LOCUS       sequence-265050-        3272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate Level 0 S module for N-terminal fluorescent protein 
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Luginbuehl LH, El-Sharnouby S, Wang N, Hibberd JM
  TITLE     Fluorescent reporters for functional analysis in rice leaves.
  JOURNAL   Plant Direct. 2020 Feb 11;4(2):e00188. doi: 10.1002/pld3.188. 
            eCollection 2020 Feb.
  PUBMED    32072132
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-02-11"; volume: "4(2):e00188. doi"; pages: " 
            10.1002/pld3.188. eCollection 2020 Feb"
COMMENT     Sequence Label: pL0M-S-mNeonGreen-EC18153
FEATURES             Location/Qualifiers
     source          1..3272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1262..1279)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1372..1960)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1452..1471)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(3145..3249)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggccacgtg tcttgtccag agctccactc tgtggtctca aatggcgtcg aagggcgaag
      421 aggataatat ggcctcgctc ccggcgactc acgaattgca tatattcggg tcgattaacg
      481 gggtcgactt cgatatggtg ggccaaggta cagggaatcc gaacgatgga tatgaagaac
      541 tcaacctgaa gtcgactaag ggagatctcc agttttcgcc ttggatactg gtcccgcata
      601 taggctacgg attccatcag tatttgccgt acccagacgg aatgtccccg tttcaggcag
      661 ccatggttga tggatctgga taccaagtgc atcgcactat gcagtttgag gatggtgcca
      721 gtcttacggt taattatcgg tacacatacg aagggtccca cattaagggc gaggcccaag
      781 ttaaaggtac gggattccca gccgacgggc ccgtgatgac caattcgctt actgcagctg
      841 attggtgtcg gagcaaaaag acgtacccca atgataaaac tatcatctca actttcaaat
      901 ggtcatatac cacaggaaac ggtaagagat ataggtctac ggccaggact acatatacgt
      961 tcgcaaaacc tatggcagcg aactacttga agaatcaacc aatgtacgtc tttcgcaaaa
     1021 cggagctgaa acactctaag acagaattga acttcaaaga atggcaaaaa gcgttcacgg
     1081 acgtgatggg aatggatgaa ctctataaag gaggttgaga ccacgaagtg ggtacctgga
     1141 gcacaagact ggcctcatgg gccttcactt cactgcccgc tttccagtcg ggaaacctgt
     1201 cgtgccagct gcattaacat ggtcatagct gtttccttgc gtattgggcg ctctccgctt
     1261 cctcgctcac tgactcgctg cgctcggtcg ttcgggtaaa gcctggggtg cctaatgagc
     1321 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1441 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1501 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1561 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1621 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1681 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1741 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1801 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1861 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1921 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1981 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2041 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2101 aagtatatat gagtaaactt ggtctgacag ttattattta cccaccactt tggtaatttc
     2161 gcctttcacg taatgcacaa attcttccag ctgatccgca cggctggcca gacgatcttc
     2221 ttcctggccc agatacgcct gacgcgcttc cagaatcacc ggctgatact gcgccggcag
     2281 acgttccatg gcccaatccg ccgccacatc tttcggcgca attttgccgg tcaccgcgct
     2341 ataccaaata cggctcaggg tcagcaccac gttgcgttca tcacccgccc aatccggcgg
     2401 gctgttccac agggtcaggg tttcgttcag cgcttcaaac aggtcctgtt ccggcaccgg
     2461 atcaaacagt tcttccgctg ccggaccaac cagcgccacg ctatgttcac gcgctttggt
     2521 cagcagaatg gccagatcga tatcaatggt cgccggttca aaaatgccgg ccagaatatc
     2581 gttacgctgc cattcgccaa actgcagctc acgtttcgcc ggataacgcc acggaataat
     2641 gtcatcatgc accacgatgg tcacttccac cgcacgcaga atttcgcttt cacccgggct
     2701 cgcgctggtt tccagcagat cgttaatcag cgcacgacgg gtggtttcat ccagacgcac
     2761 ggtcacggtc accagcagat caatatcgct atgcggtttc aggccgccat ccaccgcgct
     2821 gccatacaga tgcacggcca gcagggtcgg ttccagatga cgttcaatca cgcccaccac
     2881 ttcagacagc tgggtgctca cttccgcaat caccgcttca cgcataatgt tcagtttggt
     2941 tttcggacgg ctgccctgct gggtcacatc gttgctgctc cacagcatca ggcaacggcc
     3001 caccgcataa cgcgcctgct gtttgctcgc acgcggcatg ctctgcacgc caaaaaagca
     3061 atcatagcac gccataaaca ccgccaccgc accattaccg ccgctacgtt cggtcagggt
     3121 acggctccaa ttacggctac gcatactctt cctttttcaa tattattgaa gcatttatca
     3181 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3241 ggttccgcgc acatttcccc gaaaagtgcc ac
//
