LOCUS       sequence-264956-       11540 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of Ecad in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   Toda S, Blauch LR, Tang SKY, Morsut L, Lim WA
  TITLE     Programming self-organizing multicellular structures with synthetic 
            cell-cell signaling.
  JOURNAL   Science. 2018 Jul 13;361(6398):156-162. doi: 
            10.1126/science.aat0271. Epub 2018 May 31.
  PUBMED    29853554
REFERENCE   2  (bases 1 to 11540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aat0271"; journalName: "Science"; date: 
            "2018-07-13- 13"; volume: "361"; issue: "6398"; pages: "156-162"
COMMENT     Sequence Label: pHR_TRE_Ecad
FEATURES             Location/Qualifiers
     source          1..11540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    186..303
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        382..749
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    390..408
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    426..444
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    462..480
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    498..516
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    534..552
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    570..588
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    606..624
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      788..797
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3540..4128
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3593..3613)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4011..4022)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4131..4147)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4132..4148)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4238..4471
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4496..4514)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4497..4514)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4804..4823)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5037..5056)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5156..5178
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5216..5234)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5302..5406
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5407..5475,5476..6267)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5625..5644)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6438..7026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6927..6946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7180..7197
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7267..7287)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7272..7601
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7452..7587
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8735..8800
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8930..8950
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9375..9509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9412..9431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9466..9485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9677..10310
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10357..10482
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10979..11212
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11397..11441
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
       61 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
      121 gacagatcca ttcgattagt gaacggatct cgacggtatc gccaaatggc agtattcatc
      181 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
      241 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
      301 tttcgggttt attacaggga cagcagagat ccagtttgga tcgataagct tgatatcgaa
      361 ttcccgtgga attatcacct cgagtttact ccctatcagt gatagagaac gtatgaagag
      421 tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga
      481 acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat
      541 cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag
      601 tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc agggcgccta
      661 taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt cttataccaa
      721 ctttccgtac cacttcctac cctcgtaaag aattcccgta tacaccggtg caattctcac
      781 gcgtgccgcc accatgggcc cttggagccg cagcctctcg gcgctgctgc tgctgctgca
      841 ggtctcctct tggctctgcc aggagccgga gccctgccac cctggctttg acgccgagag
      901 ctacacgttc acggtgcccc ggcgccacct ggagagaggc cgcgtcctgg gcagagtgaa
      961 ttttgaagat tgcaccggtc gacaaaggac agcctatttt tccctcgaca cccgattcaa
     1021 agtgggcaca gatggtgtga ttacagtcaa aaggcctcta cggtttcata acccacagat
     1081 ccatttcttg gtctacgcct gggactccac ctacagaaag ttttccacca aagtcacgct
     1141 gaatacagtg gggcaccacc accgcccccc gccccatcag gcctccgttt ctggaatcca
     1201 agcagaattg ctcacatttc ccaactcctc tcctggcctc agaagacaga agagagactg
     1261 ggttattcct cccatcagct gcccagaaaa tgaaaaaggc ccatttccta aaaacctggt
     1321 tcagatcaaa tccaacaaag acaaagaagg caaggttttc tacagcatca ctggccaagg
     1381 agctgacaca ccccctgttg gtgtctttat tattgaaaga gaaacaggat ggctgaaggt
     1441 gacagagcct ctggatagag aacgcattgc cacatacact ctcttctctc acgctgtgtc
     1501 atccaacggg aatgcagttg aggatccaat ggagattttg atcacggtaa ccgatcagaa
     1561 tgacaacaag cccgaattca cccaggaggt ctttaagggg tctgtcatgg aaggtgctct
     1621 tccaggaacc tctgtgatgg aggtcacagc cacagacgcg gacgatgatg tgaacaccta
     1681 caatgccgcc atcgcttaca ccatcctcag ccaagatcct gagctccctg acaaaaatat
     1741 gttcaccatt aacaggaaca caggagtcat cagtgtggtc accactgggc tggaccgaga
     1801 gagtttccct acgtataccc tggtggttca agctgctgac cttcaaggtg aggggttaag
     1861 cacaacagca acagctgtga tcacagtcac tgacaccaac gataatcctc cgatcttcaa
     1921 tcccaccacg tacaagggtc aggtgcctga gaacgaggct aacgtcgtaa tcaccacact
     1981 gaaagtgact gatgctgatg cccccaatac cccagcgtgg gaggctgtat acaccatatt
     2041 gaatgatgat ggtggacaat ttgtcgtcac cacaaatcca gtgaacaacg atggcatttt
     2101 gaaaacagca aagggcttgg attttgaggc caagcagcag tacattctac acgtagcagt
     2161 gacgaatgtg gtaccttttg aggtctctct caccacctcc acagccaccg tcaccgtgga
     2221 tgtgctggat gtgaatgaag cccccatctt tgtgcctcct gaaaagagag tggaagtgtc
     2281 cgaggacttt ggcgtgggcc aggaaatcac atcctacact gcccaggagc cagacacatt
     2341 tatggaacag aaaataacat atcggatttg gagagacact gccaactggc tggagattaa
     2401 tccggacact ggtgccattt ccactcgggc tgagctggac agggaggatt ttgagcacgt
     2461 gaagaacagc acgtacacag ccctaatcat agctacagac aatggttctc cagttgctac
     2521 tggaacaggg acacttctgc tgatcctgtc tgatgtgaat gacaacgccc ccataccaga
     2581 acctcgaact atattcttct gtgagaggaa tccaaagcct caggtcataa acatcattga
     2641 tgcagacctt cctcccaata catctccctt cacagcagaa ctaacacacg gggcgagtgc
     2701 caactggacc attcagtaca acgacccaac ccaagaatct atcattttga agccaaagat
     2761 ggccttagag gtgggtgact acaaaatcaa tctcaagctc atggataacc agaataaaga
     2821 ccaagtgacc accttagagg tcagcgtgtg tgactgtgaa ggggccgccg gcgtctgtag
     2881 gaaggcacag cctgtcgaag caggattgca aattcctgcc attctgggga ttcttggagg
     2941 aattcttgct ttgctaattc tgattctgct gctcttgctg tttcttcgga ggagagcggt
     3001 ggtcaaagag cccttactgc ccccagagga tgacacccgg gacaacgttt attactatga
     3061 tgaagaagga ggcggagaag aggaccagga ctttgacttg agccagctgc acaggggcct
     3121 ggacgctcgg cctgaagtga ctcgtaacga cgttgcacca accctcatga gtgtcccccg
     3181 gtatcttccc cgccctgcca atcccgatga aattggaaat tttattgatg aaaatctgaa
     3241 agcggctgat actgacccca cagccccgcc ttatgattct ctgctcgtgt ttgactatga
     3301 aggaagcggt tccgaagctg ctagtctgag ctccctgaac tcctcagagt cagacaaaga
     3361 ccaggactat gactacttga acgaatgggg caatcgcttc aagaagctgg ctgacatgta
     3421 cggaggcggc gaggacgact aggcggccgc aactcccacc tgcaacatgc gtgactgact
     3481 gaggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata
