccccgcct tatgattctc
     7201 tgctcgtgtt tgactatgaa ggaagcggtt ccgaagctgc tagtctgagc tccctgaact
     7261 cctcagagtc agacaaagac caggactatg actacttgaa cgaatggggc aatcgcttca
     7321 agaagctggc tgacatgtac ggaggcggcg aggacgacta gcctgcaggc atgcaagctt
     7381 gcaggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     7441 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7501 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7561 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7621 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7681 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7741 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7801 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     7861 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7921 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7981 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct
     8041 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     8101 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     8161 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8221 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8281 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8341 ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat
     8401 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa
     8461 ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag
     8521 agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat
     8581 ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt
     8641 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     8701 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     8761 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     8821 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     8881 taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8941 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     9001 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     9061 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     9121 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     9181 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9241 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     9301 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9361 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9421 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9481 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9541 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9601 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9661 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9721 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9781 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9841 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9901 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9961 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    10021 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    10081 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    10141 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    10201 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    10261 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10321 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    10381 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10441 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10501 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10561 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10621 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10681 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10741 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10801 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10861 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10921 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10981 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    11041 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    11101 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
    11161 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    11221 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    11281 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
    11341 tccgcccagt tcc
//
