LOCUS       sequence-264959-       12080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of mCherry and Pcad in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Toda S, Blauch LR, Tang SKY, Morsut L, Lim WA
  TITLE     Programming self-organizing multicellular structures with synthetic 
            cell-cell signaling.
  JOURNAL   Science. 2018 Jul 13;361(6398):156-162. doi: 
            10.1126/science.aat0271. Epub 2018 May 31.
  PUBMED    29853554
REFERENCE   2  (bases 1 to 12080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aat0271"; journalName: "Science"; date: 
            "2018-07-13- 13"; volume: "361"; issue: "6398"; pages: "156-162"
COMMENT     Sequence Label: pHR_TRE_mcherry-P2A-Pcad
FEATURES             Location/Qualifiers
     source          1..12080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      343..352
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             349..1056
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(494..512)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     762..781
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1072..1128
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    3635..4223
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3688..3708)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4106..4117)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4226..4242)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4227..4243)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4333..4566
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4591..4609)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4592..4609)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4899..4918)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5132..5151)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5251..5273
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5311..5329)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5397..5501
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5502..5570,5571..6362)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5720..5739)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6533..7121
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7022..7041
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7275..7292
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7362..7382)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7367..7696
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7547..7682
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8830..8895
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9025..9045
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9470..9604
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9507..9526)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9561..9580
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9772..10405
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10452..10577
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11074..11307
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11492..11536
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11821..11938
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(12017..12080,1..304)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    12025..12043
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12061..12079
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 cgtatgcaga ctttactccc tatcagtgat agagaacgta taaggagttt actccctatc
       61 agtgatagag aacgtatgac cagtttactc cctatcagtg atagagaacg tatctacagt
      121 ttactcccta tcagtgatag agaacgtata tccagtttac tccctatcag tgatagagaa
      181 cgtataagct ttgcttatgt aaaccagggc gcctataaaa gagtgctgat tttttgagta
      241 aacttcaatt ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg
      301 taaagaattc ccgtatacac cggtgcaatt ctcacgcgtg ccgccaccat ggtgagcaag
      361 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      421 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      481 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
      541 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
      601 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      661 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
      721 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
      781 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
      841 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
      901 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
      961 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     1021 cgccactcca ccggcggcat ggacgagctg tacaaggcca gcggcagcgg cgccacaaac
     1081 ttctctctgc taaagcaagc aggtgatgtt gaagaaaacc ccgggcctat ggagcttctt
     1141 agtgggcctc acgccttcct gctcctcctg ctccaggttt gctggctacg cagcgtggtc
     1201 tccgagccct accgagcggg cttcatcggg gaggctggag tgaccttgga ggtggaagga
     1261 actgacctgg agccgagcca agttctgggg aaagtagcct tggctggaca gggcatgcac
     1321 catgcagaca atggagacat cattatgctg actaggggga cagttcaggg agggaaggat
     1381 gcgatgcact ccccacccac ccgcatctta aggagacgaa agagagagtg ggtgatgcca
     1441 ccaatattcg tccccgagaa tggcaagggt cccttccctc agaggctgaa tcagctcaaa
     1501 tctaataagg acagaggcac caagattttc tacagcatca cagggcctgg cgcagacagt
     1561 ccccccgaag gagtcttcac catagagaag gagtcgggct ggctgttgtt gcatatgcca
     1621 ctggacaggg agaagattgt caagtacgag ctttatggcc acgctgtatc tgagaatggt
     1681 gcctctgtag aggagcccat gaacatatcc atcattgtga cagaccagaa tgacaacaag
     1741 cccaagttca ctcaagacac cttcagaggg agtgttctgg agggagtaat gcctggcact
     1801 tctgtgatgc aggtgacagc cacagatgag gacgatgctg tcaacactta caatggggtg
     1861 gtggcttact ccatccatag ccaagagccg aaggagccac acgacctcat gttcaccatc
     1921 cataaaagca cgggaaccat tagcgtcata tccagtggcc tggaccgaga gaaagtccct
     1981 gagtacagac tgaccgtcca ggccacagac atggatggag agggctctac cacgacggca
     2041 gaggccgttg tgcaaatcct tgatgccaac gataacgctc cggagtttga gccgcagaag
     2101 tatgaggctt gggtgcctga gaacgaagtg ggccatgagg tacagaggct gacagtgact
     2161 gatctcgatg tccccaactc gccagcgtgg cgtgccacct accacatcgt gggaggtgat
     2221 gatggggacc atttcaccat caccactcac ccagagacca accaaggcgt cctgacaacc
     2281 aagaagggtt tggattttga ggctcaggac caacacaccc tgtatgtaga agtgaccaac
     2341 gaggctccct ttgcagtgaa gctcccgaca gccactgcca ccgtggtggt ccatgtgaaa
     2401 gatgtcaacg aagcccctgt gtttgttcca ccttccaagg tcattgaggc ccaggaaggc
     2461 atctctattg gggaactggt ctgcatctat accgcacagg acccagacaa ggaggaccag
     2521 aagatcagct acaccatctc gagagatcca gccaactggc ttgctgtgga cccagacagt
     2581 ggtcagataa ctgccgcagg catcttggat cgtgaggacg agcagtttgt gaaaaacaat
     2641 gtctacgaag tcatggtttt ggccacagac agtggaaacc ctcccaccac cggcactggg
     2701 accctcctgc ttacacttac tgacatcaac gaccatggcc cgatccctga acccaggcag
     2761 atcatcatct gtaaccaaag ccctgtgcct caagtgctga acatcactga caaggacctg
     2821 tcccccaact cctccccttt ccaggcccag ctaacacatg actcagatat ctactggatg
     2881 gcagaagtca gcgagaaagg agacaccgtg gccttgtccc tgaagaagtt cctgaaacaa
     2941 gacacgtatg acttgcatct ttctttgtct gaccatggca acagggaaca gctaaccatg
     3001 atcagggcca ctgtgtgtga ctgccatggc caagtgttca atgactgccc cagaccctgg
     3061 aagggtggtt tcatcctccc catcctgggt gctgtcctgg cactgctgac ccttctactg
     3121 gcactcctcc tgttggtgag gaagaagagg aaggtcaaag agccccttct gctcccagaa
     3181 gatgacacgc gagacaatgt cttctattat ggagaagagg gtggtggtga agaggaccag
     3241 gactatgaca tcacccaact ccaccgggga ctggaggcca ggcctgaggt ggttctccga
     3301 aacgatgtag tgccaacctt catccccacc cccatgtacc gaccccggcc cgccaaccca
     3361 gatgaaatcg ggaacttcat catcgagaac ctgaaggctg ccaacactga ccctactgcc
     3421 ccgccctacg actccctgct ggtttttgac tacgagggca gcggctctga tgccgcctcc
     3481 ctgagctccc tcaccacctc cgcctccgac caggatcagg actacaacta ccttaacgag
     3541 tggggaagtc gattcaagaa actggcggac atgtatggtg gcggtgagga tgactagcct
     3601 gcaggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     3661 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3721 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3781 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3841 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3901 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3961 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4021 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     4081 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4141 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4201 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct
