LOCUS       sequence-26523-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Hackett EA, Esch RK, Maleri S, Errede B
  TITLE     A family of destabilized cyan fluorescent proteins as 
            transcriptional reporters in S. cerevisiae.
  JOURNAL   Yeast. 2006 Apr 15. 23(5):333-49.
  PUBMED    16598699
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Apr 15. 23(5):333-49."
COMMENT     Sequence Label: pNC1053
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(536..552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(536..552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(549..571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    560..576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    629..650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     gap             710..721
                     /estimated_length=12
     primer_bind     complement(767..784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaattgggan aactccagtg aaaanttctt ctcctttact cgtattgnnn nncnnggtgg
       61 tttttctttt caccagtgag acgggcaaca gccaagctcc ggatccccgg gtaccgggta
      121 ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt
      181 ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt
      241 ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa
      301 caataataat gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca
      361 atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt
      421 catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcnntct
      481 tngcaatgtc aacagtaccc ttagtatatt ctccagannn nnnnttcgta atcatgtcat
      541 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc annnnnnnta cnagccggaa
      601 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      661 gctcactgcc cgctttccag tcnggaaacc tgtcgtgcca gctgcattan nnnnnnnnnn
      721 nacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
      781 cgctgnnnnn nnncgtncgg ctgcng
//
