LOCUS       sequence-265079-        4011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC38A1
FEATURES             Location/Qualifiers
     source          1..4011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2136..2235)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2252..2271)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2253..2271)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2276..2292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2276..2292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2394..2413)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2405..3214
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2472..2491)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3361..3949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3850..3869
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgatgc acttcaagag cggcctggag ctgaccgagc tgcagaatat
      721 gacagtgcct gaggacgata acatctccaa tgattctaac gacttcaccg aggtggagaa
      781 tggccagatc aactctaagt ttatcagcga tagggagagc cggagatccc tgacaaactc
      841 ccacctggag aagaagaagt gcgacgagta catcccaggc accacatccc tgggcatgag
      901 cgtgttcaac ctgagcaacg ccatcatggg atccggaatc ctgggactgg ccttcgccct
      961 ggcaaatacc ggcatcctgc tgtttctggt gctgctgacc tccgtgacac tgctgtctat
     1021 ctacagcatc aacctgctgc tgatctgctc taaggagaca ggctgtatgg tgtatgagaa
     1081 gctgggcgag caggtgttcg gcaccaccgg caagttcgtg atctttggcg ccacctccct
     1141 gcagaataca ggcgccatgc tgtcttacct gtttatcgtg aagaacgagc tgccatctgc
     1201 catcaagttc ctgatgggca aggaggagac ctttagcgcc tggtacgtgg atggccgcgt
     1261 gctggtggtc atcgtgacat tcggcatcat cctgcccctg tgcctgctga agaatctggg
     1321 ctacctgggc tataccagcg gcttctctct gagctgcatg gtgttctttc tgatcgtggt
     1381 catctacaag aagtttcaga tcccctgtat cgtgcctgag ctgaattcca ccatctctgc
     1441 caatagcaca aacgccgata cctgcacacc taagtacgtg acctttaact ccaagacagt
     1501 gtatgccctg ccaaccatcg ccttcgcctt cgtgtgccac ccatctgtgc tgcccatcta
     1561 tagcgagctg aaggaccggt cccagaagaa gatgcagatg gtgtccaata tctctttctt
     1621 tgccatgttc gtgatgtact ttctgaccgc catcttcggc tacctgacct tctacgataa
     1681 cgtgcagtct gacctgctgc acaagtatca gagcaaggac gatatcctga tcctgaccgt
     1741 gagactggcc gtgatcgtgg ccgtgatcct gaccgtgccc gtgctgttct ttacagtgag
     1801 gagctccctg ttcgagctgg ccaagaagac aaagtttaat ctgtgccgcc acaccgtggt
     1861 gacatgtatc ctgctggtgg tcatcaacct gctggtcatc ttcatcccca gcatgaagga
     1921 catctttggc gtggtgggcg tgacctccgc caacatgctg atcttcatcc tgccttctag
     1981 cctgtacctg aagatcaccg accaggatgg cgacaagggc acacagcgga tctgggccgc
     2041 cctgttcctg ggactgggcg tgctgtttag cctggtgtcc atccctctgg tcatctacga
     2101 ttgggcatgc tcctctagct ccgacgaggg acacgaccca gctttcttgt acaaagttgg
     2161 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     2221 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     2281 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     2341 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     2401 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     2461 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     2521 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     2581 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     2641 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     2701 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     2761 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     2821 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     2881 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     2941 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     3001 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     3061 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     3121 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     3181 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     3241 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
     3301 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3361 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3421 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3481 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3541 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3601 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3661 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3721 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3781 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3841 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3901 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3961 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
