LOCUS       sequence-265171-        7026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NOTE: This plasmid contains 1xHA-mASL, not 3xHA-mASL..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7026)
  TITLE     Peter Murray lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1-3xHA-mASL
FEATURES             Location/Qualifiers
     source          1..7026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(438..458)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        443..772
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      623..758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     685..704
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             821..1846
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             821..1846
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    1976..2097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2013..2032)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2067..2086
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2146..2162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2146..2162)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2159..2181)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2170..2186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2194..2200,2201..2218,2219..2224))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2239..2260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2377..2394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2548..3136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2628..2647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3307..4098,4099..4167))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3930..3949
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4168..4272)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4347..4366)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4538..4917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4918..5121
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5072..5092
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5166..5185
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5166..5184
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5295..5321
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6751..6768)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6757..6981
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
      481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      541 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      601 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
      721 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
      781 gggagcttgt atatccattt tcggatctga tcagcacgtg atgaaaaagc ctgaactcac
      841 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
      901 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
      961 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     1021 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     1081 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     1141 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     1201 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     1261 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     1321 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     1381 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     1441 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     1501 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     1561 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     1621 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     1681 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     1741 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     1801 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tgctacgaga
     1861 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     1921 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     1981 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2041 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2101 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     2161 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     2221 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     2281 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     2341 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     2401 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     2461 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2521 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2581 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2641 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2701 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2761 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2821 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2881 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2941 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     3001 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3061 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3121 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3181 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3241 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3301 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     3361 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     3421 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     3481 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3541 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3601 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     3661 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3721 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3781 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3841 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3901 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3961 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4021 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4081 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4141 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4201 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4261 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     4321 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4381 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     4441 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     4501 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
     4561 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     4621 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     4681 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     4741 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     4801 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     4861 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     4921 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     4981 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     5041 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     5101 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
     5161 gaaattaata cgactcacta tagggagacc caagctggct agcgtttaaa cttaagcttg
     5221 gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg
     5281 gcggccgcac catgtaccct tacgacgtac ctgactacgc agcatcagag agcgggaagc
     5341 tatggggtgg ccggtttgtg ggtgctgtcg accccatcat ggagaagttc aactcatcta
     5401 tctcctatga ccggcatctg tggaatgtgg atgtgcaggg gagcaaggcc tacagcaggg
     5461 gcctggagaa ggcggggctt ctcaccaaag ccgagatgca gcagatactg caagggttgg
     5521 acaaggttgc tgaagagtgg gcccaaggca ccttcaaatt acatcctaat gatgaagaca
     5581 tccacacagc caacgagcgg cgcctgaagg aactcatcgg tgaagctgca gggaagctac
     5641 acacaggacg aagtcgcaac gaccaggtgg tcacggacct caggctgtgg atgaggcaga
     5701 cctgctcaaa actctccgcc ctcctccggg tgctcattgg aaccatggtc gaccgggcag
     5761 aggcggaacg tgatgtcctc ttcccagggt acacacactt gcagagagct cagcccattc
     5821 gctggagcca ctggatcctg agccacgctg ttgcgctgac acgagattca gagagactct
     5881 tggaggtgca gaagcggatc aatgtcttgc cactggggag tggggccatt gcaggcaacc
     5941 ctctgggtgt ggaccgggag ctactccgtg cagaactgaa ctttggggcc atcactctca
     6001 acagcatgga tgccaccagt gagagagact ttgtggctga gttcctgttt tgggcttctc
     6061 tgtgtatgac ccacctcagc aggatggcag aggacctaat tctctatggt accaaggaat
     6121 tcagctttgt gcaactctca gatgcctaca gcaccggaag cagcttgatg ccccagaaga
     6181 aaaacccaga cagcctggag ctgatccgga gcaaggcggg ccgagtgttt ggacggtgtg
     6241 ccggactcct gatgaccctc aagggacttc caagcaccta caacaaggac ttacaggaag
     6301 acaaggaggc tgtgtttgaa gtgtctgaca ccatgatagc tgtcctccaa gtggccactg
     6361 gagtcatctc tacattgcag attcatcgtg agaacatgaa acaggcgctc agccctgaca
     6421 tgctggccac ggacctcgcc tactacctgg tccgcaaagg gatgccattc cgccaggccc
     6481 acgaggcctc agggaaagct gtgttcatgg cagagactaa aggagtggct ctcaacctgc
     6541 tgtcgctaca ggagctgcag accatcagtc ccctgttctc aggtgatgtg agtcacgtgt
     6601 gggactacag tcacagcgtg gaacagtaca gtgccttggg tggcacagcg aaatccagtg
     6661 tcgaatggca gatcaggcag gtgcgggccc tactgcaagc ccaggagccc tagctcgagt
     6721 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     6781 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6841 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6901 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6961 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     7021 atcccc
//
