LOCUS       sequence-264951-       10876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  repressible expression of CD19 and mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Toda S, Blauch LR, Tang SKY, Morsut L, Lim WA
  TITLE     Programming self-organizing multicellular structures with synthetic 
            cell-cell signaling.
  JOURNAL   Science. 2018 Jul 13;361(6398):156-162. doi: 
            10.1126/science.aat0271. Epub 2018 May 31.
  PUBMED    29853554
REFERENCE   2  (bases 1 to 10876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aat0271"; journalName: "Science"; date: 
            "2018-07-13- 13"; volume: "361"; issue: "6398"; pages: "156-162"
COMMENT     Sequence Label: pHR_TetO_CD19-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..10876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    225..342
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        402..705
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        706..909
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     860..880
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    911..929
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    932..950
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     sig_peptide     1006..1065
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1069..1095
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1933..2079
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             2101..2157
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      2161..2170
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2167..2877
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2312..2330)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2580..2599
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2915..3503
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2968..2988)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3386..3397)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3506..3522)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3507..3523)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3613..3846
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3871..3889)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3872..3889)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4179..4198)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4412..4431)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4531..4553
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4591..4609)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4677..4781
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4782..4850,4851..5642)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5000..5019)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5813..6401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6302..6321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6555..6572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6642..6662)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6647..6976
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6827..6962
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8110..8175
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8305..8325
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8750..8884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8787..8806)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8841..8860
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9052..9685
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9732..9857
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10354..10587
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10772..10816
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
       61 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
      121 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
      181 aacggatctc gacggtatcg ccaaatggca gtattcatcc acaattttaa aagaaaaggg
      241 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
      301 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
      361 agcagagatc cagtttggat cgataagctt gatatcgaat tcgttacata acttacggta
      421 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      481 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      541 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      601 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      661 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      721 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      781 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      841 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      901 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgaatt
      961 ctcacgcgtc tgcaggatat caagcttgcg gtaccgcggg cccggatgga gacagacaca
     1021 ctcctgctat gggtactgct gctctgggtt ccaggttcca ctggtgacta tccatatgat
     1081 gttccagatt atgctggggc ccagccggcc aggcccgagg aacctctagt ggtgaaggtg
     1141 gaagagggag ataacgctgt gctgcagtgc ctcaagggga cctcagatgg ccccactcag
     1201 cagctgacct ggtctcggga gtccccgctt aaacccttct taaaactcag cctggggctg
     1261 ccaggcctgg gaatccacat gaggcccctg gccatctggc ttttcatctt caacgtctct
     1321 caacagatgg ggggcttcta cctgtgccag ccggggcccc cctctgagaa ggcctggcag
     1381 cctggctgga cagtcaatgt ggagggcagc ggggagctgt tccggtggaa tgtttcggac
     1441 ctaggtggcc tgggctgtgg cctgaagaac aggtcctcag agggccccag ctccccttcc
     1501 gggaagctca tgagccccaa gctgtatgtg tgggccaaag accgccctga gatctgggag
     1561 ggagagcctc cgtgtgtccc accgagggac agcctgaacc agagcctcag ccaggacctc
     1621 accatggccc ctggctccac actctggctg tcctgtgggg taccccctga ctctgtgtcc
     1681 aggggccccc tctcctggac ccatgtgcac cccaaggggc ctaagtcatt gctgagccta
     1741 gagctgaagg acgatcgccc ggccagagat atgtgggtaa tggagacggg tctgttgttg
     1801 ccccgggcca cagctcaaga cgctggaaag tattattgtc accgtggcaa cctgaccatg
     1861 tcattccacc tggagatcac tgctcggcca gtactatggc actggctgct gaggactggt
     1921 ggctggaaga atgctgtggg ccaggacacg caggaggtca tcgtggtgcc acactccttg
     1981 ccctttaagg tggtggtgat ctcagccatc ctggccctgg tggtgctcac catcatctcc
     2041 cttatcatcc tcatcatgct ttggcagaag aagccacgta ctagtgccag cggcagcggc
     2101 gccacaaact tctctctgct aaagcaagca ggtgatgttg aagaaaaccc cgggcctgcg
     2161 gccgccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     2221 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     2281 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     2341 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     2401 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     2461 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     2521 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     2581 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     2641 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     2701 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     2761 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     2821 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaacct
     2881 gcaggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     2941 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3001 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3061 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3121 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3181 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3241 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3301 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     3361 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3421 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3481 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct
     3541 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     3601 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     3661 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     3721 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     3781 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     3841 ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat
     3901 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa
     3961 ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag
     4021 agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat
     4081 ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt
     4141 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     4201 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     4261 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     4321 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     4381 taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4441 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4501 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     4561 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     4621 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     4681 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4741 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4801 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4861 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4921 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4981 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5041 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5101 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5161 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5221 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5281 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5341 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5401 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5461 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5521 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5581 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5641 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5701 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5761 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5821 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5881 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5941 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6001 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6061 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6481 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6541 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     6601 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
     6661 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6721 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6781 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6841 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6901 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6961 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
     7021 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     7081 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     7141 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     7201 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     7261 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
     7321 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     7381 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     7441 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     7501 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     7561 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     7621 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     7681 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     7741 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     7801 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     7861 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     7921 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     7981 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
     8041 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     8101 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     8161 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     8221 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     8281 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     8341 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     8401 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     8461 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     8521 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     8581 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     8641 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     8701 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     8761 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8821 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8881 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     8941 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     9001 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
     9061 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     9121 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     9181 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     9241 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     9301 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     9361 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     9421 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     9481 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     9541 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9601 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     9661 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     9721 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     9781 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     9841 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     9901 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     9961 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
    10021 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
    10081 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
    10141 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
    10201 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
    10261 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    10321 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    10381 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    10441 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    10501 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    10561 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    10621 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    10681 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    10741 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    10801 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    10861 attcataatg atagta
//
