LOCUS       sequence-265010-        2890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intersectional viral expression of EYFP in cells expressing Cre AND 
            Flp AND Vcre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M,
            Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K
  TITLE     Comprehensive Dual- and Triple-Feature Intersectional Single-Vector 
            Delivery of Diverse Functional Payloads to Cells of Behaving 
            Mammals.
  JOURNAL   Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003.
  PUBMED    32574559
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003."
COMMENT     Sequence Label: pAAV-nEF-Con/Fon/Von eYFP
FEATURES             Location/Qualifiers
     source          1..2890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..244
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             257..525
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     protein_bind    complement(553..586)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(637..670)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     786..807
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      849..858
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    865..898
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    949..982
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    1078..1111
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(1385..1418)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     CDS             complement(1695..1946)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(1737..1758)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2086..2105
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2292..2880
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2345..2365)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2763..2774)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
       61 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
      121 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
      181 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
      241 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
      301 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
      361 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
      421 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
      481 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
      541 gcctacggat ccataacttc gtataggata ctttatacga agttatgtag ctggagcaat
      601 ttgaaaagat tgttgtatta cctctagctg cgcttaataa cttcgtatag tataccttat
      661 acgaagttat ggcgcgccaa ccccgacact taccttgccg gtggtgcaga tgaacttcag
      721 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
      781 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
      841 cttgctcacc atggtggcac cggtataact tcgtataaag tatcctatac gaagttattt
      901 gccttagtta ttcttagata gacccagaaa ttatcacttg tgcaaagaat aacttcgtat
      961 aaggtatact atacgaagtt atgagctctg tgccccccct tttttttata aaattgtatt
     1021 aatgttatat acatatctcc tgtatgtgac ccatgtgctt atgactctat taattaagaa
     1081 gttcctattc ttcaaatagt ataggaactt cgcagaatgg tagctggatt gtagctgcta
     1141 ttagcaatat gaaacctctt agaagttcct attcttcaaa aggtatagga acttcactag
     1201 tcctcctgta ctcaccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
     1261 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc actgcaggcc
     1321 gtagccgaag gtggtcacga gggtgggcca gggcacgggc agctaaacac atgagaaagt
     1381 cgacgaagtt cctatactat ttgaagaata ggaacttctt gccttaaccc agaaattatc
     1441 actgttattc tttagaatgg tgcaaagaga agttcctata ccttttgaag aataggaact
     1501 tcgctagctg gagagtggcc agcccttctc atgttcagag aacatggtta actggttaag
     1561 tcatgtctca atttctgaga actgtcattc tcggaaattg agtagctggt tgaagattgc
     1621 tctagtatta cagcaatttg aactgcgctt atcaatttct gagaagtgtc tttctcggaa
     1681 attgaggtac cttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
     1741 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
     1801 tagctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
     1861 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
     1921 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
     1981 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
     2041 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
     2101 tcgtcctgtg ggatctagag tcaagattgc tccagcaatc ttgtaatact caatttccga
     2161 gaatgacagt tctcagaaat tgataagcag acaatcttgt aatacatgca gctagtcaag
     2221 attgctccag ctatcaattt ccgagaaaga cacttctcag aaattgagaa ttcgatatca
     2281 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2341 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2401 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2461 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2521 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2581 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2641 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2701 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2761 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2821 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     2881 atcgataccg
//
