LOCUS       sequence-26518-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Hackett EA, Esch RK, Maleri S, Errede B
  TITLE     A family of destabilized cyan fluorescent proteins as 
            transcriptional reporters in S. cerevisiae.
  JOURNAL   Yeast. 2006 Apr 15. 23(5):333-49.
  PUBMED    16598699
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Apr 15. 23(5):333-49."
COMMENT     Sequence Label: pNC952
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(111..338)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(747..770)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     gap             849..860
                     /estimated_length=12
ORIGIN
        1 attgggacaa ctccagtgaa aagttcttct cctttactcg tattgggcgc cagggtggtt
       61 tttcttttca ccagtgagac gggcaacagc caagctccgg atccgtgcat accacctctt
      121 agccttagca caagatgtaa ggtggactcc ttctgaatgt tgtaatcaga cagcgttcta
      181 ccgtcttcta gctgcttacc ggcaaagatc aatctttgtt gatctggagg gataccttcc
      241 ttgtcttgaa ttttcgactt aacgttgtcg atggtatcgg aagattcaac ttccaatgtt
      301 atggttttac cggtcaaagt cttgacgaaa atctgcataa tctattagtt aaagtaaagt
      361 gggagggaga gttcgagagt tcttttcgta ttgtagtaga tagaagatat acttatcctt
      421 atccgtacag ttcttgctct ttctgaagta gcactttcgg aagaagaaag ataagcaggc
      481 gaattgatcc ggtaatttag tgtgtgtatt tgtgtttgcg tgtctataga agtatagtaa
      541 tttatgctac aaaggaccta atgtataagg aaagaatatt tagagaaaag aagaaaacaa
      601 gagttttata tacatacaga gcacatgcat gccatatgat catgtgtcgt cgcacacata
      661 tatatatgcc tgtatgtgtc agcactaaag ttgcctggnc atccacgcta tatacacgcc
      721 tggcagatcc ggggtttttt ctccttgacg ttaaagtata gaggtatatt aacaattttt
      781 tgttgatact tttattacat ttgaataana agtaatacaa accgaaaang ntgaaagnat
      841 tagttnaann nnnnnnnnnn tttttgnatt tatatatcng tnnnananca aaaanc
//
