LOCUS       sequence-265164-        1573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of F-actin targeted ultrasensitive hydrogen 
            peroxide indicator HyPer7 fused with LifeAct peptide for optical 
            imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Pak VV, Ezerina D, Lyublinskaya OG, Pedre B, Tyurin-Kuzmin PA, 
            Mishina NM, Thauvin M, Young D, Wahni K, Martinez Gache SA, 
            Demidovich AD, Ermakova YG, Maslova YD, Shokhina AG, Eroglu E, Bilan
            DS, Bogeski I, Michel T, Vriz S, Messens J, Belousov VV
  TITLE     Ultrasensitive Genetically Encoded Indicator for Hydrogen Peroxide 
            Identifies Roles for the Oxidant in Cell Migration and Mitochondrial
            Function.
  JOURNAL   Cell Metab. 2020 Mar 3;31(3):642-653.e6. doi: 
            10.1016/j.cmet.2020.02.003.
  PUBMED    32130885
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2020.02"; journalName: "Cell Metab"; date: 
            "2020-03-3- 3"; volume: "31"; issue: "3"; pages: "642-653.e6"
COMMENT     Sequence Label: pLifeAct-HyPer7
FEATURES             Location/Qualifiers
     source          1..1573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..79
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     primer_bind     706..727
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(836..857)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1097..1116)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca agaagttcga
       61 gtccatctcc aaggaggagg gggatccacc ggtcatgcac ctggctaatg aggagcaaaa
      121 cgaattggaa ggtgctttta agctgggctt gatctttacc gtcgccccgt acttgctgcc
      181 taagttgatc gtcagcctgc gccgcaccgc cccgaagatg cctttgatgt tggaggagaa
      241 ttacacccac acccttaccg agtcactgaa acgcggcgac gttgacgcca tcattgtggc
      301 agagccgttt caagagccgg gtattgtgac tgaacctttg tatgacgagc cgtttttcgt
      361 tatcgttccg aagggccata gcttcgagga gttggatgcc gttagccctc gcatgttggg
      421 cgaagaacaa gttctgcttc tgaccgaggg caactgtatg cgtgaccagg tgcttagctc
      481 cgccggcttc aacagcaaca acgtctatat catggccgac aagcagaaga acggcatcaa
      541 ggccaacttc aagatccgcc acaacgtcga ggacggcagc gtgcagctcg ccgaccacta
      601 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      661 cttccagtcc gtcctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      721 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaacg tggatggcgg
      781 tagcggtggc accggcagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      841 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
      901 tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc
      961 ctggcccacc ctcgtgacca ccctcagcta cggcctgaag tgcttcgccc gctaccccga
     1021 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     1081 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1141 cgacaccctg gtgaaccgca tcgagctgaa gggcatcggc ttcaaggagg acggcaacat
     1201 cctggggcac aagctggagt acaacggccc cagttgcagc gagcttgccg ccaaacagcg
     1261 cattcagggt ctcaccaaca cccttcaagg ctcaagcatc aataccatcc gccacatggt
     1321 tgccagtggc ttggcaatca gcgtcttgcc tgccactgcc cttaccgaaa acgaccacat
     1381 gctgtttagc attatcccgt ttgaaggcac cccgcctagc cgccgcgttg tcctggccta
     1441 tcgccgcaac tttgtccgcc ctaaggccct ttcagccatg aaagccgcaa ttatgcaaag
     1501 ccaactgcat ggtgttagct tcatctgcga ttgagcggcc gcgactctag atcataatca
     1561 gccataccac att
//
