LOCUS       sequence-26519-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Hackett EA, Esch RK, Maleri S, Errede B
  TITLE     A family of destabilized cyan fluorescent proteins as 
            transcriptional reporters in S. cerevisiae.
  JOURNAL   Yeast. 2006 Apr 15. 23(5):333-49.
  PUBMED    16598699
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Apr 15. 23(5):333-49."
COMMENT     Sequence Label: pNC953
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(114..341)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(750..773)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 agaattggga caactccngt gaaaagttct tctcctttac tcgtattggg cgccngggtg
       61 gtttttcttt tcaccagtga gacgggcaac agccaagctc cggatccgtg ttcaccacct
      121 cttagcctta gcacaagatg taaggtggac tccttctgaa tgttgtaatc agacagcgtt
      181 ctaccgtctt ctagctgctt accggcaaag atcaatcttt gttgatctgg agggatacct
      241 tccttgtctt gaattttcga cttaacgttg tcgatggtat cggaagattc aacttccaat
      301 gttatggttt taccggtcaa agtcttgacg aaaatctgca taatctatta gttaaagtaa
      361 agtgggaggg agagttcgag agttcttttc gtattgtagt agatagaaga tatacttatc
      421 cttatccgta cagttcttgc tctttctgaa gtagcacttt cggaagaaga aagataagca
      481 ggcgaattga tccggtaatt tagtgtgtgt atttgtgttt gcgtgtctat agaagtatag
      541 taatttatgc tacaaaggac ctaatgtata aggaaagaat atttagagaa aagaagaaaa
      601 caagagtttt atatacatac agagcacatg catgccatat gatcatgtgt cgtcgcacac
      661 atatatatat gcctgtatgt gtcagcacta aagttgcctg gccatccacg ctatatacac
      721 gcctggcaga tccggggttt tttctccttg acgttaaagt atagaggtat attaacaatt
      781 ttttgttgat acttttatta catttgaata agaagtaata caaaccgaaa atgntgaaag
      841 tattagttaa agtggntatg cagttt
//
