LOCUS       sequence-265066-        5791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Myogenin core promoter in a luciferase reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Sun J, Kamei CN, Layne MD, Jain MK, Liao JK, Lee ME, Chin MT
  TITLE     Regulation of myogenic terminal differentiation by the hairy-related
            transcription factor CHF2.
  JOURNAL   J Biol Chem. 2001 May 25;276(21):18591-6. doi: 
            10.1074/jbc.M101163200. Epub 2001 Feb 22.
  PUBMED    11279181
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M101163200"; journalName: "J Biol Chem"; date: 
            "2001-05-25- 25"; volume: "276"; issue: "21"; pages: "18591-6"
COMMENT     Sequence Label: pGL2-Basic-Myogenin core promoter
FEATURES             Location/Qualifiers
     source          1..5791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             260..1912
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(326..344)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2153..2218
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2348..2368
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2793..2927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3069..3086)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3240..3828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3320..3339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3999..4790,4791..4859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4622..4641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4860..4964)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4991..5446
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5078..5097)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5288..5309
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5636..5757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 taccgagctc ttacgcgtgc tagctcgagc ctgcagggtg gggtgggggc aaaaggagag
       61 ggaaggggaa tcacatgtaa tccactggaa acgtcttgat gtgcagcaac agcttagagg
      121 ggggctcagg tttctgtggc gttggctata tttatctctg ggttcatgcc agcagggagg
      181 gtttaaatgg cacccagcag ttggcgtgag gggctgcggg agcttggggg aagcttggca
      241 ttccggtact gttggtaaaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt
      301 ctatcctcta gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
      361 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc
      421 ggaatacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
      481 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
      541 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
      601 gctcaacagt atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca
      661 aaaaattttg aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc
      721 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
      781 cggttttaat gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact
      841 gataatgaat tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac
      901 tgcctgcgtc agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
      961 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
     1021 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt
     1081 acgatccctt caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt
     1141 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
     1201 tgggggcgca cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc
     1261 agggatacga caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     1321 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     1381 ggatctggat accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg
     1441 acctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     1501 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     1561 agttgaccgc ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt
     1621 ggaatcgata ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga
     1681 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     1741 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     1801 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     1861 aatcagagag atcctcataa aggccaagaa gggcggaaag tccaaattgt aaaatgtaac
     1921 tgtattcagc gatgacgaaa ttcttagcta ttgtaatact gcgatgagtg gcagggcggg
     1981 gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggttgcta cgcctgaata
     2041 agtgataata agcggatgaa tggcagaaat tcgccggatc tttgtgaagg aaccttactt
     2101 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     2161 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     2221 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     2281 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     2341 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     2401 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     2461 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
     2521 tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
     2581 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
     2641 taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
     2701 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2761 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2821 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2881 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgt cgaccgatgc
     2941 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     3001 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     3061 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3121 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3241 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3661 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3901 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3961 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4021 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4081 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4141 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4201 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4261 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4321 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4381 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4441 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4501 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4561 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4621 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4681 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4741 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4801 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4861 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4921 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4981 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5041 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5101 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5161 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5221 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5281 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5341 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5401 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc
     5461 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc
     5521 aagctaccat gataagtaag taatattaag gtacgtggag gttttacttg ctttaaaaaa
     5581 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     5641 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5701 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatgg
     5761 tactgtaact gagctaacat aacccgggag g
//
