LOCUS       sequence-265031-        9917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression vector for fusion of Gal4 AA 1-848 to hormone 
            binding domain of estrogen receptor a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9917)
  TITLE     Picard lab plasmids
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHCA/GAL4(848).ER
FEATURES             Location/Qualifiers
     source          1..9917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    93..114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(129..134,135..152,153..159)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    167..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     172..194
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     191..207
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     191..207
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        228..246
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     274..290
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     275..291
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1077..1781
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1809..2249
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             4359..5294
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4407..5300
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     primer_bind     complement(5314..5333)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5315..5333)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5340..5357)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5340..5356)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5349..5371)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5497..5952
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5584..5603)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5794..5815
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6212..6874)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(6875..7062)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(7209..7228)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7328..7350
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7388..7406)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7454..7957
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7987..8091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8092..8160,8161..8952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8310..8329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9123..9711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9612..9631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9876..9893
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
       61 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      121 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      181 caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac
      241 taaagggaac aaaagctggt accgggcccc ccctcgaggt cgacggtatc gataagcttg
      301 atatcgaatt cctgcagccc gggggatccg ggatcgaaga aatgatggta aatgaaatag
      361 gaaatcaagg agcatgaagg caaaagacaa atataagggt cgaacgaaaa ataaagtgaa
      421 aagtgttgat atgatgtatt tggctttgcg gcgccgaaaa aacgagttta cgcaattgca
      481 caatcatgct gactctgtgg cggacccgcg ctcttgccgg cccggcgata acgctgggcg
      541 tgaggctgtg cccggcggag ttttttgcgc ctgcattttc caaggtttac cctgcgctaa
      601 ggggcgagat tggagaagca ataagaatgc cggttggggt tgcgatgatg acgaccacga
      661 caactggtgt cattatttaa gttgccgaaa gaacctgagt gcatttgcaa catgagtata
      721 ctagaagaat gagccaagac ttgcgagacg cgagtttgcc ggtggtgcga acaatagagc
      781 gaccatgacc ttgaaggtga gacgcgcata accgctagag tactttgaag aggaaacagc
      841 aatagggttg ctaccagtat aaatagacag gtacatacaa cactggaaat ggttgtctgt
      901 ttgagtacgc tttcaattca tttgggtgtg cactttatta tgttacaata tggaagggaa
      961 ctttacactt ctcctatgca catatattaa ttaaagtcca atgctagtag agaagggggg
     1021 taacacccct ccgcgctctt ttccgatttt tttctaaacc gtggaatatt tcggatatcc
     1081 ttttgttgtt tccgggtgta caatatggac ttcctctttt ctggcaacca aacccataca
     1141 tcgggattcc tataatacct tcgttggtct ccctaacatg taggtggcgg aggggagata
     1201 tacaatagaa cagataccag acaagacata atgggctaaa caagactaca ccaattacac
     1261 tgcctcattg atggtggtac ataacgaact aatactgtag ccctagactt gatagccatc
     1321 atcatatcga agtttcacta ccctttttcc atttgccatc tattgaagta ataataggcg
     1381 catgcaactt cttttctttt tttttctttt ctctctcccc cgttgttgtc tcaccatatc
     1441 cgcaatgaca aaaaaatgat ggaagacact aaaggaaaaa attaacgaca aagacagcac
     1501 caacagatgt cgttgttcca gagctgatga ggggtatctc gaagcacacg aaactttttc
     1561 cttccttcat tcacgcacac tactctctaa tgagcaacgg tatacggcct tccttccagt
     1621 tacttgaatt tgaaataaaa aaaagtttgc tgtcttgcta tcaagtataa atagacctgc
     1681 aattattaat cttttgtttc ctcgtcattg ttctcgttcc ctttcttcct tgtttctttt
