LOCUS       sequence-265196-        4234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE1-oxySp-sfGFP-proD-oxyR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: pIM09
FEATURES             Location/Qualifiers
     source          1..4234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             112..123
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             130..843
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      885..971
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2114..2200
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2364..2952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2444..2463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3040..3134)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3164..3955,3956..4024))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3787..3806
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4025..4129)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4197..4215
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gttcattatc catcctccat cgccacgata gttcatggcg ataggtagaa tagcaatgaa
       61 cgattatccc tatcaagcat tctgactgat aattgctcac acgaattcat taaagaggag
      121 aaaggtacca tgcgtaaagg cgaagagctg ttcactggtg tcgtccctat tctggtggaa
      181 ctggatggtg atgtcaacgg tcataagttt tccgtgcgtg gcgagggtga aggtgacgca
      241 actaatggta aactgacgct gaagttcatc tgtactactg gtaaactgcc ggtaccttgg
      301 ccgactctgg taacgacgct gacttatggt gttcagtgct ttgctcgtta tccggaccat
      361 atgaagcagc atgacttctt caagtccgcc atgccggaag gctatgtgca ggaacgcacg
      421 atttccttta aggatgacgg cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat
      481 accctggtaa accgcattga gctgaaaggc attgacttta aagaagacgg caatatcctg
      541 ggccataagc tggaatacaa ttttaacagc cacaatgttt acatcaccgc cgataaacaa
      601 aaaaatggca ttaaagcgaa ttttaaaatt cgccacaacg tggaggatgg cagcgtgcag
      661 ctggctgatc actaccagca aaacactcca atcggtgatg gtcctgttct gctgccagac
      721 aatcactatc tgagcacgca aagcgttctg tctaaagatc cgaacgagaa acgcgatcat
      781 atggttctgc tggagttcgt aaccgcagcg ggcatcacgc atggtatgga tgaactgtac
      841 aaatgatgac ccgggggatc ccatggtacg cgtgctagag gcatcaaata aaacgaaagg
      901 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
      961 gtaggacaaa tccgccgccc tagacctagc acagctaaca ccacgtcgtc cctatctgct
     1021 gccctaggtc tatgagtggt tgctggataa ctttacgggc atgcataagg ctcgtataat
     1081 atattcaggg agaccacaac ggtttccctc tacaaataat tttgtttaac tttgaattct
     1141 tcacacagga aaccggtacc atgaatattc gtgatcttga gtacctggtg gcattggctg
     1201 aacaccgcca ttttcggcgt gcggcagatt cctgccacgt tagccagccg acgcttagcg
     1261 ggcaaattcg taagctggaa gatgagctgg gcgtgatgtt gctggagcgg accagccgta
     1321 aagtgttgtt cacccaggcg ggaatgctgc tggtggatca ggcgcgtacc gtgctgcgtg
     1381 aggtgaaagt ccttaaagag atggcaagcc agcagggcga gacgatgtcc ggaccgctgc
     1441 acattggttt gattcccaca gttggaccgt acctgctacc gcatattatc cctatgctgc
     1501 accagacctt tccaaagctg gaaatgtatc tgcatgaagc acagacccac cagttactgg
     1561 cgcaactgga cagcggcaaa ctcgattgcg tgatcctcgc gctggtgaaa gagagcgaag
     1621 cattcattga agtgccgttg tttgatgagc caatgttgct ggctatctat gaagatcacc
     1681 cgtgggcgaa ccgcgaatgc gtaccgatgg ccgatctggc aggggaaaaa ctgctgatgc
     1741 tggaagatgg tcactgtttg cgcgatcagg caatgggttt ctgttttgaa gccggggcgg
     1801 atgaagatac acacttccgc gcgaccagcc tggaaactct gcgcaacatg gtggcggcag
     1861 gtagcgggat cactttactg ccagcgctgg ctgtgccgcc ggagcgcaaa cgcgatgggg
     1921 ttgtttatct gccgtgcatt aagccggaac cacgccgcac tattggcctg gtttatcgtc
     1981 ctggctcacc gctgcgcagc cgctatgagc agctggcaga ggccatccgc gcaagaatgg
     2041 atggccattt cgataaagtt ttaaaacagg cggtttaacc cgggggatcc catggtacgc
     2101 gtgctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     2161 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct agactagggc
     2221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2761 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     2821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3001 aactcacgtt aagggatttt ggtcatgact agtgcttgga ttctcaccaa taaaaaacgc
     3061 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     3121 tatcaacagg agtccaagcg agctcgtaaa cttggtctga cagttaccaa tgcttaatca
     3181 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3241 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3301 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3361 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3421 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3481 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3541 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3601 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3661 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3721 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3781 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3841 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3901 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3961 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4021 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4081 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4141 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     4201 ggcgtatcac gaggcccttt cgtcttcacc tcga
//
