LOCUS       sequence-26506-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Hackett EA, Esch RK, Maleri S, Errede B
  TITLE     A family of destabilized cyan fluorescent proteins as 
            transcriptional reporters in S. cerevisiae.
  JOURNAL   Yeast. 2006 Apr 15. 23(5):333-49.
  PUBMED    16598699
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Apr 15. 23(5):333-49."
COMMENT     Sequence Label: pNC844
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..274)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     primer_bind     complement(683..706)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gganaactcc ngtgaaaagt tcttctcctt tactggatcc gtgnnnnnnn cctcttagcc
       61 ttagcacaag atgtaaggtg gactccttct gaatgttgta atcagacagc gttctaccgt
      121 cttctagctg cttaccggca aagatcaatc tttgttgatc tggagggata ccttccttgt
      181 cttgaatttt cgacttaacg ttgtcgatgg tatcggaaga ttcaacttcc aatgttatgg
      241 ttttaccggt caaagtcttg acgaaaatct gcataatcta ttagttaaag taaagtggga
      301 gggagagttc gagagttctt ttcgtattgt agtagataga agatatactt atccttatcc
      361 gtacagttct tgctctttct gaagtagcac tttcggaaga agaaagataa gcaggcgaat
      421 tgatccggta atttagtgtg tgtatttgtg tttgcgtgtc tatagaagta tagtaattta
      481 tgctacaaag gacctaatgt ataaggaaag aatatttaga gaaaagaaga aaacaagagt
      541 tttatataca tacagagcac atgcatgcca tatgatcatg tgtcgtcgca cacatatata
      601 tatgcctgta tgtgtcagca ctaaagttgc ctggccatcc acgctatata cacgcctggc
      661 agatccgggg ttttttctcc ttgacgttaa agtatagagg tatattaaca antttttgtt
      721 gatactttta ttacatttga ataagaagta atacaaaccg aaaatgntga aagtattagt
      781 taaagtggtt atgcagtttt tgcatttata tatctgntaa tagatcaaaa atcatcgctt
      841 cgctgantaa ttaccccaga aatnaggnta aaaaactaat cgcattatca tcctatggt
//
