LOCUS       sequence-264989-        3531 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intersectional viral expression of ChR2-mCherry in cells expressing 
            Cre AND NOT Flp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M,
            Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K
  TITLE     Comprehensive Dual- and Triple-Feature Intersectional Single-Vector 
            Delivery of Diverse Functional Payloads to Cells of Behaving 
            Mammals.
  JOURNAL   Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003.
  PUBMED    32574559
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003."
COMMENT     Sequence Label: pAAV-nEF-Con/Foff 2.0-ChR2-mCherry
FEATURES             Location/Qualifiers
     source          1..3531
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..244
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             257..525
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     protein_bind    553..586
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      679..688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1124..1157)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(1208..1241)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     1440..1458
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    2224..2257
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    2308..2341
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     2487..2506
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2791..2824)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    2933..3521
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2986..3006)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3404..3415)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
       61 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
      121 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
      181 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
      241 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
      301 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
      361 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
      421 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
      481 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
      541 gcctacggat ccgaagttcc tattctctag aaagtatagg aacttcgcag agtagtagct
      601 ggattgtagc tgctattagc aatctaaaac ctcttagaag ttcctattct tcaaaaggta
      661 taggaacttc ggcgcgccgc caccatggac tatggcggcg ctttgtctgc cgtcggacgc
      721 gaacttttgt tcgttactaa tcctgtggtg gtgaacgggt ccgtcctggt ccctgaggat
      781 caatgttact gtgccggatg gattgaatct cgcggcacga acggcgctca gaccgcgtca
      841 aatgtcctgc agtggcttgc agcaggattc agcattttgc tgctgatgtt ctatgcctac
      901 caaacctgga aatctacatg cggctgggag gagatctatg tgtgcgccat tgaaatggtt
      961 aaggtgattc tcgagttctt ttttgagttt aagaatccct ctatgctcta ccttgccaca
     1021 ggacaccggg tgcagtggct gcgctatgca gagtggctgc tcacttgtcc tgtcatcctt
     1081 atccgcctga gcaacctcac cggtaagtgt cggggttgct agcataactt cgtatagtat
     1141 accttatacg aagttattct ttgcaccatt ctaaagaata acagtgataa tttctgggtt
     1201 aaggcaaata acttcgtata ggatacttta tacgaagtta tggtacccct cctgtactca
     1261 cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc
     1321 cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct
     1381 tggagccgta catgaactga ggggacagga tgtcccaggc gaagggcagg gggccaccct
     1441 tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct
     1501 cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag cgcatgaact
     1561 ccttgatgat ggccatgtta tcctcctcgc ccttgctcac ggcggccgct ggcacggctc
     1621 cggcctcggc ttcgtcttcg acgagagtct cgacctcgat ctccgttccg ccgatgttca
     1681 gtttggtggt tttgcggata tctccgtgaa tcaatatgtg ctcgtggatc aggacgcgca
     1741 ggtagtgtcc caacaacccc caacaatttt tactcatcag atcaataatc gtgtgaccta
     1801 cggtggagcc atagacgctc aggacgccaa aaccttcggg ccccaaaatg aagagaattg
     1861 ggaacatacc ccagctcacg aaaaacagcc atgccatgcc ggtcacgacc tggcggcacc
     1921 gaccctttgg cacagtatga taaccctcga tatatgcttt ggcggcgtga aaaaatgtgt
     1981 tcgcgccata gcacaatcca agacaaaaga agatgacttt aacatagccg gttgccatgg
     2041 cgctggtagc cccccacacg atagtcccga tgtctgagac aaggagtccc atggttctcc
     2101 tgctgtagtc gttgctcagg cctaaacaca tgagaaatag agtcataagc acatgggtca
     2161 catacaggag atatgtatat aacattaata caatttataa aaaaaagggg gggcacaact
     2221 agtataactt cgtataaggt atactatacg aagttattaa gaggtttcat attgctaata
     2281 gcagctacaa tccagctacc attctgcata acttcgtata aagtatccta tacgaagtta
     2341 tgtcgactgg agagtggcca gcccttctca tgttcagaga acatggttaa ctggttaagt
     2401 catgtcgtcc cacaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     2461 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     2521 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     2581 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     2641 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     2701 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     2761 ggcatggacg agctgtacaa gtaagctagc gaagttccta tactttctag agaataggaa
     2821 cttctaagca gacaatcttg taatacatca gcagctagta gattgctcca gctagaagtt
     2881 cctatacctt ttgaagaata ggaacttcga attcgatatc aagcttatcg ataatcaacc
     2941 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3001 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3061 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     3121 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     3181 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     3241 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     3301 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     3361 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     3421 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     3481 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc g
//
