LOCUS       sequence-26505-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Hackett EA, Esch RK, Maleri S, Errede B
  TITLE     A family of destabilized cyan fluorescent proteins as 
            transcriptional reporters in S. cerevisiae.
  JOURNAL   Yeast. 2006 Apr 15. 23(5):333-49.
  PUBMED    16598699
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Apr 15. 23(5):333-49."
COMMENT     Sequence Label: pNC843
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..275)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(684..707)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gggacaactc cagtgaaaag ttcttctcct ttactggatc cgtgcatacc acctcttagc
       61 cttagcacaa gatgtaaggt ggactccttc tgaatgttgt aatcagacag cgttctaccg
      121 tcttctagct gcttaccggc aaagatcaat ctttgttgat ctggagggat accttccttg
      181 tcttgaattt tcgacttaac gttgtcgatg gtatcggaag attcaacttc caatgttatg
      241 gttttaccgg tcaaagtctt gacgaaaatc tgcataatct attagttaaa gtaaagtggg
      301 agggagagtt cgagagttct tttcgtattg tagtagatag aagatatact tatccttatc
      361 cgtacagttc ttgctctttc tgaagtagca ctttcggaag aagaaagata agcaggcgaa
      421 ttgatccggt aatttagtgt gtgtatttgt gtttgcgtgt ctatagaagt atagtaattt
      481 atgctacaaa ggacctaatg tataaggaaa gaatatttag agaaaagaag aaaacaagag
      541 ttttatatac atacagagca catgcatgcc atatgatcat gtgtcgtcgc acacatatat
      601 atatgcctgt atgtgtcagc actaaagttg cctggccatc cacgctatat acacgcctgg
      661 cagatccggg gttttttctc cttgacgtta aagtatagag gtatattaac aattttttgt
      721 tgatactttt attacatttg aataagaagt aatacaaacc gaaaatgttg aaagtattag
      781 ttaaagtggt tatgcagttt ttgcatttat atatctgtta atagatcaaa aatcatcgct
      841 tcgctgatta atnnccnaaa annnggctaa aaactaatcg catnancatc cnatggt
//
