LOCUS       sequence-26512-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Hackett EA, Esch RK, Maleri S, Errede B
  TITLE     A family of destabilized cyan fluorescent proteins as 
            transcriptional reporters in S. cerevisiae.
  JOURNAL   Yeast. 2006 Apr 15. 23(5):333-49.
  PUBMED    16598699
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Apr 15. 23(5):333-49."
COMMENT     Sequence Label: pNC866
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..232)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(641..664)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gttcnccncc tcttagcctt agcacaagat gtaaggtgga ctccttctga atgttgtaat
       61 cagacagcgt tctaccgtct tctagctgct taccggcaaa gatcaatctt tgttgatctg
      121 gagggatacc ttccttgtct tgaattttcg acttaacgtt gtcgatggta tcggaagatt
      181 caacttccaa tgttatggtt ttaccggtca aagtcttgac gaaaatctgc ataatctatt
      241 agttaaagta aagtgggagg gagagttcga gagttctttt cgtattgtag tagatagaag
      301 atatacttat ccttatccgt acagttcttg ctctttctga agtagcactt tcggaagaag
      361 aaagataagc aggcgaattg atccggtaat ttagtgtgtg tatttgtgtt tgcgtgtcta
      421 tagaagtata gtaatttatg ctacaaagga cctaatgtat aaggaaagaa tatttagaga
      481 aaagaagaaa acaagagttt tatatacata cagagcacat gcatgccata tgatcatgtg
      541 tcgtcgcaca catatatata tgcctgtatg tgtcagcact aaagttgcct ggccatccac
      601 gctatataca cgcctggcag atccggggtt ttttctcctt gacgttaaag tatagaggta
      661 tattaacaat tttttgttga tacttttatt acatttgaat aagaagtaat acaaaccgaa
      721 aatgttgaaa gtattagtta aagtggttat gcagtttttg catttatata tctgttaata
      781 gatcaaaaat catcgcttcg ctgantaatt accccagaaa tnanggntaa aaaactaatc
      841 gcattatcat cctatggntg ttaatttgat tcgttcattt gaangtttgn ggggcca
//
