LOCUS       sequence-265004-        2987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Viral expression of ChRmine-p2a-mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M,
            Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K
  TITLE     Comprehensive Dual- and Triple-Feature Intersectional Single-Vector 
            Delivery of Diverse Functional Payloads to Cells of Behaving 
            Mammals.
  JOURNAL   Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003.
  PUBMED    32574559
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003."
COMMENT     Sequence Label: pAAV-nEF-ChRmine-mScarlet
FEATURES             Location/Qualifiers
     source          1..2987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..244
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             257..525
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      565..574
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1585..1641
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1642..2337
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1775..1793)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2043..2062
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2389..2977
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2442..2462)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2860..2871)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
       61 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
      121 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
      181 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
      241 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
      301 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
      361 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
      421 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
      481 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
      541 gcctacggat ccccgggtac cggtgccacc atggcacacg caccaggcac cgaccagatg
      601 ttctacgtgg gcacaatgga cggctggtat ctggatacca agctgaactc cgtggccatc
      661 ggcgcccact ggtcttgctt tatcgtgctg acaatcacca cattctacct gggctatgag
      721 tcctggacat ctcggggccc tagcaagaga acctcctttt acgccggcta tcaggaggag
      781 cagaacctgg ccctgtttgt gaatttcttt gccatgctga gctacttcgg caagatcgtg
      841 gccgacacac tgggccacaa cttcggcgat gtgggcccct tcatcatcgg cttcggcaat
      901 tacaggtatg ccgactacat gctgacctgc ccaatgctgg tgtacgatct gctgtatcag
      961 ctgagggccc cctatcgcgt gtcttgtagc gccatcatct ttgccatcct gatgtctggc
     1021 gtgctggccg agttctacgc agagggcgac cctaggctga ggaatggcgc ctacgcctgg
     1081 tatggctttg gctgtttctg gtttatcttc gcctactcta tcgtgatgag catcgtggcc
     1141 aagcagtata gccggctggc ccagctggcc caggatacag gcgccgagca cagcctgcac
     1201 gtgctgaagt tcgccgtgtt taccttctcc atgctgtgga ttctgtttcc cctggtgtgg
     1261 gccatctgcc ctagaggctt cggctggatc gacgataact ggacagaggt ggcccactgc
     1321 gtgtgcgaca tcgtggccaa gtcctgttac ggctttgccc tggcccggtt cagaaagacc
     1381 tatgatgagg agctgtttcg gctgctggag cagctgggac acgacgagga tgagttccag
     1441 aagctggagc tggatatgag gctgagcagc aatggcgagc gcctgcggag actgtctgcg
     1501 gccgccaaga gcaggatcac cagcgagggc gagtacatcc ccctggacca gatcgacatc
     1561 aacgtgggcg cgcccggctc cggagccacg aacttctctc tgttaaagca agcaggagac
     1621 gtggaagaaa accccggtcc catggtgagc aagggcgagg cagtgatcaa ggagttcatg
     1681 cggttcaagg tgcacatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     1741 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     1801 cccctgccct tctcctggga catcctgtcc cctcagttca tgtacggctc cagggccttc
     1861 accaagcacc ccgccgacat ccccgactac tataagcagt ccttccccga gggcttcaag
     1921 tgggagcgcg tgatgaactt cgaggacggc ggcgccgtga ccgtgaccca ggacacctcc
     1981 ctggaggacg gcaccctgat ctacaaggtg aagctccgcg gcaccaactt ccctcctgac
     2041 ggccccgtaa tgcagaagaa gacaatgggc tgggaagcgt ccaccgagcg gttgtacccc
     2101 gaggacggcg tgctgaaggg cgacattaag atggccctgc gcctgaagga cggcgggcgc
     2161 tacctggcgg acttcaagac cacctacaag gccaagaagc ccgtgcagat gcccggcgcc
     2221 tacaacgtgg accgcaagtt ggacatcacc tcccacaacg aggactacac cgtggtggaa
     2281 cagtacgaac gctccgaggg ccgccactcc accggcggca tggacgagct gtacaagttc
     2341 tgctacgaga acgaggtgta atgagaattc gatatcaagc ttatcgataa tcaacctctg
     2401 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2461 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2521 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2581 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     2641 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     2701 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     2761 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc
     2821 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     2881 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     2941 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccg
//
