LOCUS       sequence-26509-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Hackett EA, Esch RK, Maleri S, Errede B
  TITLE     A family of destabilized cyan fluorescent proteins as 
            transcriptional reporters in S. cerevisiae.
  JOURNAL   Yeast. 2006 Apr 15. 23(5):333-49.
  PUBMED    16598699
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Apr 15. 23(5):333-49."
COMMENT     Sequence Label: pNC862
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(161..388)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(797..820)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gaattgggac aactccagtg aaaagttctt ctcctttact ggatctctgc attaatgaat
       61 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
      121 ccagtgagac gggcaacagc caagctccgg atccgtgcat accacctctt agccttagca
      181 caagatgtaa ggtggactcc ttctgaatgt tgtaatcaga cagcgttcta ccgtcttcta
      241 gctgcttacc ggcaaagatc aatctttgtt gatctggagg gataccttcc ttgtcttgaa
      301 ttttcgactt aacgttgtcg atggtatcgg aagattcaac ttccaatgtt atggttttac
      361 cggtcaaagt cttgacgaaa atctgcataa tctattagtt aaagtaaagt gggagggaga
      421 gttcgagagt tcttttcgta ttgtagtaga tagaagatat acttatcctt atccgtacag
      481 ttcttgctct ttctgaagta gcactttcgg aagaagaaag ataagcaggc gaattgatcc
      541 ggtaatttag tgtgtgtatt tgtgtttgcg tgtctataga agtatagtaa tttatgctac
      601 aaaggaccta atgtataagg aaagaatatt tagagaaaag aagaaaacaa gagttttata
      661 tacatacaga gcacatgcat gccatatgat catgtgtcgt cgcacacata tatatatgcc
      721 tgtatgtgtc agcactaaag ttgcctggcc atccacgcta tatacacgcc tggcagatcc
      781 ggggtttttt ctccttgacg ttaaagtata gaggtatatt aacaattttt tgttgatact
      841 tttattacat ttgaataa
//
