LOCUS       sequence-265207-        3078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZA4-pLsoxS-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: pIM10
FEATURES             Location/Qualifiers
     source          1..3078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             99..110
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             117..827
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(262..280)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     530..549
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      863..949
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(986..1003)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1120..1665)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1200..1219)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1785..1879)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1929..2720)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     3060..3078
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cctttcgtct tcacctcgag ccataaatcg ctttacctca agttaacttg aggaattata
       61 ctccccaaca tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatgg
      121 tgagcaaggg cgaagaagat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
      181 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      241 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
      301 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
      361 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      421 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
      481 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
      541 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
      601 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
      661 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
      721 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
      781 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaaccc gggggatccc
      841 atggtacgcg tgctagaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      901 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta
      961 gacctagggg gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     1021 tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga
     1081 tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc
     1141 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata
     1201 aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg
     1261 gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca
     1321 gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg
     1381 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag
     1441 caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc
     1501 ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct
     1561 cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt
     1621 tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt
     1681 aatcagataa aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
     1741 gtcagcccca tacgatataa gttgttacta gtactagtgc ttggattctc accaataaaa
     1801 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     1861 ggatctatca acaggagtcc aagcgagctc gagctcgata tccgtcggct tgaacgaatt
     1921 gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
     1981 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
     2041 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
     2101 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
     2161 actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
     2221 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
     2281 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
     2341 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
     2401 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
     2461 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
     2521 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
     2581 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
     2641 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
     2701 gcgatcaccg cttccctcat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta
     2761 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     2821 gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc
     2881 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     2941 cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc
     3001 atccggatat cgacgtcgac gtctaagaaa ccattattat catgacatta acctataaaa
     3061 ataggcgtat cacgaggc
//
