LOCUS       sequence-265212-        3093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZA4-oxySp-mCherry-TEVrs-LAA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: pIM23
FEATURES             Location/Qualifiers
     source          1..3093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             112..123
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             130..837
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(275..293)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     543..562
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             844..864
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(1063..1080)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1197..1742)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1277..1296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1862..1956)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2006..2797)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cttcattatc catcctccat cgccacgata gttcatggcg ataggtagaa tagcaatgaa
       61 cgattatccc tatcaagcat tctgactgat aattgctcac acgaattcat taaagaggag
      121 aaaggtacca tggtgagcaa gggcgaagaa gataacatgg ccatcatcaa ggagttcatg
      181 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      241 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      301 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      361 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
      421 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      481 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      541 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
      601 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
      661 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
      721 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
      781 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggtc
      841 gacgaaaacc tgtacttcca gggtaggcct gcagcaaacg acgaaaacta cgctttagca
      901 gcttaacccg ggggatccca tggtacgcgt gctagaggca tcaaataaaa caaggctcag
      961 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     1021 acaaatccgc cgccctagac ctagggggat atattccgct tcctcgctca ctgactcgct
     1081 acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct
     1141 ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc
     1201 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
     1261 aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct
     1321 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
     1381 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     1441 accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1501 ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag
     1561 tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc
     1621 ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc
     1681 cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc
     1741 aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt
     1801 caaatgtagc acctgaagtc agccccatac gatataagtt gttactagta ctagtgcttg
     1861 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     1921 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgag ctcgatatcc
     1981 gtcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc tcgcctttca
     2041 cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc
     2101 caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca
     2161 ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa
     2221 tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc
     2281 atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga
     2341 gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga
     2401 tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct
     2461 gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt
     2521 gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag
     2581 tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg
     2641 taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca
     2701 aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata
     2761 gttgagtcga tacttcggcg atcaccgctt ccctcatgat gtttaacttt gttttagggc
     2821 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     2881 aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa cagtcataac
     2941 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
     3001 agttgcgtga gcgcatacgc tacttgcatt acagcttacg aaccgaacag gcttatgtcc
     3061 actgggttcg tgccttcatc cggatatcga cgt
//
