LOCUS       sequence-265195-        4225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE1-oxySp-mCherry-proD-oxyR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: TJF229
FEATURES             Location/Qualifiers
     source          1..4225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             112..123
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             130..840
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(275..293)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     543..562
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      876..962
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2105..2191
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2355..2943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2435..2454)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3031..3125)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3155..3946,3947..4015))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3778..3797
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4016..4120)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4188..4206
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gttcattatc catcctccat cgccacgata gttcatggcg ataggtagaa tagcaatgaa
       61 cgattatccc tatcaagcat tctgactgat aattgctcac acgaattcat taaagaggag
      121 aaaggtacca tggtgagcaa gggcgaagaa gataacatgg ccatcatcaa ggagttcatg
      181 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      241 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      301 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      361 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
      421 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      481 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      541 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
      601 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
      661 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
      721 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
      781 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
      841 cccgggggat cccatggtac gcgtgctaga ggcatcaaat aaaacgaaag gctcagtcga
      901 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
      961 atccgccgcc ctagacctag cacagctaac accacgtcgt ccctatctgc tgccctaggt
     1021 ctatgagtgg ttgctggata actttacggg catgcataag gctcgtataa tatattcagg
     1081 gagaccacaa cggtttccct ctacaaataa ttttgtttaa ctttgaattc ttcacacagg
     1141 aaaccggtac catgaatatt cgtgatcttg agtacctggt ggcattggct gaacaccgcc
     1201 attttcggcg tgcggcagat tcctgccacg ttagccagcc gacgcttagc gggcaaattc
     1261 gtaagctgga agatgagctg ggcgtgatgt tgctggagcg gaccagccgt aaagtgttgt
     1321 tcacccaggc gggaatgctg ctggtggatc aggcgcgtac cgtgctgcgt gaggtgaaag
     1381 tccttaaaga gatggcaagc cagcagggcg agacgatgtc cggaccgctg cacattggtt
     1441 tgattcccac agttggaccg tacctgctac cgcatattat ccctatgctg caccagacct
     1501 ttccaaagct ggaaatgtat ctgcatgaag cacagaccca ccagttactg gcgcaactgg
     1561 acagcggcaa actcgattgc gtgatcctcg cgctggtgaa agagagcgaa gcattcattg
     1621 aagtgccgtt gtttgatgag ccaatgttgc tggctatcta tgaagatcac ccgtgggcga
     1681 accgcgaatg cgtaccgatg gccgatctgg caggggaaaa actgctgatg ctggaagatg
     1741 gtcactgttt gcgcgatcag gcaatgggtt tctgttttga agccggggcg gatgaagata
     1801 cacacttccg cgcgaccagc ctggaaactc tgcgcaacat ggtggcggca ggtagcggga
     1861 tcactttact gccagcgctg gctgtgccgc cggagcgcaa acgcgatggg gttgtttatc
     1921 tgccgtgcat taagccggaa ccacgccgca ctattggcct ggtttatcgt cctggctcac
     1981 cgctgcgcag ccgctatgag cagctggcag aggccatccg cgcaagaatg gatggccatt
     2041 tcgataaagt tttaaaacag gcggtttaac ccgggggatc ccatggtacg cgtgctagag
     2101 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2161 gtcggtgaac gctctcctga gtaggacaaa tccgccgccc tagactaggg cgttcggctg
     2221 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2281 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2341 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2401 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2461 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2521 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2581 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2641 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2701 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2761 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     2821 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2881 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2941 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3001 taagggattt tggtcatgac tagtgcttgg attctcacca ataaaaaacg cccggcggca
     3061 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
     3121 gagtccaagc gagctcgtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3181 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3241 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3301 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3361 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3421 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3481 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3541 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3601 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3661 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3721 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3781 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3841 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3901 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3961 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4021 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4081 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4141 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     4201 cgaggccctt tcgtcttcac ctcga
//
