LOCUS       sequence-265073-        3959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate Level 0 S module for N-terminal fluorescent protein 
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Luginbuehl LH, El-Sharnouby S, Wang N, Hibberd JM
  TITLE     Fluorescent reporters for functional analysis in rice leaves.
  JOURNAL   Plant Direct. 2020 Feb 11;4(2):e00188. doi: 10.1002/pld3.188. 
            eCollection 2020 Feb.
  PUBMED    32072132
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-02-11"; volume: "4(2):e00188. doi"; pages: " 
            10.1002/pld3.188. eCollection 2020 Feb"
COMMENT     Sequence Label: pL0M-S-tdTomato-EC18158
FEATURES             Location/Qualifiers
     source          1..3959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1949..1966)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2059..2647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2139..2158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(3832..3936)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggccgcatc actctgtggt ctcaaatggc gagcaagggc gaggaggtta ttaaggaatt
      421 catgaggttc aaagtgcgga tggagggctc catgaatggc catgaatttg agattgaggg
      481 tgagggtgaa ggtaggcctt acgagggaac gcagactgca aagttgaaag tgaccaaggg
      541 agggcccctc ccttttgcgt gggacatcct ctcgccccag ttcatgtatg gtagcaaggc
      601 gtacgtgaag catccggcag acattccgga ctataaaaag ctgtcttttc ctgagggctt
      661 taagtgggag cgggttatga attttgagga cggtggcctt gttacagtga cacaagactc
      721 gagccttcaa gacgggactc tgatatataa ggttaaaatg aggggtacta attttccccc
      781 ggacggcccg gtgatgcaaa agaaaaccat ggggtgggag gcgtcaactg agcgcctcta
      841 tccgcgcgac ggggtgctca agggcgaaat tcaccaggca ttgaaactca aggatggtgg
      901 tcattatctt gtcgaattta aaacgatata catggccaaa aaacccgtgc agttgcccgg
      961 ttattattat gtcgatacta agctcgatat tacatcgcac aacgaggact acacgattgt
     1021 cgaacagtac gaacgcagcg aaggtagaca ccaccttttt ttggggcatg gtactggttc
     1081 gacgggatcg gggtcgtcgg gtacggcatc atcggaggac aacaacatgg cggtgattaa
     1141 agagttcatg aggttcaaag ttaggatgga ggggtctatg aatggacatg aattcgaaat
     1201 tgagggcgag ggggaaggac gcccttatga ggggactcag acagcgaagc ttaaggttac
     1261 taagggagga cctctgccgt ttgcttggga tattctgagc ccgcagttca tgtatggatc
     1321 gaaagcctat gttaaacatc ccgctgacat cccagactac aaaaagcttt catttccgga
     1381 aggtttcaaa tgggaaagag tcatgaattt cgaagatgga ggcctcgtta ctgttacgca
     1441 agacagctct cttcaggacg ggacattgat atataaggtt aagatgcgcg ggaccaactt
     1501 tccgcctgat ggtccggtca tgcaaaaaaa gactatgggc tgggaggcga gtacagaaag
     1561 gctttaccca agagatggcg tgcttaaggg tgaaatacat caagctctta aacttaaaga
     1621 tggagggcat tatctcgttg agttcaaaac gatctatatg gctaagaaac cggttcagct
     1681 gcctggctat tattatgttg ataccaaatt ggatattaca agtcataacg aggactatac
     1741 tatagttgaa cagtatgaaa ggtccgaggg taggcatcac ctgttccttt atggaatgga
     1801 cgaactctat aagggaggtt gagaccacga agtgctgggc ctcatgggcc ttcacttcac
     1861 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaacatggt catagctgtt
     1921 tccttgcgta ttgggcgctc tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1981 gggtaaagcc tggggtgcct aatgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2041 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2461 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2521 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2581 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2641 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2701 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2761 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2821 ttatttaccc accactttgg taatttcgcc tttcacgtaa tgcacaaatt cttccagctg
     2881 atccgcacgg ctggccagac gatcttcttc ctggcccaga tacgcctgac gcgcttccag
     2941 aatcaccggc tgatactgcg ccggcagacg ttccatggcc caatccgccg ccacatcttt
     3001 cggcgcaatt ttgccggtca ccgcgctata ccaaatacgg ctcagggtca gcaccacgtt
     3061 gcgttcatca cccgcccaat ccggcgggct gttccacagg gtcagggttt cgttcagcgc
     3121 ttcaaacagg tcctgttccg gcaccggatc aaacagttct tccgctgccg gaccaaccag
     3181 cgccacgcta tgttcacgcg ctttggtcag cagaatggcc agatcgatat caatggtcgc
     3241 cggttcaaaa atgccggcca gaatatcgtt acgctgccat tcgccaaact gcagctcacg
     3301 tttcgccgga taacgccacg gaataatgtc atcatgcacc acgatggtca cttccaccgc
     3361 acgcagaatt tcgctttcac ccgggctcgc gctggtttcc agcagatcgt taatcagcgc
     3421 acgacgggtg gtttcatcca gacgcacggt cacggtcacc agcagatcaa tatcgctatg
     3481 cggtttcagg ccgccatcca ccgcgctgcc atacagatgc acggccagca gggtcggttc
     3541 cagatgacgt tcaatcacgc ccaccacttc agacagctgg gtgctcactt ccgcaatcac
     3601 cgcttcacgc ataatgttca gtttggtttt cggacggctg ccctgctggg tcacatcgtt
     3661 gctgctccac agcatcaggc aacggcccac cgcataacgc gcctgctgtt tgctcgcacg
     3721 cggcatgctc tgcacgccaa aaaagcaatc atagcacgcc ataaacaccg ccaccgcacc
     3781 attaccgccg ctacgttcgg tcagggtacg gctccaatta cggctacgca tactcttcct
     3841 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3901 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
