LOCUS       sequence-26514-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Hackett EA, Esch RK, Maleri S, Errede B
  TITLE     A family of destabilized cyan fluorescent proteins as 
            transcriptional reporters in S. cerevisiae.
  JOURNAL   Yeast. 2006 Apr 15. 23(5):333-49.
  PUBMED    16598699
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Apr 15. 23(5):333-49."
COMMENT     Sequence Label: pNC891
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..437
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     complement(495..523)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tacgctgcag gtcgactatt gaagtaataa taggcgcatg caacttcttt tctttttttt
       61 tcttttctct ctcccccgtt gttgtctcac catatccgca atgacaaaaa aatgatggaa
      121 gacactaaag gaaaaaatta acgacaaaga cagcaccaac agatgtcgtt gttccagagc
      181 tgatgagggg tatctcgaag cacacgaaac tttttccttc cttcattcac gcacactact
      241 ctctaatgag caacggtata cggccttcct tccagttact tgaatttgaa ataaaaaaaa
      301 gtttgctgtc ttgctatcaa gtataaatag acctgcaatt attaatcttt tgtttcctcg
      361 tcattgttct cgttcccttt cttccttgtt tctttttctg cacaatattt caagctatac
      421 caagcataca atcaactatc tcatatacaa tgcccgggtt aattaacagt aaaggagaag
      481 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      541 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      601 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt
      661 atggtctaaa atgctttgct agatacccag atcatatgaa acggcatgac tttttcaaga
      721 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
      781 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
      841 aaggnattga ttttaaagaa nanggaaaca ttctng
//
