LOCUS       sequence-26521-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Hackett EA, Esch RK, Maleri S, Errede B
  TITLE     A family of destabilized cyan fluorescent proteins as 
            transcriptional reporters in S. cerevisiae.
  JOURNAL   Yeast. 2006 Apr 15. 23(5):333-49.
  PUBMED    16598699
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Apr 15. 23(5):333-49."
COMMENT     Sequence Label: pNC987
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(328..344)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(328..344)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(341..363)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    352..368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(376..382,383..400,401..406))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    421..442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(559..576)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtcattatt aatttagtgt gtgtatttgt gtttgcgtgt ctatagaagt atagtaattt
       61 atgctacaaa ggacctaatg tataaggaaa gaatatttag agaaaagaag aaaacaagag
      121 ttttatatac atacagagca catgcatgcc atatgatcat gtgtcgtcgc acacatatat
      181 atatgcctgt atgtgtcagc actaaagttg cctggccatc cacgctatat acacgcctgg
      241 cagatctgct cgatttgttt caaattacag acgtttcatg tttgtttcat cactcgaggg
      301 gggtaccgag ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
      361 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      421 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      541 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      601 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      661 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      781 tcanangtgg cgaaacccga caggactata aanataccnn gcgtttcccc ctggaagctc
      841 cctcgtgcgc tctcctgntc cnacctgccn nttancggan anctgtccgc ctttctccct
      901 ngg
//
