LOCUS       sequence-265231-        6984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli plasmid that contains the expression optimized genes for 
            BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Robertson KE, Truong CD, Craciunescu FM, Chiu PL, Fromme P, Hansen 
            DT
  TITLE     Membrane directed expression in Escherichia coli of BBA57 and other 
            virulence factors from the Lyme disease agent Borrelia burgdorferi.
  JOURNAL   Sci Rep. 2019 Nov 26;9(1):17606. doi: 10.1038/s41598-019-53830-x.
  PUBMED    31772280
REFERENCE   2  (bases 1 to 6984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-11-26"; volume: "9(1):17606. doi"; pages: " 
            10.1038/s41598-019-53830-x"
COMMENT     Sequence Label: pUC57-BBclone1
FEATURES             Location/Qualifiers
     source          1..6984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcgtatgc tgctggcgac catcattctg attctgacca ccggtctgct ggcggcgcag
       61 agcaaaagca agagcatgac cgaggacgat ttcgactttg ataaactgct ggcgaaagag
      121 gagagcgtgc gtcgtctgtt tggcatcggt ttcggcgttg gttacccgct ggcgaacatc
      181 accattagcg tgccgtatgt tgacattgat ctgggttacg gtggctttgt gggcctgaag
      241 ccgaacaact tcctgccgta tgtggttatg ggtgttgatc tgctgttcaa agacgaaatt
      301 cacaagaaca ccatgatcag cggtggcatc ggcattggtg cggactggag caaaggcagc
      361 ccggagaaaa gcaacgaaaa gctggaggaa gaggaagaga acgaggcgca gcaagtggcg
      421 agcctgcaga accgtattgg tgtggttatc cgtctgccgc tggttattga atacagcttt
      481 ctgaaaaaca tcgtgattgg cttcaaggcg gttgcgacca tcggcaccac catgctgctg
      541 ggcagcccga tgagctttga gggtgcgcgt ttcaactttc tgggcaccgg tttcatcaag
      601 atctacatca tgatcaagat cttcaagaaa atttacatcc tgaccctggt gctgggcatg
      661 gcgcacctga gctttgcgag cgataactac atggttcgtt gcagcaagga agaggacagc
      721 accacctgca ttgcgaaact gaaggagatc aaagaaaaga aaaactatga cctgttcagc
      781 atgggtattg gtatcggcga cccgatcgcg aacattatga tcaccattcc gtatatcaac
      841 attgattttg gttacggtgg cttcattggc ctgaagagca acaactttga aaactatctg
      901 aacggtggca tcgacgttat cttcaagaag cagatcggtc aatacatgaa gatcggtggc
      961 ggtatcggta ttggcgcgga ttggagcaaa accagcctga tcccgccgaa cgaagaggaa
     1021 gagaccgatt acgagcgtat tggcgcggtg atccgtattc cgtttatcat ggaatataac
     1081 ttcgcgaaaa acctgagcat tggtttcaag atctatccgg cggttggtcc gaccatcctg
     1141 ctgaccaaac cgagcattct gtttgagggt atcaagttca acttctttgg tttcggcttt
     1201 attaaattcg cgtttaacat gaagagccac atcctgtaca aactgatcat ttttctgacc
     1261 accagcgcgg cgatctttgc ggcggatgcg ctgaaagaaa aggacatctt caaaattaac
     1321 ccgtggatgc cgaccttcgg ttttgagaac accagcgaat ttcgtctgga catggatgag
     1381 ctggtgccgg gcttcgaaaa caaaagcaag atcaccatta aactgaagcc gtttgaggcg
     1441 aacccggaac tgggcaagga cgatccgttc agcgcgtaca ttaaagtgga ggatctggcg
     1501 ctgaaggcgg aaggtaagaa aggcgaccaa ttcaaaatcg atgttggcga cattaccgcg
     1561 cagatcaaca tgtacgactt ctttatcaag attagcacca tgaccgactt cgattttaac
     1621 aaagagagcc tgttcagctt tgcgccgatg accggtttta agagcaccta ctatggcttc
     1681 ccgagcaacg atcgtgcggt tcgtggcacc atcctggcgc gtggcaccag caaaaacatc
     1741 ggcaccattc aactgggcta caaactgccg aagctggacc tgacctttgc gatcggtggc
     1801 accggcaccg gtaaccgtaa ccaggaaaac gacaaggata ccccgtacaa caaaacctat
