LOCUS       sequence-265211-       13363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cardiac-specific overexpression of Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13363)
  AUTHORS   Johansen AK, Molenaar B, Versteeg D, Leitoguinho AR, Demkes C, 
            Spanjaard B, de Ruiter H, Akbari Moqadam F, Kooijman L, Zentilin L, 
            Giacca M, van Rooij E
  TITLE     Postnatal Cardiac Gene Editing Using CRISPR/Cas9 With AAV9-Mediated 
            Delivery of Short Guide RNAs Results in Mosaic Gene Disruption.
  JOURNAL   Circ Res. 2017 Oct 27;121(10):1168-1181. doi: 
            10.1161/CIRCRESAHA.116.310370. Epub 2017 Aug 29.
  PUBMED    28851809
REFERENCE   2  (bases 1 to 13363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1161/CIRCRESAHA.116.310370"; journalName: "Circ Res"; date: 
            "2017-10-27- 27"; volume: "121"; issue: "10"; pages: "1168-1181"
COMMENT     Sequence Label: Myh6-Cas9
FEATURES             Location/Qualifiers
     source          1..13363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..68
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     60..77
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     61..77
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     84..103
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        84..102
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     137..153
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      5591..5600
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5600..5665
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5672..5692
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5717..9817
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5717..9817
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9818..9865
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    9911..10533
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(10110..10129)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(10548..10564)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(10636..10654)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10675..10691)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10675..10691)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10688..10710)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10699..10715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10723..10729,10730..10747,10748..10753))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10768..10789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10906..10923)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11077..11665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11157..11176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11836..12627,12628..12696))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12459..12478
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12697..12801)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(12827..13282)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12964..12985)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13176..13195
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
       61 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggtacctcca
      121 ccgcggtggc ggccgctcta gaactagtgg atcctgcaag gtcacacaag ggtctccacc
      181 caccaggtgc cctagtctca atttcagttt ccatgccttg ttctcacaat gctggcctcc
      241 ccagagctaa tttggacttt gtttttattt caaaagggcc tgaatgagga gtagatcttg
      301 tgctacccag ctctaagggt gcccgtgaag ccctcagacc tggagccttt gcaacagccc
      361 tttaggtgga agcagaataa agcaattttc cttaaagcca aaatcctgcc tctagactct
      421 tcttctctga cctcggtccc tgggctctag ggtggggagg tggggcttgg aagaagaagg
      481 tggggaagtg gcaaaagccg atccctaggg ccctgtgaag ttcggagcct tccctgtaca
      541 gcactggctc atagatcctc ctccagccaa acatagcaag aagtgatacc tcctttgtga
      601 cttccccagg cccagtacct gtcaggttga aacaggattt agagaagcct ctgaactcac
      661 ctgaactctg aagctcatcc accaagcaag cacctaggtg ccactgctag ttagtatcct
      721 acgctgataa tatgcagagc tgggccacag aagtcctggg gtgtaggaac tgaccagtga
      781 cttttcagtc ggcaaaggta tgaccccctc agcagatgta gtaatgtccc cttagatccc
      841 atcccaggca ggtctctaag aggacatggg atgagagatg tagtcatgtg gcattccaaa
      901 cacagctatc cacagtgtcc cttgcccctt ccacttagcc aggaggacag taaccttagc
      961 ctatctttct tcctccccat cctcccagga cacaccccct ggtctgcagt attcatttct
     1021 tccttcacgt cccctctgtg acttccattt gcaaggcttt tgacctctgc agctgctgga
     1081 agatagagtt tggccctagg tgtggcaagc catctcaaga gaaagcagac aacaggggga
     1141 ccagattttg gaaggatcag gaactaaatc actggcgggc ctgggggtag aaaaaagagt
     1201 gagtgagtcc gctccagcta agccaagcta gtccccgaga tactctgcca cagctgggct
     1261 gctcggggta gctttaggaa tgtgggtctg aaagacaatg ggattggaag acatctcttt
     1321 gagtctcccc tcaaccccac ctacagacac actcgtgtgt ggccagactc ctgttcaaca
     1381 gccctctgtg ttctgaccac tgagctaggc aaccagagca tgggccctgt gctgaggatg
     1441 aagagttggt taccaatagc aaaaacagca ggggagggag aacagagaac gaaataagga
     1501 aggaagaagg aaaggccagt caatcagatg cagtcagaag agatgggaag ccaacacaca
     1561 gcttgagcag aggaaacaga aaagggagag attctgggca taaggaggcc acagaaagaa
     1621 gagcccaggc cccccaagtc tcctctttat accctcatcc cgtctcccaa ttaagcccac
     1681 tcttcttcct agatcagacc tgagctgcag cgaagagacc cgtagggagg atcacactgg
     1741 atgaaggaga tgtgtggaga agtccaggga acctaagagc cagagcctaa aagagcaaga
     1801 gataaaggtg cttcaaaggt ggccaggctg tgcacacaga gggtcgagga ctggtggtag
     1861 agcctcaaga taaggatgat gctcagaatg ggcggggggg gggattctgg gggggggaga
     1921 gagaaggtga gaaggagcct ggaacagaga atctggaagc gctggaaacg ataccataaa
     1981 gggaagaacc caggctacct ttagatgtaa atcatgaaag acagggagaa gggaagctgg
     2041 agagagtaga aggaccccgg ggcaagacat ggaagcaagg acaagccagg ttgagcgctc
     2101 cgtgaaatca gcctgctgaa ggcagagccc tggtatgagc accagaacag cagaggctag
     2161 ggttaatgtc gagacaggga acagaaggta gacacaggaa cagacagaga cgggggagcc
     2221 aggtaacaaa ggaatggtcc ttctcacctg tggccagagc gtccatctgt gtccacatac
     2281 tctagaatgt tcatcagact gcagggctgg cttgggaggc agctggaaag agtatgtgag
     2341 agccagggga gacaaggggg cctaggaaag gaagaagagg gcaaaccagg ccacacaaga
     2401 gggcagagcc cagaactgag ttaactcctt ccttgttgca tcttccatag gaggcagtgg
     2461 gaactctgtg accaccatcc cccatgagcc cccactaccc ataccaagtt tggcctgagt
