LOCUS       sequence-265204-        3216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE3-pLsoxS-soxR-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: JRF145
FEATURES             Location/Qualifiers
     source          1..3216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             80..91
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             572..1279
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(714..732)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     982..1001
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1309..1395
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1560..2148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1640..1659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2236..2330)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2351..3010)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2925..2944
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3011..3113)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3179..3197
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gccataaatc gctttacctc aagttaactt gaggaattat actccccaac atcagcagga
       61 cgcactgacc gaattcatta aagaggagaa aggtaccatg gaaaagaaat taccccgcat
      121 taaagcgctg ctaacccccg gcgaagtggc gaaacgcagc ggtgtggcgg tatcggcgct
      181 gcatttctat gaaagtaaag ggttgattac cagtatccgt aacagcggca atcagcggcg
      241 atataaacgt gatgtgttgc gatatgttgc aattatcaaa attgctcagc gtattggcat
      301 tccgctggcg accattggtg aagcgtttgg cgtgttgccc gaagggcata cgttaagtgc
      361 gaaagagtgg aaacagcttt cgtcccaatg gcgagaagag ttggatcggc gcattcatac
      421 cttagtggcg ctgcgtgacg aactggacgg atgtattggt tgtggctgcc tttcgcgcag
      481 tgattgcccg ttgcgtaacc cgggcgaccg cttaggagaa gaaggtaccg gcgcacgctt
      541 gctggaagat gaacaaaacg gtggttctgg tgtgagcaag ggcgaagaag ataacatggc
      601 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      661 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      721 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
      781 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
      841 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      901 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
      961 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1021 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     1081 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     1141 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     1201 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     1261 ggacgagctg tacaagtaag gatcccatgg tacgcgtgct agaggcatca aataaaacga
     1321 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1381 ctgagtagga caaatccgcc gccctagacc tagggcgttc ggctgcggcg agcggtatca
     1441 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1501 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1561 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1621 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1681 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1741 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1801 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1861 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1921 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1981 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2041 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2101 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2161 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2221 atgactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     2281 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     2341 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     2401 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     2461 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     2521 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     2581 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     2641 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     2701 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     2761 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
     2821 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     2881 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     2941 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     3001 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     3061 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
     3121 attttcgcca gatatcgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     3181 taggcgtatc acgaggccct ttcgtcttca cctcga
//
