LOCUS       sequence-265202-        2995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE1-oxySp-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: TJF117
FEATURES             Location/Qualifiers
     source          1..2995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             112..123
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             130..840
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(275..293)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     543..562
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      875..961
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1125..1713)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1205..1224)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1801..1895)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1925..2716,2717..2785))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2548..2567
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2786..2890)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2958..2976
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gttcattatc catcctccat cgccacgata gttcatggcg ataggtagaa tagcaatgaa
       61 cgattatccc tatcaagcat tctgactgat aattgctcac acgaattcat taaagaggag
      121 aaaggtacca tggtgagcaa gggcgaagaa gataacatgg ccatcatcaa ggagttcatg
      181 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      241 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      301 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      361 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
      421 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      481 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      541 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
      601 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
      661 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
      721 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
      781 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
      841 cccgggggat cccatggtac gcgtctagag gcatcaaata aaacgaaagg ctcagtcgaa
      901 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
      961 tccgccgccc tagactaggg cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1021 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1081 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1141 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1201 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1261 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1321 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1381 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1441 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1501 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1561 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1621 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1681 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1741 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgac tagtgcttgg
     1801 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1861 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgtaa acttggtctg
     1921 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1981 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2041 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2101 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2161 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2221 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2281 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2341 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2401 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2461 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2521 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2581 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2641 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2701 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2761 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2821 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2881 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     2941 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac ctcga
//
