LOCUS       sequence-265029-        9252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast reporter gene with glucocorticoid response element (GRE) 
            upstream of CYC1 promoter driving lacZ expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Schena M, Yamamoto KR
  TITLE     Mammalian glucocorticoid receptor derivatives enhance transcription 
            in yeast.
  JOURNAL   Science. 1988 Aug 19. 241(4868):965-7.
  PUBMED    3043665
REFERENCE   2  (bases 1 to 9252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1988 Aug 19. 241(4868):965-7."
COMMENT     Sequence Label: pUCdeltaSS-26X
FEATURES             Location/Qualifiers
     source          1..9252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3287..3303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3287..3303)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3300..3322)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3311..3327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3335..3341,3342..3359,3360..3365))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3380..3401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3518..3535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3689..4277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3769..3788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4448..5239,5240..5308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5071..5090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5309..5413)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5481..5499
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      5857..7199
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    6189..6236
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7791..8006
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             8007..8810
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
       61 ttggccgatt cattaatgca gctggcacga caggtttccc gacttaatcg ccttgcagca
      121 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      181 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
      241 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac
      301 tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc
      361 aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt
      421 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg
      481 tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct
      541 gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg
      601 cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc
      661 cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact
      721 cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag
      781 ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc
      841 ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc
      901 acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat
      961 cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat
     1021 gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg
     1081 ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag
     1141 cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt
     1201 tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg
     1261 gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat
     1321 ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat
     1381 cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac
     1441 gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc
     1501 ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac
     1561 cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag
     1621 acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc
     1681 gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac
     1741 tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc
     1801 ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc
     1861 gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc
     1921 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac
     1981 gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct
     2041 ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag
     2101 agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca
     2161 gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg
     2221 ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc
     2281 gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg
     2341 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg
     2401 gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc
     2461 tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca
     2521 cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta
     2581 tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat
     2641 gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg
     2701 caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc
     2761 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac
     2821 gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac
     2881 cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa
     2941 accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc
     3001 catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg
     3061 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg
     3121 ccatgtctgc ccgtatttcg cgtaaggaaa tccattgtac tgccggacca ccgactgtga
     3181 gccactccgg ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     3241 cggagttagg ggcctctagg ggtaccgagc tcgaattcgt aatcatgtca tagctgtttc
     3301 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3361 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     3421 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     3481 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     3541 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3601 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4741 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5041 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     5101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5401 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     5461 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattct
     5521 gaaccagtcc taaaacgagt aaataggacc ggcaattctt caagcaataa acaggaatac
     5581 caattattaa aagataactt agtcagatcg tacaataaag ctttgaagaa aaatgcgcct
     5641 tattcaatct ttgctataaa aaatggccca aaatctcaca ttggaagaca tttgatgacc
     5701 tcatttcttt caatgaaggg cctaacggag ttgactaatg ttgtgggaaa ttggagcgat
     5761 aagcgtgctt ctgccgtggc caggacaacg tatactcatc agataacagc aatacctgat
     5821 cactacttcg cactagtttc tcggtactat gcatatgatc caatatcaaa ggaaatgata
     5881 gcattgaagg atgagactaa tccaattgag gagtggcagc atatagaaca gctaaagggt
     5941 agtgctgaag gaagcatacg ataccccgca tggaatggga taatatcaca ggaggtacta
     6001 gactaccttt catcctacat aaatagacgc atataagtac gcatttaagc ataaacacgc
     6061 actatgccgt tcttctcatg tatatatata tacaggcaac acgcagatat aggtgcgacg
     6121 tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt cggaagcgct cgttttcgga
     6181 aacgctttga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcagag
     6241 cgcttttgaa aaccaaaagc gctctgaaga cgcactttca aaaaaccaaa aacgcaccgg
     6301 actgtaacga gctactaaaa tattgcgaat accgcttcca caaacattgc tcaaaagtat
     6361 ctctttgcta tatatctctg tgctatatcc ctatataacc tacccatcca cctttcgctc
     6421 cttgaacttg catctaaact cgacctctac attttttatg tttatctcta gtattactct
     6481 ttagacaaaa aaattgtagt aagaactatt catagagtga atcgaaaaca atacgaaaat
     6541 gtaaacattt cctatacgta gtatatagag acaaaataga agaaaccgtt cataattttc
     6601 tgaccaatga agaatcatca acgctatcac tttctgttca caaagtatgc gcaatccaca
     6661 tcggtataga atataatcgg ggatgccttt atcttgaaaa aatgcacccg cagcttcgct
     6721 agtaatcagt aaacgcggga agtggagtca ggcttttttt atggaagaga aaatagacac
     6781 caaagtagcc ttcttctaac cttaacggac ctacagtgca aaaagttatc aagagactgc
     6841 attatagagc gcacaaagga gaaaaaaagt aatctaagat gctttgttag aaaaatagcg
     6901 ctctcgggat gcatttttgt agaacaaaaa agaagtatag attctttgtt ggtaaaatag
     6961 cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg tttgaaaaat
     7021 tagcgctctc gcgttgcatt tttgttttac aaaaatgaag cacagattct tcgttggtaa
     7081 aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga
     7141 aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat gaagcacaga tgcttcgtta
     7201 acaaagatat gctattgaag tgcaagatgg aaacgcagaa aatgaaccgg ggatgcgacg
     7261 tgcaagatta cctatgcaat agatgcaata gtttctccag gaaccgaaat acatacattg
     7321 tcttccgtaa agcgctagac tatatattat tatacaggtt caaatatact atctgtttca
     7381 gggaaaactc ccaggttcgg atgttcaaaa ttcaatgatg ggtaacaagt acgatcgtaa
     7441 atctgtaaaa cagtttgtcg gatattaggc tgtatctcct caaagcgtat tcgaatatca
     7501 ttgagaagct gcagcgtcac atcggataat aatgatggca gccattgtag aagtgccttt
     7561 tgcatttcta gtctctttct cggtctagct agttttacta catcgcgaag atagaatctt
     7621 agatcacact gcctttgctg agctggatca atagagtaac aaaagagtgg taaggcctcg
     7681 ttaaaggaca aggacctgag cggaagtgta tcgtacagta gacggagtat actagtatag
     7741 tctatagtcc gtggaattct catgtttgac agcttatcat cgataagctt ttcaattcat
     7801 catttttttt ttattctttt ttttgatttc ggtttccttg aaattttttt gattcggtaa
     7861 tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca
     7921 tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa
     7981 aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta
     8041 ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact
     8101 tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag
     8161 gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg
     8221 gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaagaca
     8281 gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa
     8341 tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg
     8401 gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg atgttagcag
     8461 aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact gttgacattg
     8521 cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag
     8581 atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac aagggagacg
     8641 cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct gacattatta
     8701 ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca
     8761 gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat
     8821 tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt
     8881 tattaccctc gacctcgact gtacaggatg ttctagctac tcgagtagct agaacatcct
     8941 gtacagtcga gtagctagaa catcctgtac agtcgagcag atccgccagg cgtgtatata
     9001 gcgtggatgg ccaggcaact ttagtgctga cacatacagg catatatata tgtgtgcgac
     9061 gacacatgat catatggcat gcatgtgctc tgtatgtata taaaactctt gttttcttct
     9121 tttctctaaa tattctttcc ttatacatta ggtcctttgt agcataaatt actatacttc
     9181 tatagacacg caaacacaaa tacacacact aaattaataa tgaccggatc cggagcttgg
     9241 ctgttgcccg tc
//
