LOCUS       sequence-26507-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Hackett EA, Esch RK, Maleri S, Errede B
  TITLE     A family of destabilized cyan fluorescent proteins as 
            transcriptional reporters in S. cerevisiae.
  JOURNAL   Yeast. 2006 Apr 15. 23(5):333-49.
  PUBMED    16598699
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Apr 15. 23(5):333-49."
COMMENT     Sequence Label: pNC860
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(580..596)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(580..596)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(593..615)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    604..620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(628..634,635..652,653..658))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    673..694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgggacaact ccngtgaaaa gttcttctcc tttactggat ctctgcatta atgaatcggc
       61 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag
      121 tgagacgggc aacagccaag ctccggatcc ccgggtaccg ggtaataact gatataatta
      181 aattgaagct ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta
      241 gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc
      301 ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga
      361 tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc
      421 atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat
      481 gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt
      541 acccttagta tattctccag agctcgaatt cgtaatcatg tcatagctgt ttcctgtgtg
      601 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      661 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      721 ccagtcggga aacctgtcgt gccagctgca ttatgaatcg gccaacgcgc ggggagaggc
      781 ggtttgnnta ttggggngct cttccnntct cgntcantna annaantcgc tgcgctcgtc
      841 gttcggctgc ggcgaancgg tatcanctca ctcaangngg taatacngtt atccacanaa
      901 tcagggnana
//