     1861 cagggcattc tgtatggtat ccaagcgacc tggaaaccga ttaagaacct gctggatcag
     1921 aacgaggaca ccaagagcgt gatcgcggag accccgtttg aactgaactt cggtctgagc
     1981 ggcgcgtatg gtaacgaaac ctttaacaac agcagcatca cctacagcct gaaagataag
     2041 agcgtggttg gtaacgacct gctgagcccg accctgagca acagcgcgat tctggcgagc
     2101 ttcggtgcga aatataagct gggcctgacc aaaattaacg ataagaacac ctacctgatc
     2161 ctgcagatgg gcaccgattt tggtatcgac ccgttcgcga gcgactttag cattttcggt
     2221 cacatcagca aggcggcgaa cttcaagaaa gagaccccga gcgatccgaa caagaaagcg
     2281 gaaatctttg acccgaacgg taacgcgctg aatttcagca aaaacaccga gctgggcatt
     2341 gcgtttagca ccggcgcgag catcggtttc gcgtggaaca aagataccgg cgagaaggaa
     2401 agctgggcga tcaagggcag cgatagctac agcacccgtc tgtttggtga acaagacaag
     2461 aaaagcggcg tggcgctggg tattagctat ggccagaacc tgtaccgtag caaagacacc
     2521 gagaaacgtc tgaagaccat tagcgaaaac gcgttccaaa gcctgaacgt tgagatcagc
     2581 agctatgaag ataacaagaa aggtatcatc aacggtctgg gctggatcac cagcattggc
     2641 ctgtatgaca ttctgcgtca gaaaagcgtg gagaactacc cgaccaccat cagcagcacc
     2701 accgagaaca accagaccga acaaagcagc accagcacca agaccaccac cccgaacctg
     2761 accttcgagg atgcgatgaa actgggtctg gcgctgtacc tggactatgc gatcccgatt
     2821 gcgagcatca gcaccgaagc gtacgtggtt ccgtatattg gtgcgtacat cctgggcccg
     2881 agcaacaagc tgagcagcga tgcgaccaaa atctacctga agaccggtct gagcctggag
     2941 aaactgattc gttttaccac catcagcctg ggctgggaca gcaacaacat cattgaactg
     3001 gcgaacaaga acaccaacaa cgcggcgatt ggtagcgcgt ttctgcaatt caaaatcgcg
     3061 tacagcggta gcatgaacgg caaactgcgt aaggcgctga aaatcgcgat tttcaccacc
     3121 ctgctgctgg tgatcagctg caacgcgaac atggatacca acgacaaaaa caaggcgctg
     3181 aacgagtata aactgaagaa cattagcgaa gttatcaaga acagcctgca gctggagagc
     3241 gacccgaaac tgaagaaaga gccggaaagc aacatcaacc aaagcacccc gccgatcctg
     3301 gagattgaaa aaatcgagcc gggtaaacag gaaatgagcc tgaagagcga gttcggcagc
     3361 gaaagcctga tgccgctgga agagccggaa gaggcgaaca tggcgaaaag cgaagaggaa
     3421 attgcgaaaa tccaggagaa gctgctgctg attggtgcga gcgatgaaat caccgaccag
     3481 gagctgggcg aaaacatgca aaagtttctg aacccgacca ccgttgagtt caaaatcagc
     3541 accaccacca acgaaaccat tctgaccacc atcgaggaag aggaaatcaa caacaacaag
     3601 gataagatct tcgaccacaa agaggagaac gtgaccctgg gtaacaacag cctggagaac
     3661 gcgaccctga acaagaacac cattaccctg gcgcagaacc aaaaatatac cacccacctg
     3721 aaaaacgacg ataagtttat caccaaagaa tacctgaagc aagttcgtga tagcctggac
     3781 aaggcgctga acgcgattaa aaacctggag accagcaagg agttccgtga gctggaaaac
     3841 ctgccgaaag atcaggagaa cagcccgagc gcgaagaaag agagcaccga aagcaacatt
     3901 aacaacacca acaccgagaa catcgaaatc attaaaaacc gtctgctgga ggaactgaac
     3961 aagagcgagc tgatcttcga agataccaaa gacccgctgg gcaccagcac cattaagaaa
     4021 gtgatcgacg cggcgaagaa atggcaggag aaggaaaaca gcagccaaat cgactgggat
     4081 ctgggcttca aatttcaccc gaacccgaaa ttttataaca agctgaccgc gcaagagtac
     4141 aaagtgctgg cggaaaagtt caccaaagtt aagaacgagt acaagaacac caaagagcag
     4201 ctgaaagcgg aaagcaagct gaccaccaac agcatcagca aaatcattaa cgcgaccaag
     4261 gagttcgcga accaagtgat taacctgatc ctgctggttg agaagaacta ccagatggaa
     4321 aagaaattct ttagcggttt tctgctgagc ttcctggcgc tgagcattgg tttctttatc
     4381 ggcatgcact atctggcgag caaccgtagc aacatcgtgt ttgcggagga aaaagataac
