LOCUS       sequence-265014-        9517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid includes a miRFP703 gene driven by a minimal CMV 
            promoter. Cis-regulatory elements can be cloned into the single 
            EcoRV site. This plasmid can be used for generating a lentiviral 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Roman Azcona MS, Fang Y, Carusillo A, Cathomen T, Mussolino C
  TITLE     A versatile reporter system for multiplexed screening of effective 
            epigenome editors.
  JOURNAL   Nat Protoc. 2020 Oct;15(10):3410-3440. doi: 
            10.1038/s41596-020-0380-y. Epub 2020 Sep 4.
  PUBMED    32887975
REFERENCE   2  (bases 1 to 9517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41596-020-0380-y"; journalName: "Nat Protoc"; date: 
            "2020-10"; volume: "15"; issue: "10"; pages: "3410-3440"
COMMENT     Sequence Label: pCCl-MCS_miniCMV_miRFP703
FEATURES             Location/Qualifiers
     source          1..9517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             219..399
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    446..571
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1064..1297
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1482..1526
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1793..1910
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    2047..2181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3328..3366)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        3394..3904
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3823..3841
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    4908..5496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4961..4981)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5379..5390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5583..5816
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5888..6009
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6049..6184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6204..6223)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6205..6223)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6233..6250)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6233..6249)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6242..6264)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6391..6846
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6478..6497)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6688..6709
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6872..6976
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6977..7045,7046..7837)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7195..7214)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8008..8596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8497..8516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8750..8767
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8884..8905
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8920..8925,8926..8943,8944..8950)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8958..8974
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8963..8985
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8982..8998
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8982..8998
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9017..9037
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9019..9037
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        9138..9517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccggg gtctctctgg ttagaccaga
      241 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      301 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      361 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      421 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
      481 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
      541 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
      601 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
      661 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
      721 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
      781 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
      841 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
      901 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
      961 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1021 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1081 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1141 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1201 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1261 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1321 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1381 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1441 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1501 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1561 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1621 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1681 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     1741 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     1801 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     1861 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg
     1921 agactagcct cgatagatct gatcataatc agccatacca catttgtaga ggttttactt
     1981 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     2041 gttgttaact tgtttattgc agcttataat ggttacaaat aaggcaatag catcacaaat
     2101 ttcacaaata aggcattttt ttcactgcat tctagttttg gtttgtccaa actcatcaat
     2161 gtatcttatc atgtctggat ctcaaatccc tcggaagctg cgcctgtcat cgaattcctg
     2221 cagcccggtg catgactaag ctagctcagt tagcctcccc catctccctc gacgcggccg
     2281 cttcatgatt cgagtgctgt tatcctagtt gcaatgcgtt ccgccaaccg tttgcaaata
     2341 gccctcagct caaacctaat gaagcgggga aggtaatgat ggcacaccac caggccccag
     2401 agcttacccc caacaacaag ggacactgcc aacgtagcgc gtactcccat atctttgagg
     2461 aattgcaggt ggatgggact catacttctc aggaaacacc cgctcatatc caggtctcgc
     2521 cccgtcagcg gagacaggcg tggctccagg gggacaggtt ggtaagtaac atctaccaag
     2581 acccgcaccc tttgtctcac gtacagttgc cgagccatct gaggaacgag actactcgga
     2641 tacctattgc cgaaatagga ttccaagccg ggcacatggc actcactaaa gacaagcccg
     2701 tgtccttgct cgtcaaacct ataaaccatt acgcgatcgt agccagtgca ttgttgaaac
     2761 aacagaacgg tatcatcaca caacgctcgc aaggaccccg ctgttcgtat cctttcaaga
     2821 gcgggcgcca gtgtgccact caaatcgata ctgggcccag cccgctctag ttcgatgatc
     2881 aacccccctt cggggggtcg atgcataagc ccgcaatact ccgtgctagg attaccgatg
     2941 cggcatctaa cagcaacagg catcccctcc gcagtaggat cgaggtgggg gaggatctta
     3001 atcaacagat caccgtctat ctccgccagc ggcactccca atacactacc aagattcagg
     3061 aactcagcgg cattagcaga cgcctgtatc accctgtggt catgttcgga cacaacgaga