     4261 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4321 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     4381 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4441 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4501 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4561 ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat
     4621 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa
     4681 ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag
     4741 agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat
     4801 ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt
     4861 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     4921 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     4981 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     5041 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     5101 taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     5161 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5221 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5281 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     5341 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     5401 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5461 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5521 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5581 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5641 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5701 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5761 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5821 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5881 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5941 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6001 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6061 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6121 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6181 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6241 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6301 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6361 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6421 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6481 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6541 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6601 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6661 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6721 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6781 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6841 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6901 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6961 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7021 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7081 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7141 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7201 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7261 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7321 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
     7381 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7441 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7501 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     7561 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7621 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7681 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
     7741 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     7801 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     7861 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     7921 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     7981 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
     8041 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     8101 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     8161 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     8221 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     8281 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     8341 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     8401 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     8461 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     8521 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     8581 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     8641 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     8701 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
     8761 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     8821 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     8881 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     8941 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     9001 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     9061 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     9121 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     9181 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     9241 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     9301 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     9361 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     9421 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     9481 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9541 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9601 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     9661 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     9721 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
     9781 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     9841 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     9901 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     9961 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
    10021 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
    10081 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
    10141 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
    10201 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
    10261 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    10321 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10381 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
    10441 aaccagagga gctctctcga cgcaggactc ggcttggtga agcgcgcacg gcaagaggcg
    10501 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
    10561 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
    10621 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
    10681 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
    10741 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
    10801 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
    10861 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
    10921 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
    10981 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    11041 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    11101 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    11161 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    11221 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    11281 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    11341 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    11401 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    11461 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    11521 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    11581 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    11641 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    11701 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
    11761 atccattcga ttagtgaacg gatctcgacg gtatcgccaa atggcagtat tcatccacaa
    11821 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
    11881 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
    11941 ggtttattac agggacagca gagatccagt ttggatcgat aagcttgata tcgaattccc
    12001 gtggaattat cacctcgagt ttactcccta tcagtgatag agaacgtatg aagagtttac
    12061 tccctatcag tgatagagaa
//
