LOCUS       sequence-265016-        9271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid includes a TagBFP gene driven by a minimal CMV promoter.
            Cis-regulatory elements can be cloned into the single EcoRV site. 
            This plasmid can be used for generating a lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Roman Azcona MS, Fang Y, Carusillo A, Cathomen T, Mussolino C
  TITLE     A versatile reporter system for multiplexed screening of effective 
            epigenome editors.
  JOURNAL   Nat Protoc. 2020 Oct;15(10):3410-3440. doi: 
            10.1038/s41596-020-0380-y. Epub 2020 Sep 4.
  PUBMED    32887975
REFERENCE   2  (bases 1 to 9271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41596-020-0380-y"; journalName: "Nat Protoc"; date: 
            "2020-10"; volume: "15"; issue: "10"; pages: "3410-3440"
COMMENT     Sequence Label: pCCl-MCS_miniCMV_TagBFP
FEATURES             Location/Qualifiers
     source          1..9271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             219..399
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    446..571
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1064..1297
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1482..1526
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1793..1910
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    2047..2181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2283..2984)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        complement(3082..3120)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        3148..3658
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3577..3595
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    4662..5250
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4715..4735)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5133..5144)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5337..5570
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5642..5763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5803..5938
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5958..5977)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5959..5977)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5987..6004)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5987..6003)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5996..6018)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6145..6600
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6232..6251)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6442..6463
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6626..6730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6731..6799,6800..7591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6949..6968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7762..8350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8251..8270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8504..8521
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8638..8659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8674..8679,8680..8697,8698..8704)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8712..8728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8717..8739
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8736..8752
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8736..8752
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8771..8791
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8773..8791
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        8892..9271
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccggg gtctctctgg ttagaccaga
      241 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      301 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      361 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      421 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
      481 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
      541 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
      601 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
      661 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
      721 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
      781 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
      841 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
      901 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
      961 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1021 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1081 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1141 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1201 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1261 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1321 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1381 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1441 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1501 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1561 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1621 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1681 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     1741 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     1801 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     1861 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg
     1921 agactagcct cgatagatct gatcataatc agccatacca catttgtaga ggttttactt
     1981 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     2041 gttgttaact tgtttattgc agcttataat ggttacaaat aaggcaatag catcacaaat
     2101 ttcacaaata aggcattttt ttcactgcat tctagttttg gtttgtccaa actcatcaat
     2161 gtatcttatc atgtctggat ctcaaatccc tcggaagctg cgcctgtcat cgaattcctg
     2221 cagcccggtg catgactaag ctagctcagt tagcctcccc catctccctc gacgcggccg
     2281 ctttaattaa gcttgtgccc cagtttgcta gggaggtcgc agtatctggc cactgccacc
     2341 tcgtgctgct cgacgtaggt ctcgttgttg gcctccttga ttctttccag tctgtagtcc
     2401 acatagtaga cgccaggcat cttgaggttc ttagcgggtt tcttggatct atatgtggtc
     2461 ttgatgtttg cgatcagatg gctcccgccc acgagcttca gggccatgtc gtttctgcct
     2521 tccaggccgc cgtcagcggg gtacagcgtc tcggtgaagg cctcccagcc gagtgttttc
     2581 ttctgcatca cagggccgtt ggatgtgaag ttcacccctc tgatcttgac gttgtagatg
     2641 aggcagccgt cctggaggct ggtgtcctgg gtagcggtca gcacgccccc gtcttcgtat
     2701 gtggtgactc tctcccatgt gaagccctca gggaaggact gcttgaagaa gtcggggatg
     2761 ccctgggtgt ggttgatgaa ggtcttgctg ccgtagagga agctagtagc caggatgtcg
     2821 aaggcgaagg ggagagggcc gccctcgacc accttgattc tcatggtctg ggtgccctcg
     2881 tagggcttgc cttcgccctc ggatgtgcac ttgaagtgat ggttgtccac ggtgccctcc
     2941 atgtacagct tcatgtgcat gttctcctta atcagctcgc tcatggtgaa ttccgcggag
     3001 gctggatcgg tcccggtgtc ttctatggag gtcaaaacag cgtggatggc gtctccaggc
     3061 gatctgacgg ttcactaaac gagctctgct tatataggcc tcccaccgta cacgcctacc
     3121 ctcgagaagc ttgatatcga attccacggg gttggggttg cgccttttcc aaggcagccc
     3181 tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga
     3241 ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac
     3301 ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg
     3361 gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga
     3421 cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg
     3481 ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg
     3541 tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc
     3601 ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg
     3661 ggatcccccg ggctgcagga attcgggccg cggccagctc cggcgggcag ggggggcgct
     3721 ggagcgcagc gcagcgcagc cccatcagtc cgcaaagcgg accgagctgg aagtcgagcg
     3781 ctgccgcggg aggcgggcga tgggggcagg tgccaccggc cgcgccatgg acgggccgcg
     3841 cctgctgctg ttgctgcttc tgggggtgtc ccttggaggt gccaaggagg catgccccac
     3901 aggcctgtac acacacagcg gtgagtgctg caaagcctgc aacctgggcg agggtgtggc
     3961 ccagccttgt ggagccaacc agaccgtgtg tgagccctgc ctggacagcg tgacgttctc
     4021 cgacgtggtg agcgcgaccg agccgtgcaa gccgtgcacc gagtgcgtgg ggctccagag
     4081 catgtcggcg ccgtgcgtgg aggccgacga cgccgtgtgc cgctgcgcct acggctacta
     4141 ccaggatgag acgactgggc gctgcgaggc gtgccgcgtg tgcgaggcgg gctcgggcct
     4201 cgtgttctcc tgccaggaca agcagaacac cgtgtgcgag gagtgccccg acggcacgta
     4261 ttccgacgag gccaaccacg tggacccgtg cctgccctgc accgtgtgcg aggacaccga
     4321 gcgccagctc cgcgagtgca cacgctgggc cgacgccgag tgcgaggaga tccctggccg
     4381 ttggattaca cggtccacac ccccagaggg ctcggacagc acagccccca gcacccagga
     4441 gcctgaggca cctccagaac aagacctcat agccagcacg gtggcaggtg tggtgaccac
     4501 agtgatgggc agctcccagc ccgtggtgac ccgaggcacc accgacaacc tcatccctgt
     4561 ctattgctcc atcctggctg ctgtggttgt gggccttgtg gcctacatag ccttcaagag
     4621 gtggaacagg gggatcctct agagtcgagt ctagagtcga caatcaacct ctggattaca
     4681 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4741 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4801 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4861 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4921 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4981 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5041 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     5101 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5161 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5221 gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc
     5281 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5341 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     5401 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5461 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5521 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     5581 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     5641 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5701 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5761 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     5821 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5881 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5941 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     6001 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     6061 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6121 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6181 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6241 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6301 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6361 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6421 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6481 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6541 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     6601 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6661 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6721 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6781 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6841 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6901 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6961 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7021 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7081 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7141 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7201 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7261 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7321 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7381 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7441 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7501 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7561 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7621 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7681 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7741 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7801 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7861 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7921 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7981 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8041 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8101 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8161 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8221 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8281 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8341 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8401 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8461 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8521 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8581 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     8641 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     8701 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     8761 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttggcc
     8821 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
     8881 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     8941 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     9001 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     9061 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     9121 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     9181 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     9241 catctacgta ttagtcatcg ctattaccat g
//
