LOCUS       sequence-265156-        7020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mamamlian expression of 3xHA tagged reptin, N-terminal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Venteicher AS, Meng Z, Mason PJ, Veenstra TD, Artandi SE
  TITLE     Identification of ATPases pontin and reptin as telomerase components
            essential for holoenzyme assembly.
  JOURNAL   Cell. 2008 Mar 21;132(6):945-57. doi: 10.1016/j.cell.2008.01.019.
  PUBMED    18358808
REFERENCE   2  (bases 1 to 7020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2008.01"; journalName: "Cell"; date: "2008-03-21- 
            21"; volume: "132"; issue: "6"; pages: "945-57"
COMMENT     Sequence Label: pCDNA-3xHA-Reptin
FEATURES             Location/Qualifiers
     source          1..7020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(438..458)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        443..772
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      623..758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     685..704
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             839..1633
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(893..912)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1503..1522
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1809..1930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1846..1865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1900..1919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1979..1995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1979..1995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1992..2014)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2003..2019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2027..2033,2034..2051,2052..2057))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2072..2093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2210..2227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2381..2969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2461..2480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3140..3931,3932..4000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3763..3782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4001..4105)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4180..4199)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4371..4750
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4751..4954
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4905..4925
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4999..5018
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4999..5017
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5096..5105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5117..5143
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5147..5173
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5177..5203
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6660..6689
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6705..6722
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6745..6762)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6751..6975
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
      481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      541 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      601 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
      721 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
      781 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
      841 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      901 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      961 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     1021 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     1081 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     1141 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     1201 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     1261 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     1321 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     1381 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1441 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1501 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     1561 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1621 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc
     1681 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     1741 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     1801 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     1861 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     1921 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     1981 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2041 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     2101 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     2161 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     2221 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2821 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3121 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3181 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3241 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3301 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3361 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3421 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3481 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3541 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3601 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3661 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3721 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3781 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3841 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3901 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3961 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4021 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     4081 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     4141 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     4201 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     4261 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     4321 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     4381 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     4441 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     4501 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     4561 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     4621 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     4681 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     4741 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     4801 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     4861 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     4921 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
     4981 ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg gctagttaag
     5041 cttggtaccg agctcggatc cactagtcca gtgtggtgga attctgcaga taaacgccac
     5101 catggggcaa caaggatacc cgtatgatgt tccggattac gctggctacc catacgacgt
     5161 cccagactac gctggctacc catacgacgt cccagactac gctcaaggag tgcggccgcg
     5221 ggggcgcgcc gcaaccgtta cagccacaac caaagtcccg gagatccgtg atgtaacaag
     5281 gattgagcga atcggtgccc actcccacat ccggggactg gggctggacg atgccttgga
     5341 gcctcggcag gcttcgcaag gcatggtggg tcagctggcg gcacggcggg cggctggcgt
     5401 ggtgctggag atgatccggg aagggaagat tgccggtcgg gcagtcctta ttgctggcca
     5461 gccgggcacg gggaagacgg ccatcgccat gggcatggcg caggccctgg gccctgacac
     5521 gccattcaca gccatcgccg gcagtgaaat cttctccctg gagatgagca agaccgaggc
     5581 gctgacgcag gccttccggc ggtccatcgg cgttcgcatc aaggaggaga cggagatcat
     5641 cgaaggggag gtggtggaga tccagattga tcgaccagca acagggacgg gctccaaggt
     5701 gggcaaactg accctcaaga ccacagagat ggagaccatc tacgatttag gaacgaagat
     5761 gattgagtcc ctgaccaagg acaaggtcca ggccggggac gtgatcacca tcgacaaggc
     5821 gacgggcaag atctccaagc tgggccgctc cttcacacgc gcccgcgact acgacgctat
     5881 gggctcccag accaagttcg tgcagtgccc agatggggag ctccagaaac gcaaggaggt
     5941 ggtgcacacc gtgtccctgc acgagatcga cgtcatcaac tctcgcaccc agggcttcct
     6001 ggcgctcttc tcaggtgaca caggggagat caagtcagaa gtccgtgagc agatcaatgc
     6061 caaggtggct gagtggcgcg aggagggcaa ggcggagatc atccctggag tgctgttcat
     6121 cgacgaggtc cacatgctgg acatcgagag cttctccttc ctcaaccggg ccctggagag
     6181 tgacatggcg cctgtcctga tcatggccac caaccgtggc atcacgcgaa tccggggcac
     6241 cagctaccag agccctcacg gcatccccat agacctgctg gaccggctgc ttatcgtctc
     6301 caccaccccc tacagcgaga aagacacgaa gcagatcctc cgcatccggt gcgaggaaga
     6361 agatgtggag atgagtgagg acgcctacac ggtgctgacc cgcatcgggc tggagacgtc
     6421 actgcgctac gccatccagc tcatcacagc tgccagcttg gtgtgccgga aacgcaaggg
     6481 tacagaagtg caggtggatg acatcaagcg ggtctactca ctcttcctgg acgagtcccg
     6541 ctccacgcag tacatgaagg agtaccagga cgccttcctc ttcaacgaac tcaaaggcga
     6601 gaccatggac acctcctgat taattaagtc tagagtcgag tctagagggc ccgcggttcg
     6661 aacaaaaact catctcagaa gaggatctga atatgcatac cggtcatcat caccatcacc
     6721 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6781 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6841 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6901 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6961 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
//
