LOCUS       sequence-264971-        3153 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Viral expression of sRGECO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M,
            Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K
  TITLE     Comprehensive Dual- and Triple-Feature Intersectional Single-Vector 
            Delivery of Diverse Functional Payloads to Cells of Behaving 
            Mammals.
  JOURNAL   Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003.
  PUBMED    32574559
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003."
COMMENT     Sequence Label: pAAV-Ef1a-sRGECO
FEATURES             Location/Qualifiers
     source          1..3153
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        87..1264
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          318..1255
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1212..1232
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1271..1280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1292..1348
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     2044..2063
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2555..3143
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2608..2628)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3026..3037)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
       61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
      121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
      181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
      241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
      301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
      361 gttatggccc ttgcgtgcct tgaattactt ccactggctg cagtacgtga ttcttgatcc
      421 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
      481 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
      541 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
      601 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
      661 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
      721 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
      781 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
      841 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
      901 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
      961 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     1021 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     1081 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     1141 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     1201 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     1261 gtgaggatcc gccaccatgg tggactcatc acgtcgtaag tggaataagg caggtcacgc
     1321 agtcagagct ataggtcggc tgagctcacc cgtggtttcc gagcggatgt accccgagga
     1381 cggcgccctg aagagcgaga tcaagaaggg gctgaggctg aaggacggcg gccactacgc
     1441 cgccgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacat
     1501 cgtggacatc aagttggaca tcgtgtccca caacgaggac tacaccatcg tggaacagtg
     1561 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agggaggtac
     1621 aggcgggagt ctggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     1681 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     1741 gggcgagggc cgcccctacg aggcctttca gaccgctaag ctgaaggtga ccaagggtgg
     1801 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     1861 cattaagcac ccagccgaca tccccgacta cttcaagctg tccttccccg agggcttcag
     1921 gtgggaccgc gtgatgaact tcgaggacgg cggcattatt cacgtgaacc aggactcctc
     1981 cctgcaggac ggcgtattca tctacaaggt gaagctgcgc ggcaccaact tcccccccga
     2041 cggccccgta atgcagaaga agaccatggg ctgggaggct acgcgtgacg acctgactga
     2101 agagcagatc gcagaattta aagaggcttt ctccctattt gacaaggacg gggatgggac
     2161 gataacaacc aaggagcttg ggacggtgtt ccgatcgctg gggcagaacc ccacagaagc
     2221 agagctgcag gacatgatca atgaagtaga tgccgacggt gacggcacat tcgacttccc
     2281 tgagttcctg acgatgatgg caagaaaaat gaatgacaca gacagtgaag aggaaattcg
     2341 cgaagcgttc cgcgtgtttg ataaggacgg caatggctac atcggcgcag cagagcttcg
     2401 ccacgtgatg acagaccttg gagagaagtt aacagatgag gaggttgatg aaatgatcag
     2461 ggtagcagac atcgatgggg atggtcaggt aaactacgaa gagtttgtac aaatgatgac
     2521 agcgaagtag gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     2581 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2641 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2701 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2761 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2821 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2881 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2941 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg
     3001 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3061 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3121 ctccctttgg gccgcctccc cgcatcgata ccg
//
