LOCUS       sequence-265186-        1971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Low Phosphate inducible matB (malonyl-CoA synthetase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, 
            Wang R, Lynch MD
  TITLE     Scalable, two-stage, autoinduction of recombinant protein expression
            in E. coli utilizing phosphate depletion.
  JOURNAL   Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440.
  PUBMED    32441815
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2020 May 22. doi: 10.1002/bit.27440."
COMMENT     Sequence Label: pCDF-yibDp-matB
FEATURES             Location/Qualifiers
     source          1..1971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      12..83
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      99..126
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             409..426
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1168..1179)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgcccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
       61 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      121 tctgcgttta tacacagcta acaccacgtc gtccctatct gctgccctag gtctatgagt
      181 ggttgctgga taacgtgcgt aattgtgctg atctcttata tagctgctct cattatctct
      241 ctaccctgaa gtgactctct cacctgtaaa aataatatct cacaggctta atagtttctt
      301 aatacaaagc ctgtaaaacg tcaggataac ttctatattc agggagacca caacggtttc
      361 cctctacaaa taattttgtt taactttgga aaaaggagat ataccatgca tcatcatcat
      421 catcactcca accatttatt cgatgcgatg cgtgccgcgg cgcctgggaa tgcgccgttc
      481 atccgcatcg acaatacacg cacctggacc tacgacgatg cctttgcctt gagtggtcgc
      541 attgcgtcag ctatggacgc actcggcatt cgcccgggag accgcgtggc cgtgcaggtg
      601 gagaaatctg ccgaagcact gattctgtat cttgcgtgtc tgcgtagcgg tgccgtatat
      661 ttgccactga acactgctta tacacttgcg gaactggact actttattgg tgatgccgaa
      721 ccgcgcctcg ttgttgtagc gtcatccgcc cgtgcaggtg tggaaaccat tgcgaaaccg
      781 cgcggggcca ttgtagaaac tctggatgca gctggcagcg gaagcttgct ggacttggcg
      841 cgcgatgagc ctgctgattt cgtggacgct agtcgctcgg cggacgatct ggcggcaatt
      901 ctttatacaa gtgggacgac agggcgttct aagggggcaa tgctgacaca cggcaacctg
      961 ctttctaacg cgcttacatt gcgtgatttc tggcgtgtaa ccgcaggcga tcgtctgatt
     1021 catgcgttac cgatttttca tacacatggc ctgtttgtcg ctactaatgt cacattactg
     1081 gccggggcct ctatgtttct gttaagcaaa tttgatccgg aagagatcct gtctttgatg
     1141 ccgcaggcta ccatgctgat gggcgtaccg accttctatg ttcgcttgct gcaatcaccg
     1201 cgcctggata aacaggcagt agcgaatatt cgcctgttta ttagtgggtc cgcaccactg
     1261 ctggcagaga cacacactga atttcaagcg cgtaccggcc atgccattct ggaacgctac
     1321 ggaatgaccg agaccaacat gaacacctca aatccgtatg agggtaaacg tattgcgggt
     1381 actgtgggct ttcctctccc ggatgtcact gttcgtgtta ccgacccggc aaccggtctc
     1441 gccttacctc cggaacagac gggaatgatc gaaattaaag gtccgaacgt gtttaagggc
     1501 tattggcgca tgcccgagaa gaccgctgcc gaattcaccg ccgatggttt ctttatcagt
     1561 ggtgatttag gtaaaatcga tcgcgatgga tacgttcata ttgtggggcg cgggaaagat
     1621 ctggttattt caggaggcta taatatttat ccgaaagaag ttgaggggga aattgaccag
     1681 attgaagggg tggttgaatc agcagtgatc ggcgttccgc acccggattt tggtgaaggt
     1741 gtcacagcgg ttgtggttcg caaaccaggg gcggctctgg atgaaaaagc gatcgtctct
     1801 gctctgcagg accgtctggc tcgttataaa caaccgaaac gcatcatttt tgctgaagat
     1861 ctgccgcgta acacaatggg taaagtccag aaaaacatct tgcgccagca gtatgcagac
     1921 ttatatactc gtacgtagta agacgaattc tctagatatc gctcaatact g
//