     3541 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3601 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3661 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3721 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3781 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3841 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3901 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3961 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4021 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4081 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     4141 gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta caaggcagct
     4201 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4261 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     4321 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4381 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4441 cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat
     4501 agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt
     4561 ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat
     4621 catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg
     4681 gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc
     4741 agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     4801 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     4861 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     4921 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     4981 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca
     5041 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     5101 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     5161 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     5221 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5281 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5341 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5401 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5461 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5521 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5581 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5641 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5701 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5761 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5821 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5881 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5941 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     6001 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6061 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6121 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6181 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6241 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6301 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6361 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6421 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6481 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6541 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6601 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6661 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6721 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6781 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6841 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6901 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6961 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7021 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7081 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7141 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7201 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7261 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7321 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7381 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7441 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7501 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7561 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag
     7621 gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
     7681 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
     7741 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
     7801 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
     7861 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
     7921 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
     7981 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
     8041 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
     8101 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
     8161 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
     8221 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
     8281 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
     8341 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
     8401 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
     8461 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
     8521 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
     8581 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
     8641 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     8701 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     8761 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     8821 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     8881 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     8941 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     9001 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     9061 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     9121 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     9181 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     9241 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     9301 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     9361 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9421 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9481 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     9541 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     9601 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     9661 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     9721 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     9781 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     9841 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     9901 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     9961 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
    10021 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
    10081 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
    10141 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    10201 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    10261 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
    10321 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
    10381 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
    10441 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
    10501 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
    10561 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    10621 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    10681 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    10741 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    10801 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
    10861 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
    10921 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
    10981 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
    11041 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
    11101 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
    11161 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
    11221 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
    11281 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
    11341 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
    11401 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
    11461 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
    11521 gaatagtttt tgctgtactt
//
