LOCUS       sequence-264962-       11702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of tTS and GFP with BFP marker in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11702)
  AUTHORS   Toda S, Blauch LR, Tang SKY, Morsut L, Lim WA
  TITLE     Programming self-organizing multicellular structures with synthetic 
            cell-cell signaling.
  JOURNAL   Science. 2018 Jul 13;361(6398):156-162. doi: 
            10.1126/science.aat0271. Epub 2018 May 31.
  PUBMED    29853554
REFERENCE   2  (bases 1 to 11702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aat0271"; journalName: "Science"; date: 
            "2018-07-13- 13"; volume: "361"; issue: "6398"; pages: "156-162"
COMMENT     Sequence Label: pHR_UAS-tTS-P2A-GFP_PGK-BFP
FEATURES             Location/Qualifiers
     source          1..11702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    189..306
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     532..556
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(649..1281,1282..1302,1303..1494)
                     /codon_start=1
                     /product="fusion of a hybrid tetracycline repressor TetR 
                     with a nuclear localization signal and a KRAB silencing 
                     domain"
                     /label=tTS
                     /note="silences Tet-responsive promoters in the absence but
                     not the presence of doxycycline"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     RNKQTLMNMLSEAILAKHHTRSAPLPTESWQQFLQENALSFRKALLVHRDGARLHIGTS
                     PTPPQFEQAEAQLRCLCDAGFSVEEALFILQSISHFTLGAVLEEQATNQIENNHVIDAA
                     PPLLQEAFNIQARTSAEMAFHFGLKSLIFGFSAQLDEKKHTPIEDGNKPKKKRKLAVSV
                     TFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASMAGFLFTKPKVISLLQQGEDP
                     W"
     primer_bind     complement(722..741)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             1510..1566
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1570..1572,1573..1575,1576..2289)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1570..1572,1573..1575,1576..2286)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1615..1636)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1876..1895)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2223..2244
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2305..2327
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2343..2359
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        2410..2909
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2445..2468)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2842..2861
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2930..3631
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    3705..4293
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3758..3778)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4176..4187)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4296..4312)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4297..4313)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4403..4636
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4661..4679)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4662..4679)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4969..4988)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5202..5221)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5321..5343
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5381..5399)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5467..5571
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5572..5640,5641..6432)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5790..5809)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6603..7191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7092..7111
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7345..7362
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7432..7452)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7437..7766
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7617..7752
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8900..8965
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9095..9115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9540..9674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9577..9596)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9631..9650
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9842..10475
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10522..10647
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11144..11377
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11562..11606
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
       61 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
      121 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
      181 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      241 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      301 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
      361 gaattcggag cactgtcctc cgaacgtcgg agcactgtcc tccgaacgtc ggagcactgt
      421 cctccgaacg tcggagcact gtcctccgaa cggagcatgt cctccgaacg tcggagcact
      481 gtcctccgaa cgactagtta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
      541 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
      601 ccgggaccga tccagcctct cgacattcgt tggatcgcgg ccgccataat gtctagatta
      661 gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt
      721 ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcac
      781 gtgcgcaaca agcagactct tatgaacatg ctttcagagg caatactggc gaagcatcac
      841 acccgttcag caccgttacc gactgagagt tggcagcagt ttctccagga aaatgctctg
      901 agtttccgta aagcattact ggtccatcgt gatggagccc gattgcatat agggacctct
      961 cctacgcccc cccagtttga acaagcagag gcgcaactac gctgtctatg cgatgcaggg
     1021 ttttcggtcg aggaggctct tttcattctg caatctatca gccattttac gttgggtgca
     1081 gtattagagg agcaagcaac aaaccagata gaaaataatc atgtgataga cgctgcacca
     1141 ccattattac aagaggcatt taatattcag gcgagaacct ctgctgaaat ggccttccat
     1201 ttcgggctga aatcattaat atttggattt tctgcacagt tagatgaaaa aaagcataca
     1261 cccattgagg atggtaataa accaaaaaag aagagaaagc tagcagtgtc agtgacattt
     1321 gaagatgtgg ctgtgctctt tactcgggac gagtggaaga agctggatct gtctcagaga
     1381 agcctgtacc gtgaggtgat gctggagaat tacagcaacc tggcctccat ggcaggattc
     1441 ctgtttacca aaccaaaggt gatctccctg ttgcagcaag gagaggatcc ctgggccagc
     1501 ggcagcggcg ccacaaactt ctctctgcta aagcaagcag gtgatgttga agaaaacccc
     1561 gggcctcata tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1621 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1681 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1741 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1801 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1861 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1921 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1981 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2041 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2101 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2161 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2221 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2281 tacaagtaag gatcccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct
     2341 ccctttatcc agccctcact ccttctctag gcgccggaat tagatctctc gaggttaacg
     2401 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     2461 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     2521 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     2581 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     2641 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     2701 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     2761 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     2821 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     2881 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgagcgagct
     2941 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
     3001 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     3061 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
     3121 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt
     3181 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     3241 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     3301 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac
     3361 cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct
     3421 cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc
     3481 taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga
     3541 ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct
     3601 ccctagcaaa ctggggcaca agcttaatta aggccgcgac tctagagtcg acctgcaggc
     3661 atgcaagctt gcaggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt
     3721 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3781 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3841 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3901 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3961 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4021 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4081 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     4141 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4201 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4261 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagggaatt
     4321 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     4381 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     4441 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     4501 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     4561 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     4621 tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc
     4681 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
     4741 acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa
     4801 ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc
     4861 acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg
     4921 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     4981 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
     5041 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
     5101 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
     5161 aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc
     5221 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5281 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     5341 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     5401 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5461 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5521 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5581 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5641 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5701 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5761 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5821 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5881 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5941 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6001 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6061 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6121 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6181 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6241 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6301 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6361 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6421 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6481 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6541 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6601 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6661 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6721 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6781 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6841 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6901 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6961 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7021 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7081 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7141 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7201 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7261 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7321 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7381 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt
     7441 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7501 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7561 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7621 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7681 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7741 tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt
     7801 ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca
     7861 tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc
     7921 tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac
     7981 atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct
     8041 tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac
     8101 tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg
     8161 acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg
     8221 ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa
     8281 aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg
     8341 ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg
     8401 tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt
     8461 ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca
     8521 caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg
     8581 ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa
     8641 accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac
     8701 ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac
     8761 gatgatttat acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc
     8821 ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     8881 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     8941 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     9001 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     9061 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     9121 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     9181 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     9241 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     9301 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     9361 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     9421 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     9481 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     9541 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9601 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9661 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
     9721 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
     9781 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
     9841 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
     9901 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     9961 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
    10021 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
    10081 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
    10141 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
    10201 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
    10261 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
    10321 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10381 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10441 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
    10501 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    10561 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    10621 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    10681 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    10741 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    10801 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    10861 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    10921 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    10981 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
    11041 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
    11101 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
    11161 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
    11221 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
    11281 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
    11341 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
    11401 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
    11461 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
    11521 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
    11581 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
    11641 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
    11701 ta
//
