LOCUS       sequence-26494-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Cormack BP, Valdivia RH, Falkow S
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP).
  JOURNAL   Gene. 1996 . 173(1 Spec No):33-8.
  PUBMED    8707053
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 1996 
            . 173(1 Spec No):33-8."
COMMENT     Sequence Label: pKEN GFP mut2
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..777
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(94..122)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     717..737
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(841..858)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ataatgngtg gaantcgagc tcggtacccg gggancctct agatttaaga aggagatata
       61 catatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      121 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      181 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      241 cttgtcacta ctttcgcgta tggtcttcaa tgctttgcga gatacccaga tcatatgaaa
      301 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      361 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      421 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      481 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
      541 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      601 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      661 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
      721 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
      781 atgtccagac ctgcaggcat gcaagcttgg ctgttttggc ggatgagaga anantttcag
      841 cctgatacag attaaatcan aacgcanaan cggtctgata aaacanaatt tgcctggcgg
      901 cagtagcgcg gtggnc
//
