LOCUS       sequence-265018-        2892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2892)
  TITLE     Lashuel Lab plasmids
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-7 asyn K32R/K34R
FEATURES             Location/Qualifiers
     source          1..2892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     171..193
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     258..277
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        258..276
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             308..330
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(join(889..1680,1681..1749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1512..1531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1750..1854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1922..1940
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2069..2657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2558..2577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2811..2828
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taggaagatc ttccggaaga tcttcctatg gtgcactctc agtacaatct gctctgatgc
       61 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      121 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      181 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcccagcg attcgaactt
      241 ctgatagact tcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag
      301 aaataatttt gtttaacttt aagaaggaga tatacatatg gatgtattca tgaaaggact
      361 ttcaaaggcc aaggagggag ttgtggctgc tgctgagaaa accaaacagg gtgtggcaga
      421 agcagcagga aggacaagag agggtgttct ctatgtaggc tccaaaacca aggagggagt
      481 ggtgcatggt gtggcaacag tggctgagaa gaccaaagag caagtgacaa atgttggagg
      541 agcagtggtg acgggtgtga cagcagtagc ccagaagaca gtggagggag caggaagcat
      601 tgcagcagcc actggctttg tcaaaaagga ccagttgggc aagaatgaag aaggagcccc
      661 acaggaagga attctggaag atatgcctgt ggatcctgac aatgaggctt atgaaatgcc
      721 ttctgaggaa gggtatcaag actacgaacc tgaagcctaa gaaatatctt tgctcccagt
      781 ttcttgagat ctgctgacag atgttccatc caagcttatc gatgataagc tgtcaaacat
      841 gagaattaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      901 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      961 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1021 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1081 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1141 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1201 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1261 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1321 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1381 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1441 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1501 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1561 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     1621 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     1681 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1741 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1801 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1861 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     1921 aaataggcgt atcacgaggc cctttcgtct tcaagaataa aaggatctag gtgaagatcc
     1981 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2041 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2101 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2161 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     2221 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2281 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2341 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2401 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2461 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2521 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2581 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2641 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2701 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2761 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2821 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     2881 tttcacaccg ca
//