     2521 ggcattctag gttccctgag gacagagcct ggcctttgtc tcttggacct gacccaagct
     2581 gacccaatgt tctcagtacc ttatcatgcc ctcaagagct tgagaaccag gcagtgacat
     2641 attaggccat gggctaaccc tggagcttgc acacaggagc ctcaagtgac ctccagggac
     2701 acagctgcag acaggtggcc tttatcccca aagagcaacc atttggcata ggtggctgca
     2761 aatgggaatg caaggttgaa tcaggtccct tcaagaatac tgcatgcaag acctaagacc
     2821 cctggagaga ggggtatgct cctgccccca cccaccataa ggggagtgaa ctatcctagg
     2881 gggctggcga ccttggggag acaccacatt actgagagtg ctgagcccag aaaaactgac
     2941 cgccctgtgt cctgcccacc tccacactct agagctatat tgagaggtga cagtagatag
     3001 ggtgggagct ggtagcaggg agagtgttcc tgggtgtgag ggtgtagggg aaagccagag
     3061 caggggagtc tggctttgtc tcctgaacac aatgtctact tagttataac aggcatgacc
     3121 tgctaaagac ccaacatcta cgacctctga aaagacagca gccctggagg acaggggttg
     3181 tctctgagcc ttgggtgctt gatggtgcca caaaggaggg catgagtgtg agtataaggc
     3241 cccaggagcg ttagagaagg gcacttggga aggggtcagt ctgcagagcc cctatccatg
     3301 gaatctggag cctggggcca actggtgtaa atctctgggc ctgccaggca ttcaaagcag
     3361 cacctgcatc ctctggcagc ctggggaggc ggaagggagc aaccccccac ttataccctt
     3421 tctccctcag ccccaggatt aacacctctg gccttccccc ttcccacctc ccatcaggag
     3481 tggagggttg cagagggagg gtaaaaacct acatgtccaa acatcatggt gcacgatata
     3541 tggatcagta tgtgtagagg caagaaagga aatctgcagg cttaactggg ttaatgtgta
     3601 aagtctgtgt gcatgtgtgt gtgtctgact gaaaacgggc atggctgtgc agctgttcag
     3661 ttctgtgcgt gaggttacca gactgcaggt ttgtgtgtaa attgcccaag gcaaagtggg
     3721 tgaatccctt ccatggttta aagagattgg atgatggcct gcatctcaag gaccatggaa
     3781 aatagaatgg acactctata tgtgtctcta agctaaggta gcaaggtctt tggaggacac
     3841 ctgtctagag atgtgggcaa cagagactac agacagtatc tgtacagagt aaggagagag
     3901 aggagggggt gtagaattct cttactatca aagggaaact gagtcgtgca cctgcaaagt
     3961 ggatgctctc cctagacatc atgactttgt ctctggggag ccagcactgt ggaacttcag
     4021 gtctgagaga gtaggaggct cccctcagcc tgaagctatg cagatagcca gggttgaaag
     4081 ggggaaggga gagcctggga tgggagcttg tgtgttggag gcaggggaca gatattaagc
     4141 ctggaagaga aggtgaccct tacccagttg ttcaactcac ccttcagatt aaaaataact
     4201 gaggtaaggg cctgggtagg ggaggtggtg tgagacgctc ctgtctctcc tctatctgcc
     4261 catcggccct ttggggagga ggaatgtgcc caaggactaa aaaaaggcca tggagccaga
     4321 ggggcgaggg caacagacct ttcatgggca aaccttgggg ccctgctgtc ctcctgtcac
     4381 ctccagagcc aagggatcaa aggaggagga gccaggacag gagggaagtg ggagggaggg
     4441 tcccagcaga ggactccaaa tttaggcagc aggcatatgg gatgggatat aaaggggctg
     4501 gagcactgag agctgtcaga gatttctcca acccaggtaa gagggagttt cgggtggggg
     4561 ctcttcaccc acaccagacc tctccccacc tagaaggaaa ctgcctttcc tggaagtggg
     4621 gttcaggccg gtcagagatc tgacagggtg gccttccacc agcctgggaa gttctcagtg
     4681 gcaggaggtt tccacaagaa acactggatg ccccttccct tacgctgtct tctccatctt
     4741 cctcctgggg atgctcctcc ccgtcttggt ttatcttggc tcttcgtctt cagcaagatt
     4801 tgccctgtgc tgtccactcc atctttctct actgtctccg tgccttgcct tgccttcttg
     4861 cgtgtccttc ctttccaccc atttctcact tcaccttttc tccccttctc atttgtattc
     4921 atccttcctt ccttccttcc ttccttcctt ccttccttcc ttccttcctt tctcccttcc
     4981 ttccttcctt ccttccttcc ttccttcctt ccttcctgtg tcagagtgct gagaatcaca
     5041 cctggggttc ccacccttat gtaaacaatc ttccagtgag ccacagcttc agtgctgctg
     5101 ggtgctctct taccttcctc accccctggc ttgtcctgtt ccatcctggt caggatctct
     5161 agattggtct cccagcctct gctactcctc ttcctgcctg ttcctctctc tgtccagctg
     5221 cgccactgtg gtgcctcgtt ccagctgtgg tccacattct tcaggattct ctgaaaagtt
     5281 aaccaggtga gaatgtttcc cctgtagaca gcagatcacg attctcccgg aagtcaggct
     5341 tccagccctc tctttctctg cccagctgcc cggcactctt agcaaacctc aggcaccctt
     5401 accccacata gacctctgac agagaagcag gcactttaca tggagtcctg gtgggagagc
     5461 cataggctac ggtgtaaaag aggcagggaa gtggtggtgt aggaaagtca ggacttcaca
     5521 tagaagccta gcccacacca gaaatgacag acagatccct cctatctccc ccataagagt
     5581 ttgagtcgac gccaccatgg actataagga ccacgacgga gactacaagg atcatgatat
     5641 tgattacaaa gacgatgacg ataagatggc cccaaagaag aagcggaagg tcggtatcca
     5701 cggagtccca gcagccgaca agaagtacag catcggcctg gacatcggca ccaactctgt
     5761 gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg
     5821 caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg
     5881 cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa
     5941 gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag
     6001 cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca
     6061 ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta
     6121 ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct
     6181 ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc
     6241 cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt
     6301 cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact
     6361 gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg
     6421 cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt
     6481 cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga
     6541 caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct
     6601 gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc
     6661 cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa
     6721 agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa
     6781 gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat
     6841 caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga
     6901 ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct
     6961 gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa
     7021 ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc
     7081 caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg
     7141 gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac
     7201 caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga
     7261 gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa
     7321 gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa
     7381 ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga
     7441 ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga
     7501 tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct
     7561 ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct
     7621 gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata
     7681 caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg
     7741 caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct
     7801 gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca
     7861 gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg
     7921 catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc
     7981 cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa
     8041 cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct
     8101 gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct
     8161 gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta
     8221 cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt
     8281 gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt
     8341 gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa
     8401 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
     8461 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
     8521 ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
     8581 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
     8641 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
     8701 cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta
     8761 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
     8821 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
     8881 gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
     8941 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
     9001 aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag
     9061 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag
     9121 ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
     9181 actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa
     9241 gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat
     9301 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
     9361 tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
     9421 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
     9481 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
     9541 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
     9601 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
     9661 caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta
     9721 caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
     9781 cgagacacgg atcgacctgt ctcagctggg aggcgacaaa aggccggcgg ccacgaaaaa
     9841 ggccggccag gcaaaaaaga aaaagtaaga attcctagag ctcgctgatc agcctacgcg
     9901 taagcttgat gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
     9961 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
    10021 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
    10081 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
    10141 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
    10201 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg
    10261 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat
    10321 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc
    10381 tgtccttctg attttaaaat aactatacca gcaggaggac gtccagacac agcataggct
    10441 acctggccat gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg
    10501 cgttgggtcc actcagtaga tgcctgttga attcctgcag cccgggggat ccactagttc
    10561 tagagcggcc gtttaaacta gtatacacgt ggcgcccggg cggccgccac cgcggtggag
    10621 ctccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
    10681 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    10741 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    10801 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    10861 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    10921 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    10981 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    11041 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    11101 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11161 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    11221 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    11281 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    11341 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    11401 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    11461 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    11521 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    11581 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    11641 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    11701 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    11761 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    11821 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    11881 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    11941 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    12001 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    12061 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    12121 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    12181 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    12241 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    12301 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    12361 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    12421 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    12481 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    12541 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    12601 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    12661 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    12721 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    12781 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
    12841 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
    12901 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
    12961 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
    13021 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
    13081 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
    13141 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
    13201 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
    13261 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
    13321 cgatcggtgc gggcctcttc gctattacgc cagctggcga aag
//