     3121 agtgccccat gaggttgtat actccctgcc aggtgtatct cctcgtgctc gcagttagaa
     3181 tgacttgcag tgccgaacgc gggtgatcca gaggcgtgcc cagcaaccat ggtgaattcc
     3241 gcggaggctg gatcggtccc ggtgtcttct atggaggtca aaacagcgtg gatggcgtct
     3301 ccaggcgatc tgacggttca ctaaacgagc tctgcttata taggcctccc accgtacacg
     3361 cctaccctcg agaagcttga tatcgaattc cacggggttg gggttgcgcc ttttccaagg
     3421 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
     3481 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
     3541 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
     3601 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
     3661 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
     3721 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
     3781 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
     3841 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
     3901 ccagggggat cccccgggct gcaggaattc gggccgcggc cagctccggc gggcaggggg
     3961 ggcgctggag cgcagcgcag cgcagcccca tcagtccgca aagcggaccg agctggaagt
     4021 cgagcgctgc cgcgggaggc gggcgatggg ggcaggtgcc accggccgcg ccatggacgg
     4081 gccgcgcctg ctgctgttgc tgcttctggg ggtgtccctt ggaggtgcca aggaggcatg
     4141 ccccacaggc ctgtacacac acagcggtga gtgctgcaaa gcctgcaacc tgggcgaggg
     4201 tgtggcccag ccttgtggag ccaaccagac cgtgtgtgag ccctgcctgg acagcgtgac
     4261 gttctccgac gtggtgagcg cgaccgagcc gtgcaagccg tgcaccgagt gcgtggggct
     4321 ccagagcatg tcggcgccgt gcgtggaggc cgacgacgcc gtgtgccgct gcgcctacgg
     4381 ctactaccag gatgagacga ctgggcgctg cgaggcgtgc cgcgtgtgcg aggcgggctc
     4441 gggcctcgtg ttctcctgcc aggacaagca gaacaccgtg tgcgaggagt gccccgacgg
     4501 cacgtattcc gacgaggcca accacgtgga cccgtgcctg ccctgcaccg tgtgcgagga
     4561 caccgagcgc cagctccgcg agtgcacacg ctgggccgac gccgagtgcg aggagatccc
     4621 tggccgttgg attacacggt ccacaccccc agagggctcg gacagcacag cccccagcac
     4681 ccaggagcct gaggcacctc cagaacaaga cctcatagcc agcacggtgg caggtgtggt
     4741 gaccacagtg atgggcagct cccagcccgt ggtgacccga ggcaccaccg acaacctcat
     4801 ccctgtctat tgctccatcc tggctgctgt ggttgtgggc cttgtggcct acatagcctt
     4861 caagaggtgg aacaggggga tcctctagag tcgagtctag agtcgacaat caacctctgg
     4921 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4981 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5041 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5101 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5161 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5221 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5281 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     5341 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5401 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5461 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt
     5521 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5581 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5641 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5701 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5761 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     5821 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     5881 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5941 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6001 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     6061 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6121 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6181 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     6241 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6301 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6361 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     6421 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6481 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6541 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6601 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6661 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6721 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6781 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6841 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6901 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6961 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7021 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7081 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7141 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7201 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7261 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7321 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7381 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7441 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7501 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7561 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7621 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7681 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7741 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7801 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7861 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7921 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7981 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8041 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8101 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8161 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8221 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8281 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8341 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8401 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8461 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8521 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8581 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8641 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8701 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8761 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     8821 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     8881 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     8941 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     9001 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     9061 ttggccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc
     9121 aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     9181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     9241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     9301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     9361 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     9421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     9481 gcagtacatc tacgtattag tcatcgctat taccatg
//