     1741 tctgcacaat atttcaagct ataccaagca tacaatcaac tccaagcttg aagcaagcct
     1801 cctgaaagat gaagctactg tcttctatcg aacaagcatg cgatatttgc cgacttaaaa
     1861 agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac aactgggagt
     1921 gtcgctactc tcccaaaacc aaaaggtctc cgctgactag ggcacatctg acagaagtgg
     1981 aatcaaggct agaaagactg gaacagctat ttctactgat ttttcctcga gaagaccttg
     2041 acatgatttt gaaaatggat tctttacagg atataaaagc attgttaaca ggattatttg
     2101 tacaagataa tgtgaataaa gatgccgtca cagatagatt ggcttcagtg gagactgata
     2161 tgcctctaac attgagacag catagaataa gtgcgacatc atcatcggaa gagagtagta
     2221 acaaaggtca aagacagttg actgtatcga ttgactcggc agctcatcat gataactcca
     2281 caattccgtt ggattttatg cccagggatg ctcttcatgg atttgattgg tctgaagagg
     2341 atgacatgtc ggatggcttg cccttcctga aaacggaccc caacaataat gggttctttg
     2401 gcgacggttc tctcttatgt attcttcgat ctattggctt taaaccggaa aattacacga
     2461 actctaacgt taacaggctc ccgaccatga ttacggatag atacacgttg gcttctagat
     2521 ccacaacatc ccgtttactt caaagttatc tcaataattt tcacccctac tgccctatcg
     2581 tgcactcacc gacgctaatg atgttgtata ataaccagat tgaaatcgcg tcgaaggatc
     2641 aatggcaaat cctttttaac tgcatattag ccattggagc ctggtgtata gagggggaat
     2701 ctactgatat agatgttttt tactatcaaa atgctaaatc tcatttgacg agcaaggtct
     2761 tcgagtcagg ttccataatt ttggtgacag ccctacatct tctgtcgcga tatacacagt
     2821 ggaggcagaa aacaaatact agctataatt ttcacagctt ttccataaga atggccatat
     2881 cattgggctt gaatagggac ctcccctcgt ccttcagtga tagcagcatt ctggaacaaa
     2941 gacgccgaat ttggtggtct gtctactctt gggagatcca attgtccctg ctttatggtc
     3001 gatccatcca gctttctcag aatacaatct ccttcccttc ttctgtcgac gatgtgcagc
     3061 gtaccacaac aggtcccacc atatatcatg gcatcattga aacagcaagg ctcttacaag
     3121 ttttcacaaa aatctatgaa ctagacaaaa cagtaactgc agaaaaaagt cctatatgtg
     3181 caaaaaaatg cttgatgatt tgtaatgaga ttgaggaggt ttcgagacag gcaccaaagt
     3241 ttttacaaat ggatatttcc accaccgctc taaccaattt gttgaaggaa cacccttggc
     3301 tatcctttac aagattcgaa ctgaagtgga aacagttgtc tcttatcatt tatgtattaa
     3361 gagatttttt cactaatttt acccagaaaa agtcacaact agaacaggat caaaatgatc
     3421 atcaaagtta tgaagttaaa cgatgctcca tcatgttaag cgatgcagca caaagaactg
     3481 ttatgtctgt aagtagctat atggacaatc ataatgtcac cccatatttt gcctggaatt
     3541 gttcttatta cttgttcaat gcagtcctag tacccataaa gactctactc tcaaactcaa
     3601 aatcgaatgc tgagaataac gagaccgcac aattattaca acaaattaac actgttctga
     3661 tgctattaaa aaaactggcc acttttaaaa tccagacttg tgaaaaatac attcaagtac
     3721 tggaagaggt atgtgcgccg tttctgttat cacagtgtgc aatcccatta ccgcatatca
     3781 gttataacaa tagtaatggt agcgccatta aaaatattgt cggttctgca actatcgccc
     3841 aataccctac tcttccggag gaaaatgtca acaatatcag tgttaaatat gtttctcctg
     3901 gctcagtagg gccttcacct gtgccattga aatcaggagc aagtttcagt gatctagtca
     3961 agctgttatc taaccgtcca ccctctcgta actctccagt gacaatacca agaagcacac
     4021 cttcgcatcg ctcagtcacg ccttttctag ggcaacagca acagctgcaa tcattagtgc
     4081 cactgacccc gtctgctttg tttggtggcg ccaattttaa tcaaagtggg aatattgctg
     4141 atagctcatt gtccttcact ttcactaaca gtagcaacgg tccgaacctc ataacaactc
     4201 aaacaaattc tcaagcgctt tcacaaccaa ttgcctcctc taacgttcat gataacttca
     4261 tgaataatga aatcacggct agtaaaattg atgatggtaa taattcaaaa ccactgtcac
     4321 ctggttggac ggaccaaact gcgtataacg cgagatctgc tggagacatg agagctgcca
     4381 acctttggcc aagcccgctc atgatcaaac gctctaagaa gaacagcctg gccttgtccc
     4441 tgacggccga ccagatggtc agtgccttgt tggatgctga gccccccata ctctattccg
     4501 agtatgatcc taccagaccc ttcagtgaag cttcgatgat gggcttactg accaacctgg
     4561 cagacaggga gctggttcac atgatcaact gggcgaagag ggtgccaggc tttgtggatt
     4621 tgaccctcca tgatcaggtc caccttctag aatgtgcctg gctagagatc ctgatgattg
     4681 gtctcgtctg gcgctccatg gagcacccag tgaagctact gtttgctcct aacttgctct
     4741 tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac atgctgctgg
     4801 ctacatcatc tcggttccgc atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat
     4861 ctattatttt gcttaattct ggagtgtaca catttctgtc cagcaccctg aagtctctgg
     4921 aagagaagga ccatatccac cgagtcctgg acaagatcac agacactttg atccacctga
     4981 tggccaaggc aggcctgacc ctgcagcagc agcaccagcg gctggcccag ctcctcctca
     5041 tcctctccca catcaggcac atgagtaaca aaggcatgga gcatctgtac agcatgaagt
     5101 gcaagaacgt ggtgcccctc tatgacctgc tgctggagat gctggacgcc caccgcctac
     5161 atgcgcccac tagccgtgga ggggcatccg tggaggagac ggaccaaagc cacttggcca
     5221 ctgcgggctc tacttcatcg cattccttgc aaaagtatta catcacgggg gaggcagagg
     5281 gtttccctgc cacagtctga gagctccaat tcgccctata gtgagtcgta ttacaattca
     5341 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     5401 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     5461 ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa
