LOCUS       sequence-265215-        3977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZA4-oxySp-mCherry-pLsoxS-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: pIM16
FEATURES             Location/Qualifiers
     source          1..3977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             112..123
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             130..840
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      876..962
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             1060..1071
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1078..1788
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      1824..1910
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1947..1964)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2081..2626)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2161..2180)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2746..2840)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2890..3681)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cttcattatc catcctccat cgccacgata gttcatggcg ataggtagaa tagcaatgaa
       61 cgattatccc tatcaagcat tctgactgat aattgctcac acgaattcat taaagaggag
      121 aaaggtacca tggtgagcaa gggcgaagaa gataacatgg ccatcatcaa ggagttcatg
      181 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      241 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      301 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      361 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
      421 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      481 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      541 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
      601 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
      661 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
      721 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
      781 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
      841 cccgggggat cccatggtac gcgtgctaga ggcatcaaat aaaacgaaag gctcagtcga
      901 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
      961 atccgccgcc ctagactcga gccataaatc gctttacctc aagttaactt gaggaattat
     1021 actccccaac atcagcagga cgcactgacc gaattcatta aagaggagaa aggtaccatg
     1081 gtgagcaagg gcgaagaaga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     1141 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     1201 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     1261 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     1321 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     1381 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     1441 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     1501 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     1561 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     1621 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     1681 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     1741 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaacc cgggggatcc
     1801 catggtacgc gtgctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1861 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct
     1921 agacctaggg ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
     1981 ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag
     2041 atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc
     2101 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat
     2161 aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc
     2221 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
     2281 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
     2341 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa
     2401 gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc
     2461 cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc
     2521 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt
     2581 ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat
     2641 taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg tagcacctga
     2701 agtcagcccc atacgatata agttgttact agtactagtg cttggattct caccaataaa
     2761 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     2821 tggatctatc aacaggagtc caagcgagct cgagctcgat atccgtcggc ttgaacgaat
     2881 tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc
     2941 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
     3001 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
     3061 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
     3121 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
     3181 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
     3241 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
     3301 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
     3361 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
     3421 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
     3481 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
     3541 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
     3601 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
     3661 ggcgatcacc gcttccctca tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt
     3721 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     3781 ggatgcccga ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc
     3841 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat
     3901 acgctacttg cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcctt
     3961 catccggata tcgacgt
//
