LOCUS       sequence-265070-        6585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse Tcf3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Pereira CF, Chang B, Qiu J, Niu X, Papatsenko D, Hendry CE, Clark 
            NR, Nomura-Kitabayashi A, Kovacic JC, Ma'ayan A, Schaniel C, 
            Lemischka IR, Moore K
  TITLE     Induction of a hemogenic program in mouse fibroblasts.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):205-18. doi: 
            10.1016/j.stem.2013.05.024. Epub 2013 Jun 13.
  PUBMED    23770078
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.05.024"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "205-18"
COMMENT     Sequence Label: pMXs-Tcf3
FEATURES             Location/Qualifiers
     source          1..6585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    642..999
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1058..1474
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1404..1426
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1442..1458
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1484..1858
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1796..1812
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             4054..4523
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4653..4670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4824..5412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4904..4923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5576..6367,6368..6436))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6199..6218
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6437..6541)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             join(6571..6585,1..579)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
       61 actgagaata gaaaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
      121 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
      181 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      241 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
      301 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
      361 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
      421 acccctcact cggcgcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
      481 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
      541 tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg
      601 gagacccctg cccagggacc accgacccac caccgggagg taagctggcc agcaacttat
      661 ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag
      721 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc
      781 ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg cccgacctga
      841 gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc ccctaatagg agggatatgt
      901 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
      961 ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct
     1021 ctgtctgact gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt
     1081 aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga
     1141 tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg
     1201 atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt
     1261 ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt
     1321 ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct
     1381 tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc
     1441 cctttatcca gccctcactc cttctctagg cgcccccata tggccatatg agatcttata
     1501 tggggcaccc ccgccccttg taaacttccc tgaccctgac atgacaagag ttactaacag
     1561 cccctctctc caagctcact tacaggctct ctacttagtc cagcacgaag tctggagacc
     1621 tctggcggca gcctaccaag aacaactgga ccgaccggtg gtacctcacc cttaccgagt
     1681 cggcgacaca gtgtgggtcc gccgacacca gactaagaac ctagaacctc gctggaaagg
     1741 accttacaca gtcctgctga ccacccccac cgccctcaaa gtagacggca tcgcagcttg
     1801 gatacacgcc gcccacgtga aggctgccga ccccgggggt ggaccatcct ctagactgcc
     1861 ggatctagct agttaattaa ggatctcgag ctcaagcttc gaattcatga tgaaccagtc
     1921 tcagagaatg gcacccgtgg gctctgacaa ggaactgagt gacctcctgg acttcagcat
     1981 gatgttcccg ctacctgtgg ccaatgggaa gagccggccc gcctccctcg ggggaaccca
     2041 gtttgcaggc tcaggactgg aggaccgacc cagctcaggc tcctggggca gcagtgacca
     2101 gaacagttct tcctttgacc ctagccggac atacagcgaa ggtgcccact tcagtgactc
     2161 ccacagcagc ctgccgcctt ccacgttcct aggagctggg cttggaggca agggcagtga
     2221 gcggaatgcc tatgccacct ttgggagaga caccagtgtt ggcaccttga gtcaggctgg
     2281 cttcctgcca ggtgagctga gcctcagcag tcccgggcca ctgtccccat cgggcatcaa
     2341 gagcagctcc cagtattacc cctcattccc cagcaaccct cgtcggagag ctgcagatgg
     2401 tggcctggat actcagccga agaaggtccg gaaggttccg cctggtctcc cttcctcggt
     2461 gtatccgccc agctcaggtg acagctacag cagggatgct gcagcctacc cctccgccaa
     2521 gacccccagc agcgcttacc cctccccctt ctacgtggca gatggcagcc tgcacccatc
     2581 agctgagctc tggagtacgc ctagccaggt gggctttggg cccatgctag gtgacggctc
     2641 ttcccctctg ccccttgcac cgggcagcag ctccgtgggc agtggtacct ttgggggcct
     2701 ccagcagcag gatcgcatgg gctaccagct gcatggatct gaggttaatg gctcgctccc
     2761 agctgtatcc agcttttcgg ctgcccctgg cacttacagt gggacttccg gccacacgcc
     2821 ccctgtgagt ggggccgcag ctgaaagcct cctaggcacc cgagggacta cagccagcag
     2881 ctcaggggat gcccttggga aggcactggc ctcgatctac tccccggatc actccagcaa
     2941 taatttctca cctagcccct caacgcctgt gggttcaccc cagggcctgc cagggacatc
     3001 acagtggccc cgggcaggag cgcccagtgc cttatccccc aactacgatg caggtctcca
     3061 tggcctgagc aagatggagg accgcttgga cgaggccatc catgtcctgc gaagccacgc
     3121 tgttggcacc gctagcgatc tccatgggct tttgcctggc catggcgcac tgaccacgag
     3181 cttcaccggc cccatgtcac tgggcgggcg gcatgccggc ctggtcgggg gaagccatcc
     3241 tgaggagggc ctcacaagtg gggccagtct tttgcataac catgccagcc tccccagcca
     3301 gcccagttcc ctccctgacc tctcacagag acctcccgac tcctatagtg gactcgggag
     3361 ggcaggcaca acagcgggtg ccagcgagat caagcgggag gagaaagagg atgaggaaat
     3421 cgcatcagta gccgacgccg aagaggacaa gaaggacctg aaggtcccac gcacgcgcac
     3481 cagcagtaca gatgaggtgc tgtccctgga ggagaaggac ctgagggacc gggagaggcg
     3541 tatggccaat aacgctcggg agcgggtgcg cgtgcgggac attaacgagg ccttccggga
     3601 gctgggccgc atgtgccagc tgcacctcaa gtcggataag gcgcagacca agctgctcat
     3661 cctgcagcag gcggtgcagg tcatcctggg cctggagcag caggtgcgag aacgcaacct
     3721 gaaccccaaa gcagcctgct tgaagcggag ggaggaggag aaggtgtctg gcgtggtcgg
     3781 ggacccacag ctgcccctgt cagccgccca cccgggcctg ggtgaggccc acaacccagc
     3841 cgggcacctg tgaggatccg ttaacgcggc cgccagcaca gtggtcgacg ataaaataaa
     3901 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     3961 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga
     4021 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
     4081 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
     4141 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
     4201 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     4261 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     4321 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
     4381 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     4441 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     4501 tacccgtcag cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc
     4561 ttaagtattt acattaaatg gccatagttg cattaatgaa tcggccaacg cgcggggaga
     4621 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4681 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4741 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4801 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4861 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4921 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4981 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5041 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5101 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5161 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5221 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5281 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5341 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5401 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5461 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5521 ttgcggccgg ccgcaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5581 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5641 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5701 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5761 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5821 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5881 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5941 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6001 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6061 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6121 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6181 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6241 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6301 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6361 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6421 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6481 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6541 gcacatttcc ccgaaaagtg ccacctgcat aatgaaagac cccac
//
