LOCUS       sequence-265189-       10466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expession of Flag-CtlP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 10466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pCW-Flag-CtIP
FEATURES             Location/Qualifiers
     source          1..10466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    547..571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             581..604
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(3339..3363)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3381..3398
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3408..3918
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3837..3855
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3928..4524
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3928..3947)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4424..4444
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4525..4578
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4579..5196,5197..5202,5203..5238,5239..5241,5242..5277,
                     5278..5280,5281..5316,5317..5325)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5365..5385)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5783..5794)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5999..6232
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6304..6425
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6341..6360)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6395..6414
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6465..6600
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6600..6671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6672..6740,6741..7532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6890..6909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7703..8291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8192..8211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8445..8462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8579..8600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8615..8620,8621..8638,8639..8645)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8653..8669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8658..8680
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8677..8693
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8677..8693
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8712..8732
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8714..8732
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8760..8986
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8987..9167
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9214..9339
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9832..10065
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10250..10294
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagcaggc taccaccatg gactacaaag acgatgacga
      601 taaagccgcc agtttaaacg gcgcgccatt aattaaggat ccaatgaaca tctcgggaag
      661 cagctgtgga agccctaact ctgcagatac atctagtgac tttaaggacc tttggacaaa
      721 actaaaagaa tgtcatgata gagaagtaca aggtttacaa gtaaaagtaa ccaagttgaa
      781 gcaggaacga atcttagatg cacaaagact agaagaattc ttcaccaaaa atcaacagct
      841 gagggaacag cagaaagtcc ttcatgaaac cattaaagtt ttagaagatc ggttaagagc
      901 aggcttatgt gatcgctgtg cagtaactga agaacatatg cggaaaaaac agcaagagtt
      961 tgaaaatatc cggcagcaga atcttaaact tattacagaa cttatgaatg aaaggaatac
     1021 tctacaggaa gaaaataaaa agctttctga acaactccag cagaaaattg agaatgatca
     1081 acagcatcaa gcagctgagc ttgaatgtga ggaagacgtt attccagatt caccgataac
     1141 agccttctca ttttctggcg ttaaccggct acgaagaaag gagaaccccc atgtccgata
     1201 catagaacaa acacatacta aattggagca ctctgtgtgt gcaaatgaaa tgagaaaagt
     1261 ttccaagtct tcaactcatc cacaacataa tcctaatgaa aatgaaattc tagtagctga
     1321 cacttatgac caaagtcaat ctccaatggc caaagcacat ggaacaagca gctatacccc
     1381 tgataagtca tcttttaatt tagctacagt tgttgctgaa acacttggac ttggtgttca
     1441 agaagaatct gaaactcaag gtcccatgag ccccattggt gatgagctct accactgtct
     1501 ggaaggaaat cacaagaaac agccttttga ggaatctaca agaaatactg aagatagttt
     1561 aagattttca gattctactt caaagactcc tcctcaagaa gaattaccta ctcgagtgtc
     1621 atctcctgta tttggagcta cctctagtat caaaagtggt ttagatttga atacaagttt
     1681 gtccccttct cttttacagc ctgggaaaaa aaaacatctg aaaacactcc cttttagcaa
     1741 cacttgtata tctagattag aaaaaactag atcaaaatct gaagatagtg cccttttcac
     1801 acatcacagt cttgggtctg aagtgaacaa gatcattatc cagtcatcta ataaacagat
     1861 acttataaat aaaaatataa gtgaatccct aggtgaacag aataggactg agtacggtaa
     1921 agattctaac actgataaac atttggagcc cctgaaatca ttgggaggcc gaacatccaa
     1981 aaggaagaaa actgaggaag aaagtgaaca tgaagtaagc tgcccccaag cttcttttga
     2041 taaagaaaat gctttccctt ttccaatgga taatcagttt tccatgaatg gagactgtgt
     2101 gatggataaa cctctggatc tgtctgatcg attttcagct attcagcgtc aagagaaaag
     2161 ccaaggaagt gagacttcta aaaacaaatt taggcaagtg actctttatg aggctttgaa
     2221 gaccattcca aagggctttt cctcaagccg taaggcctca gatggcaact gcacgttgcc
     2281 caaagattcc ccaggggagc cctgttcaca ggaatgcatc atccttcagc ccttgaataa
     2341 atgctctcca gacaataaac catcattaca aataaaagaa gaaaatgctg tctttaaaat
     2401 tcctctacgt ccacgtgaaa gtttggagac tgagaatgtt ttagatgaca taaagagtgc
     2461 tggttctcat gagccaataa aaatacaaac caggtcagac catggaggat gtgaacttgc
     2521 atcagttctt cagttaaatc catgtagaac tggtaaaata aagtctctac aaaacaacca
     2581 agatgtatcc tttgaaaata tccagtggag tatagatccg ggagcagacc tttctcagta
     2641 taaaatggat gttactgtaa tagatacaaa ggatggcagt cagtcaaaat taggaggaga
     2701 gacagtagac atggactgta cattggttag tgaaaccgtt ctcttaaaaa tgaagaaaca
     2761 agagcagaag ggagaaaaaa gttcaaatga agaaagaaaa atgaatgata gcttggaaga
     2821 tatgtttgat cggacaacac atgaagagta tgaatcctgt ttggcagaca gtttctccca
     2881 agcagcagat gaagaggagg aattgtctac tgccacaaag aaactacaca ctcatggtga
     2941 taaacaagac aaagtcaagc agaaagcgtt tgtggagccg tattttaaag gtgatgaaag
     3001 agagactagc ttgcaaaatt ttcctcatat tgaggtggtt cggaaaaaag aggagagaag
     3061 aaaactgctt gggcacacgt gtaaggaatg tgaaatttat tatgcagata tgccagcaga
     3121 agaaagagaa aagaaattgg cttcctgctc aagacaccga ttccgctaca ttccacccaa
     3181 cacaccagag aatttttggg aagttggttt tccttccact cagacttgta tggaaagagg
     3241 ttatattaag gaagatcttg atccttgtcc tcgtccaaaa agacgtcagc cttacaacgc
     3301 aatattttct ccaaaaggca aggagcagaa gacataggac ccagctttct tgtacaaagt
     3361 ggtttagtaa tgaaccggtc caccaccacc accaccacta aggatccggg gttggggttg
     3421 cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc
     3481 gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc
     3541 acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta
     3601 agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt
     3661 ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga
     3721 tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg
     3781 gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt
     3841 tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc
     3901 accgacctct ctccccagca attcaccatg accgagtaca agcccacggt gcgcctcgcc
     3961 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     4021 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     4081 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     4141 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     4201 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     4261 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     4321 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     4381 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     4441 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     4501 tgcatgaccc gcaagcccgg tgccgaaggt agaggttctc tcctcacttg tggtgatgtt
     4561 gaagaaaacc ctggtccaat gtctagactg gacaagagca aagtcataaa cggagctctg
     4621 gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg
     4681 ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc
     4741 ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca
     4801 tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc
     4861 gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa
     4921 aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg
     4981 tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca
     5041 aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt
     5101 gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata
     5161 tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat
     5221 tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct
     5281 gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag taaggatcga
     5341 tccaagatat cgtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5401 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5461 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5521 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5581 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5641 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5701 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5761 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5821 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5881 cctttgggcc gcctccccgc ctgtttcgcc tcggcgtccg gactagaggt acctttaaga
     5941 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6001 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6061 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6121 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6181 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6241 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6301 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6361 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6421 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     6481 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6541 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6601 tttcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6661 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6721 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6781 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6841 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6901 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6961 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7021 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7081 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7141 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7201 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7261 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7321 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7381 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7441 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7501 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7561 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7621 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7681 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7741 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7801 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7861 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7921 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7981 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8041 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8281 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8341 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8401 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8461 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8521 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     8581 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     8641 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     8701 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     8761 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     8821 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     8881 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     8941 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     9001 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9061 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9121 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     9181 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     9241 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     9301 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     9361 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     9421 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     9481 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     9541 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     9601 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     9661 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     9721 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     9781 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     9841 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     9901 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     9961 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    10021 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    10081 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    10141 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    10201 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    10261 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    10321 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    10381 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    10441 gacccacctc ccaaccccga ggggac
//
