LOCUS       sequence-265063-        3281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate Level 0 S module for N-terminal fluorescent protein 
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Luginbuehl LH, El-Sharnouby S, Wang N, Hibberd JM
  TITLE     Fluorescent reporters for functional analysis in rice leaves.
  JOURNAL   Plant Direct. 2020 Feb 11;4(2):e00188. doi: 10.1002/pld3.188. 
            eCollection 2020 Feb.
  PUBMED    32072132
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-02-11"; volume: "4(2):e00188. doi"; pages: " 
            10.1002/pld3.188. eCollection 2020 Feb"
COMMENT     Sequence Label: pL0M-S-mClover3-EC18157
FEATURES             Location/Qualifiers
     source          1..3281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1271..1288)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1381..1969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1461..1480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(3154..3258)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggccacgtg tcttgtccag agctccactc tgtggtctca aatggcgtcc aagggggaag
      421 aactgtttac cggagtcgtt cctattctgg tcgaactgga tggtgatgtc aatggccaca
      481 agttctcggt ccggggtgaa ggtgagggtg acgcaacaaa cggcaagctg acactcaagt
      541 ttatttgcac aacaggcaag ctgcctgtcc cgtggccaac acttgttacg acattcggtt
      601 atggtgtcgc atgtttttca cgctatcctg accacatgaa gcagcatgat ttcttcaaat
      661 cggccatgcc cgaaggttat gttcaggagc ggactatatc tttcaaggac gacggaacgt
      721 acaaaacgcg cgccgaagtt aagttcgaag gtgacacctt ggtgaatcgg attgagttga
      781 agggaatcga ctttaaggag gacgggaaca tacttggaca caagttggag tacaatttta
      841 attctcatta tgtctacata accgcggaca agcaaaagaa ttgtataaag gcgaatttta
      901 aaataaggca taatgtggaa gatggaagcg tgcagcttgc tgatcattat caacaaaata
      961 cgccgatagg agatgggcca gtgttgctgc ctgacaacca ctacctctcc catcagagca
     1021 aattgtcaaa agacccgaat gaaaaacgcg atcacatggt cctcctggag tttgtcaccg
     1081 cagctggtat cacgcacggc atggatgaac tttacaaagg aggttgagac cacgaagtgg
     1141 gtacctggag cacaagactg gcctcatggg ccttcacttc actgcccgct ttccagtcgg
     1201 gaaacctgtc gtgccagctg cattaacatg gtcatagctg tttccttgcg tattgggcgc
     1261 tctccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc
     1321 ctaatgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1381 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1441 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1501 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1561 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1621 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1681 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1741 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1801 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1861 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1921 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1981 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2041 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2101 atcaatctaa agtatatatg agtaaacttg gtctgacagt tattatttac ccaccacttt
     2161 ggtaatttcg cctttcacgt aatgcacaaa ttcttccagc tgatccgcac ggctggccag
     2221 acgatcttct tcctggccca gatacgcctg acgcgcttcc agaatcaccg gctgatactg
     2281 cgccggcaga cgttccatgg cccaatccgc cgccacatct ttcggcgcaa ttttgccggt
     2341 caccgcgcta taccaaatac ggctcagggt cagcaccacg ttgcgttcat cacccgccca
     2401 atccggcggg ctgttccaca gggtcagggt ttcgttcagc gcttcaaaca ggtcctgttc
     2461 cggcaccgga tcaaacagtt cttccgctgc cggaccaacc agcgccacgc tatgttcacg
     2521 cgctttggtc agcagaatgg ccagatcgat atcaatggtc gccggttcaa aaatgccggc
     2581 cagaatatcg ttacgctgcc attcgccaaa ctgcagctca cgtttcgccg gataacgcca
     2641 cggaataatg tcatcatgca ccacgatggt cacttccacc gcacgcagaa tttcgctttc
     2701 acccgggctc gcgctggttt ccagcagatc gttaatcagc gcacgacggg tggtttcatc
     2761 cagacgcacg gtcacggtca ccagcagatc aatatcgcta tgcggtttca ggccgccatc
     2821 caccgcgctg ccatacagat gcacggccag cagggtcggt tccagatgac gttcaatcac
     2881 gcccaccact tcagacagct gggtgctcac ttccgcaatc accgcttcac gcataatgtt
     2941 cagtttggtt ttcggacggc tgccctgctg ggtcacatcg ttgctgctcc acagcatcag
     3001 gcaacggccc accgcataac gcgcctgctg tttgctcgca cgcggcatgc tctgcacgcc
     3061 aaaaaagcaa tcatagcacg ccataaacac cgccaccgca ccattaccgc cgctacgttc
     3121 ggtcagggta cggctccaat tacggctacg catactcttc ctttttcaat attattgaag
     3181 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3241 acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
