LOCUS       sequence-265191-        3203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE1-ahpCp-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: TJF66
FEATURES             Location/Qualifiers
     source          1..3203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             322..333
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             340..1050
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(485..503)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     753..772
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1086..1172
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1337..1925)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1417..1436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2013..2107)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2137..2928,2929..2997))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2760..2779
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2998..3102)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3170..3188
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgaggctta gatcaggtga ttgccctttg tttatgaggg tgttgtaatc catgtcgttg
       61 ttgcatttgt aagggcaaca cctcagcctg caggcaggca ctgaagatac caaagggtag
      121 ttcagattac acggtcacct ggaaaggggg ccattttact ttttatcgcc gctggcggtg
      181 caaagttcac aaagttgtct tacgaaggtt gtaaggtaaa acttatcgat ttgataatgg
      241 aaacgcatta gccgaatcgg caaaaattgg ttaccttaca tctcatcgaa aacacggagg
      301 aagtatagat gcgaattcat taaagaggag aaaggtacca tggtgagcaa gggcgaagaa
      361 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
      421 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
      481 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
      541 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
      601 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
      661 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
      721 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
      781 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
      841 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
      901 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
      961 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     1021 accggcggca tggacgagct gtacaagtaa cccgggggat cccatggtac gcgtgctaga
     1081 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1141 tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagacctag ggcgttcggc
     1201 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1261 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1321 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1381 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1441 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1501 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1561 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1621 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1681 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1741 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     1801 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1861 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1921 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1981 gttaagggat tttggtcatg actagtgctt ggattctcac caataaaaaa cgcccggcgg
     2041 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     2101 aggagtccaa gcgagctcgt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2161 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2221 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2281 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2341 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2401 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2461 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2521 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2581 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2641 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2701 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2761 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     2821 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     2881 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     2941 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3001 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3061 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3121 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     3181 cacgaggccc tttcgtcttc acc
//