     5521 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5581 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5641 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5701 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5761 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5821 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5881 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5941 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     6001 catagatccg tcgagttcaa gagaaaaaaa aagaaaaagc aaaaagaaaa aaggaaagcg
     6061 cgcctcgttc agaatgacac gtatagaatg atgcattacc ttgtcatctt cagtatcata
     6121 ctgttcgtat acatacttac tgacattcat aggtatacat atatacacat gtatatatat
     6181 cgtatgctgc agctttaaat aatcggtgtc actacataag aacacctttg gtggagggaa
     6241 catcgttggt accattgggc gaggtggctt ctcttatggc aaccgcaaga gccttgaacg
     6301 cactctcact acggtgatga tcattcttgc ctcgcagaca atcaacgtgg agggtaattc
     6361 tgctagcctc tgcaaagctt tcaagaaaat gcgggatcat ctcgcaagag agatctccta
     6421 ctttctccct ttgcaaacca agttcgacaa ctgcgtacgg cctgttcgaa agatctacca
     6481 ccgctctgga aagtgcctca tccaaaggcg caaatcctga tccaaacctt tttactccac
     6541 gcacggcccc tagggcctct ttaaaagctt gaccgagagc aatcccgcag tcttcagtgg
     6601 tgtgatggtc gtctatgtgt aagtcaccaa tgcactcaac gattagcgac cagccggaat
     6661 gcttggccag agcatgtatc atatggtcca gaaaccctat acctgtgtgg acgttaatca
     6721 cttgcgattg tgtggcctgt tctgctactg cttctgcctc tttttctggg aagatcgagt
     6781 gctctatcgc taggggacca ccctttaaag agatcgcaat ctgaatcttg gtttcatttg
     6841 taatacgctt tactagggct ttctgctctg tcatctttgc cttcgtttat cttgcctgct
     6901 cattttttag tatattcttc gaagaaatca cattacttta tataatgtat aattcattat
     6961 gtgataatgc caatcgctaa gaaaaaaaaa gagtcatccg ctaggtggaa aaaaaaaaat
     7021 gaaaatcatt accgaggcat aaaaaaatat agagtgtact agaggaggcc aagagtaata
     7081 gaaaaagaaa attgcgggaa aggactgtgt tatgacttcc ctgactaatg ccgtgttcaa
     7141 acgatacctg gcagtgactc ctagcgctca ccaagctctt aaaacgggaa tttatggtgc
     7201 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     7261 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     7321 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     7381 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     7441 tagggtcctt ttcatcacgt gctataaaaa taattataat ttaaattttt taatataaat
     7501 atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc
     7561 gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttatc ttgctcttcc
     7621 tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaagac cacacacgaa
     7681 aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt taatctgctt
     7741 ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt taaacctttg
     7801 tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt tctaaaatcc
     7861 aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt ccatcattaa
     7921 aagatacgag gcgcgtgtaa gttacaggca agcgatccgt caggtggcac ttttcgggga
     7981 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8041 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8101 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8161 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8221 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8281 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8341 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8401 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8461 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8521 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8581 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8641 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8701 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8761 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8821 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8881 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8941 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9001 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9061 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9121 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9181 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9241 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9301 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9361 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9421 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9481 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9541 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9601 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9661 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9721 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctggcct tttgctcaca
     9781 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9841 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9901 aagagcgccc aatacgc
//
