LOCUS       sequence-26500-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Hackett EA, Esch RK, Maleri S, Errede B
  TITLE     A family of destabilized cyan fluorescent proteins as 
            transcriptional reporters in S. cerevisiae.
  JOURNAL   Yeast. 2006 Apr 15. 23(5):333-49.
  PUBMED    16598699
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Apr 15. 23(5):333-49."
COMMENT     Sequence Label: pNC820
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..234)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESXLHLVLRLRGG"
     primer_bind     complement(707..723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(707..723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(720..742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    731..747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(755..761,762..779,780..785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    800..821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtgcatacca cctcttagcc ttagcacaag atgtaaggng gactccttct gaatgttgta
       61 atcagacagc gttctaccgt cttctagctg cttaccggca aagatcaatc tttgttgatc
      121 tggagggata ccttccttgt cttgaatttt cgacttaacg ttgtcgatgg tatcggaaga
      181 ttcaacttcc aatgttatgg ttttaccggt caaagtcttg acgaaaatct gcataatcta
      241 ttagttaaag taaagtggga gggagagttc gagagttctt ttcgtattgt agtagataga
      301 agatatactt atccttatcc gtacagttct tgctctttct gaagtagcac tttcggaaga
      361 agaaagataa gcaggcgaat tgatccggta atttagtgtg tgtatttgtg tttgcgtgtc
      421 tatagaagta tagtaattta tgctacaaag gacctaatgt ataaggaaag aatatttaga
      481 gaaaagaaga aaacaagagt tttatataca tacagagcac atgcatgcca tatgatcatg
      541 tgtcgtcgca cacatatata tatgcctgta tgtgtcagca ctaaagttgc ctggccatcc
      601 acgctatata cacgcctggc agatctgctc gatttgtttc aaattacaga cgtttcatgt
      661 ttgtttcatc actcgagggg ggtaccgagc tcgaattcgt aatcatgtca tagctgtttc
      721 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      781 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      841 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      901 ggagaggcgg tttgcgtatt gggcg
//