     4441 accgttcgtg cgctgcaaga cagcttccgt gaggtgagca agaaaattct gccgagcagc
     4501 gttgaagtgc acgcgaccgg tgttattaag cagagctttc cgatcccgtt ctttttcttt
     4561 gatatgccgg agttcgacag cgaacgtaaa agcaactggg cgggtagcgg cgttatcatt
     4621 ggccgtgaca gccagaagaa aagcctgttt tacgtggtta ccaacagcca cgtggttgat
     4681 aaggcgaccg agctggaagt ggttagctat gacaagaaaa agcacaaagc gaagctgatc
     4741 ggtaaagatg agaaaaagga cattgcgctg atcagcttcg aaagcgacga tgcgaccatt
     4801 aaggtggcgg atctgggcga cagcgataaa ctggagatcg gtgactgggt gatggcggtt
     4861 ggcagcccgt tccaatttag cttcaccgtg accgcgggta tcgttagcgg tctgcagcgt
     4921 agcgcgaacc cgaacctgca gagccgtaac ctgttcattc aaaccgatgc ggcgatcaac
     4981 cgtggtaaca gcggcggtcc gctggtgaac atcaagggtg aggttatcgg cattaacgcg
     5041 tggattgcga gcaacagcgg cggtaacatc ggtctgggct ttgcgattcc ggtgaacaac
     5101 atcaaaagca ccgttgactt ctttctgaag ggtaaaaaga ttgaaagcgc gtggctgggc
     5161 atcagcttct acccgctgaa aacccgtgat agcgaggtgc tgaagagcct gggtgtggaa
     5221 agcaacgacg ttagcgcggc gatcattgcg agcctgtatc cgggcagccc ggcggttaag
     5281 agcggtctgc gtgcgggcga catcattatg aaagtgaacg gtgttagcat gagcgtgttt
     5341 caagatgtta ccagctatat tagcgacttc tacgcgggcg agaaggttaa cgtggaaatc
     5401 ctgcgtggca acgtgaaaaa gaacatcgag attgtgctgg cggttcgtcc gaaagataag
     5461 gagctgagca gcagcaagat gctgccgggt tttgtggttt acccgctggt tgaggacatc
     5521 aaagcgcagc tgaacctgcg taactggatt aagggtgtgg ttgtggatta catcgacaaa
     5581 aacctggcga gcaacattaa aatgaagagc ggcgacgtga tcctgagcgt taacagcaaa
     5641 agcgtgagca acctgcgtga gttctatgat gcgctggaag ttggtaaaaa cacctacaag
     5701 atcctgcgtg gcaacgacag ctttaagatt accttcatgc gtaacaaaaa catctttaag
     5761 ctgttctttg cggcgatgct gttcgtgatg gcgtgcaaag cgtacgttga ggagaagaag
     5821 gagatcgata gcctgatgga agacgtgctg gcgctggtta acgacagcag cggcggtaaa
     5881 ttcaaggatt acaaggacaa gatcaacgag ctgaaagaaa acctgaagga catcggtaac
     5941 gcggagctga aagaaaagct gctgaacctg cagaacagct tccaagacaa actggcggcg
     6001 aagctggcgg cgctgaaggc ggcgaaaaac accattgaga acatcaccga caaggatcaa
     6061 gacattagca agcgtaagat ctggagcgaa gcgaaactgg ttggtgtgac cgttccgctg
     6121 ctgggcagca acaccagcgg taacggcgat aagatgagca aaaacgcggt ggagcagatt
     6181 gacaaggtta tcaaattcct ggaggaaggc accaacatgc gtcgtctgtt cctgctgtat
     6241 atcctgtgca gctttgtttt cctgaacctg tttgcgcaag gtagcagcag ctacattgac
     6301 aaacagaagg agctggcgat cttctactat gaagtgggcc aacgttacat caacgttggc
     6361 aagatcaaaa agggcaagct gtttcaggcg aaggcgctga aaatttaccc ggatctgaaa
     6421 aagggtttcg acatcaaact ggcggtgaag gagctggatg cgcgtattaa agacgataac
     6481 ccgaaggttg tgatgctgga agacatcaag ctggaggaaa ttccgggcat cgttcacgag
     6541 aagatcgaaa tcaacgattt caccaacgcg ccgaagattg agtacatcgc gcagcgtgaa
     6601 cgtagcaaga accaagacaa gatcatcaag ttccagttcg gcaagttcgc gcgtgcgctg
     6661 attagccgta actttgacct gttcgacagc gtgatcgcgg ataaggtgaa cgttatgggc
     6721 caatttgaaa gcaaaaacga cttcatcagc accctgagca gcgcgagcag caaagcggat
     6781 gcggacgagc tggaatacct gagcgtggac gattactatg atctgaagag cctgaaaatc
     6841 agcaagagca acgacaccag cttcgcggtg aacgttaacg cgaaaaagaa cgacgttacc
     6901 aaaaactttc cgttctggaa ggagcgtcag accctgattt ttaccaccga agacgataac
     6961 aactggttcc tgagcagcat caac
//
