LOCUS       sequence-265007-        3679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intersectional viral expression of ChRmine-p2a-oScarlet in cells 
            expressing Cre AND NOT Flp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M,
            Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K
  TITLE     Comprehensive Dual- and Triple-Feature Intersectional Single-Vector 
            Delivery of Diverse Functional Payloads to Cells of Behaving 
            Mammals.
  JOURNAL   Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003.
  PUBMED    32574559
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003."
COMMENT     Sequence Label: pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet
FEATURES             Location/Qualifiers
     source          1..3679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..244
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             257..525
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     protein_bind    553..586
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      679..688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1041..1074)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(1125..1158)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     1239..1257
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(1391..1447)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    2230..2263
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    2314..2347
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     2611..2630
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2939..2972)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    3081..3669
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3134..3154)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3552..3563)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
       61 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
      121 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
      181 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
      241 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
      301 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
      361 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
      421 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
      481 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
      541 gcctacggat ccgaagttcc tattctctag aaagtatagg aacttcgcag agtagtagct
      601 ggattgtagc tgctattagc aatctaaaac ctcttagaag ttcctattct tcaaaaggta
      661 taggaacttc ggcgcgccgc caccatggca cacgcaccag gcaccgacca gatgttctac
      721 gtgggcacaa tggacggctg gtatctggat accaagctga actccgtggc catcggcgcc
      781 cactggtctt gctttatcgt gctgacaatc accacattct acctgggcta tgagtcctgg
      841 acatctcggg gccctagcaa gagaacctcc ttttacgccg gctatcagga ggagcagaac
      901 ctggccctgt ttgtgaattt ctttgccatg ctgagctact tcggcaagat cgtggccgac
      961 acactgggcc acaacttcgg cgatgtgggc cccttcatca tcggcttcgg caattacagg
     1021 taagtgtcgg ggttgagctc ataacttcgt atagtatacc ttatacgaag ttattctttg
     1081 caccattcta aagaataaca gtgataattt ctgggttaag gcaaataact tcgtatagga
     1141 tactttatac gaagttatgg tacccctcct gtactcacct ggagccgtac atgaactgag
     1201 gggacaggat gtcccaggag aagggcaggg ggccaccctt ggtcaccttc agcttggcgg
     1261 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
     1321 tcatggagcc ctccatgtgc accttgaacc gcatgaactc cttgatcact gcctcgccct
     1381 tgctcaccat gggaccgggg ttttcttcca cgtctcctgc ttgctttaac agagagaagt
     1441 tcgtggctcc ggagccgggc gcgcccacgt tgatgtcgat ctggtccagg gggatgtact
     1501 cgccctcgct ggtgatcctg ctcttggcgg ccgcagacag tctccgcagg cgctcgccat
     1561 tgctgctcag cctcatatcc agctccagct tctggaactc atcctcgtcg tgtcccagct
     1621 gctccagcag ccgaaacagc tcctcatcat aggtctttct gaaccgggcc agggcaaagc
     1681 cgtaacagga cttggccacg atgtcgcaca cgcagtgggc cacctctgtc cagttatcgt
     1741 cgatccagcc gaagcctcta gggcagatgg cccacaccag gggaaacaga atccacagca
     1801 tggagaaggt aaacacggcg aacttcagca cgtgcaggct gtgctcggcg cctgtatcct
     1861 gggccagctg ggccagccgg ctatactgct tggccacgat gctcatcacg atagagtagg
     1921 cgaagataaa ccagaaacag ccaaagccat accaggcgta ggcgccattc ctcagcctag
     1981 ggtcgccctc tgcgtagaac tcggccagca cgccagacat caggatggca aagatgatgg
     2041 cgctacaaga cacgcgatag ggggccctca gctgatacag cagatcgtac accagcattg
     2101 ggcaggtcag catgtagtcg gcatacctaa acacatgaga aatagagtca taagcacatg
     2161 ggtcacatac aggagatatg tatataacat taatacaatt ttataaaaaa aagggggggc
     2221 acaactagta taacttcgta taaggtatac tatacgaagt tattaagagg tttcatattg
     2281 ctaatagcag ctacaatcca gctaccattc tgcataactt cgtataaagt atcctatacg
     2341 aagttatgtc gactggagag tggccagccc ttctcatgtt cagagaacat ggttaactgg
     2401 ttaagtcatg tcgtcccaca gggccttcac caagcacccc gccgacatcc ccgactacta
     2461 taagcagtcc ttccccgagg gcttcaagtg ggaccgcgtg atgaacttcg aggacggcgg
     2521 cgccgtgacc gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa
     2581 gctccgcggc accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg
     2641 ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat
     2701 ggccctgcgc ctgaaggacg gcgggcgcta cctggcggac ttcaagacca cctacaaggc
     2761 caagaagccc gtgcagatgc ccggcgccta caacgtggac cgcaagttgg acatcacctc
     2821 ccacaacgag gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac
     2881 cggcggcatg gacgagctgt acaagttctg ctacgagaac gaggtgtaat gagctagcga
     2941 agttcctata ctttctagag aataggaact tctaagcaga caatcttgta atacatcagc
     3001 agctagtaga ttgctccagc tagaagttcc tatacctttt gaagaatagg aacttcgaat
     3061 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     3121 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3181 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3241 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     3301 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     3361 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     3421 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     3481 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     3541 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3601 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3661 cctccccgca tcgataccg
//
