LOCUS       sequence-265168-        1290 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual fluorescent reporter for liver stage expression in Plasmodium 
            cynomolgi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Voorberg-van der Wel AM, Zeeman AM, Nieuwenhuis IG, van der Werff 
            NM, Klooster EJ, Klop O, Vermaat LC, Kumar Gupta D, Dembele L, 
            Diagana TT, Kocken CHM
  TITLE     A dual fluorescent Plasmodium cynomolgi reporter line reveals in 
            vitro malaria hypnozoite reactivation.
  JOURNAL   Commun Biol. 2020 Jan 3;3:7. doi: 10.1038/s42003-019-0737-3. 
            eCollection 2020.
  PUBMED    31909199
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-01-3"; volume: "3:7. doi"; pages: " 
            10.1038/s42003-019-0737-3. eCollection 2020"
COMMENT     Sequence Label: pCyCEN_Lisp2mCherry_hsp70_GFP
FEATURES             Location/Qualifiers
     source          1..1290
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..714
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(152..170)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     420..439
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             718..771
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             772..1284
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
ORIGIN
        1 agatctatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
       61 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      121 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
      181 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
      241 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      301 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      361 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      421 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      481 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
      541 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
      601 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
      661 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagggagaa
      721 ggaagaggaa gtttattaac atgtggagat gtagaagaaa atccaggacc agtcttcaca
      781 ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt
      841 gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa
      901 aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat
      961 gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct
     1021 gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt
     1081 acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc
     1141 aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg
     1201 atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg
     1261 ctgtgcgaac gcattctggc gtaagatatc
//
