LOCUS       sequence-265068-        3218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate Level 0 S module for N-terminal fluorescent protein 
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Luginbuehl LH, El-Sharnouby S, Wang N, Hibberd JM
  TITLE     Fluorescent reporters for functional analysis in rice leaves.
  JOURNAL   Plant Direct. 2020 Feb 11;4(2):e00188. doi: 10.1002/pld3.188. 
            eCollection 2020 Feb.
  PUBMED    32072132
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-02-11"; volume: "4(2):e00188. doi"; pages: " 
            10.1002/pld3.188. eCollection 2020 Feb"
COMMENT     Sequence Label: pL0M-S-mKO-kappa-EC18154
FEATURES             Location/Qualifiers
     source          1..3218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1208..1225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1318..1906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1398..1417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(3091..3195)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggccacgtg tcttgtccag agctccactc tgtggtctca aatggcgtct gttatcaagc
      421 ccgagatgaa aatgcggtac tacatggatg gctctgtcaa tggtcacgaa tttactatcg
      481 agggagaagg tacagggcgg ccatacgaag ggcatcaaga gatgactctt cgcgtgacta
      541 tggccgaagg cggacctatg ccatttgcat tcgatttggt ttcgcacgtc ttttgctatg
      601 gacatcgcgt tttcacgaaa taccctgaag aaattcctga ttacttcaag caagcctttc
      661 ccgaagggct gagctgggaa agatcattgg agttcgaaga tgggggtagt gcaagtgtga
      721 gtgcccatat aagcttgcgc ggcaatacat tctatcacaa gtcgaagttc actggagtca
      781 attttcctgc tgatggacct atcatgcaga accagtcagt ggactgggag ccaagtacag
      841 aaaaaatcac agcatcagat ggagttctca aaggcgacgt gaccatgtat ttgaagcttg
      901 agggtggggg caatcataag tgccagttca agactactta caaggcggcg aaggaaatct
      961 tggaaatgcc tggtgaccac tacatcggtc acagactggt gagaaaaaca gaggggaaca
     1021 ttacagagca agttgaagat gcagtggcac atagcggagg ttgagaccac gaagtgggta
     1081 cctggagcac aagactggcc tcatgggcct tcacttcact gcccgctttc cagtcgggaa
     1141 acctgtcgtg ccagctgcat taacatggtc atagctgttt ccttgcgtat tgggcgctct
     1201 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg ggtaaagcct ggggtgccta
     1261 atgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1321 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1381 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1441 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1501 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1561 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1621 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1681 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1741 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1801 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1861 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1921 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1981 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2041 aatctaaagt atatatgagt aaacttggtc tgacagttat tatttaccca ccactttggt
     2101 aatttcgcct ttcacgtaat gcacaaattc ttccagctga tccgcacggc tggccagacg
     2161 atcttcttcc tggcccagat acgcctgacg cgcttccaga atcaccggct gatactgcgc
     2221 cggcagacgt tccatggccc aatccgccgc cacatctttc ggcgcaattt tgccggtcac
     2281 cgcgctatac caaatacggc tcagggtcag caccacgttg cgttcatcac ccgcccaatc
     2341 cggcgggctg ttccacaggg tcagggtttc gttcagcgct tcaaacaggt cctgttccgg
     2401 caccggatca aacagttctt ccgctgccgg accaaccagc gccacgctat gttcacgcgc
     2461 tttggtcagc agaatggcca gatcgatatc aatggtcgcc ggttcaaaaa tgccggccag
     2521 aatatcgtta cgctgccatt cgccaaactg cagctcacgt ttcgccggat aacgccacgg
     2581 aataatgtca tcatgcacca cgatggtcac ttccaccgca cgcagaattt cgctttcacc
     2641 cgggctcgcg ctggtttcca gcagatcgtt aatcagcgca cgacgggtgg tttcatccag
     2701 acgcacggtc acggtcacca gcagatcaat atcgctatgc ggtttcaggc cgccatccac
     2761 cgcgctgcca tacagatgca cggccagcag ggtcggttcc agatgacgtt caatcacgcc
     2821 caccacttca gacagctggg tgctcacttc cgcaatcacc gcttcacgca taatgttcag
     2881 tttggttttc ggacggctgc cctgctgggt cacatcgttg ctgctccaca gcatcaggca
     2941 acggcccacc gcataacgcg cctgctgttt gctcgcacgc ggcatgctct gcacgccaaa
     3001 aaagcaatca tagcacgcca taaacaccgc caccgcacca ttaccgccgc tacgttcggt
     3061 cagggtacgg ctccaattac ggctacgcat actcttcctt tttcaatatt attgaagcat
     3121 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3181 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
